Expression of Cytokeratins and Vimentin in Normal and Neoplastic Tissue from the Bovine Female Reproductive Tract

Pérez‐Martínez, Claudia; García‐Fernández, Rosa A.; Escudero, A.; Ferreras, M.C.; Iglesias, María José García

doi:10.1053/jcpa.2000.0430

Cited by 24 publications

(17 citation statements)

References 13 publications

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“…human ciliary epithelium and bovine reproductive tract) [49,50]. Usually their co-expression has been associated with drug resistance, invasion and tumor metastasis [48].…”

Section: Discussionmentioning

confidence: 99%

Triple-negative vimentin-positive heterogeneous feline mammary carcinomas as a potential comparative model for breast cancer

et al. 2014

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BackgroundHuman breast cancer is a heterogeneous disease classified by molecular subtyping into luminal A, luminal B, HER2-overexpressing, basal-like, claudin-low and normal-breast like. The routinely applied and standardized immunohistochemical-based surrogates of this classification group together the last three entities as triple-negative breast cancer (TNBCs) that show the most diverse and complex heterogeneity and represent a therapeutic challenge.In the present work 156 feline mammary lesions consisting of feline mammary carcinomas (FMCs), benign neoplasms, and hyperplastic/dysplastic tissues were evaluated histologically and by immunohistochemistry for expression of basal and luminal cytokeratins (CK), vimentin, alpha-smooth muscle actin, calponin, estrogen receptor (ER) alpha (a), and progesterone receptor (PR). Thirty-seven FMCs with 27 matched non-neoplastic controls were also investigated for gene expression of ERa, ER beta, PR, and HER2.ResultsA large group of hormone receptors (HRs)-negative aggressive carcinomas - that did not overexpress HER2 - could be distinguished from the less aggressive (10.8%) and benign (8%) HRs + tumors, that showed bilineage (luminal and myoepithelial) differentiation. Immunohistochemical evaluations of cytoplasmic filaments indicated that HRs- FMCs are vimentin+, CK14+, and CK5_6+ carcinomas that may resemble the TNBCs (basal like/claudin low) described in women. The identification of luminal and myoepithelial progenitors within the mammary ductal system suggested potential cells/sites of origin of these tumors. A diffuse and never previously described CKs/vimentin luminal cell co-expression was detected in the non-neoplastic ducts, indicating a potential bilineage progenitor.ConclusionsThese results indicate and potentially explain the high incidence of triple-negative, vimentin + aggressive tumors in cats that may used to elucidate some of the challenging features of TNBCs in women.

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“…human ciliary epithelium and bovine reproductive tract) [49,50]. Usually their co-expression has been associated with drug resistance, invasion and tumor metastasis [48].…”

Section: Discussionmentioning

confidence: 99%

Triple-negative vimentin-positive heterogeneous feline mammary carcinomas as a potential comparative model for breast cancer

et al. 2014

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“…Cytokeratin 8.13 detects cytokeratins 1,5,6,7,8,10,11,and 18. In metestrus,in vivo cytokeratins 5,6,7,8,and 18 are staining reported to stain positive, whereas vimentin staining shows !10% strongly positive epithelial cells (Perez-Martinez et al 2001).…”

Section: Immunocytochemistrymentioning

confidence: 98%

“…Cytokeratin antibody was chosen according to the previous data concerning expression in oviduct epithelial cells during different stages of the estrous cycle (Perez-Martinez et al 2001). Cytokeratin 8.13 detects cytokeratins 1,5,6,7,8,10,11,and 18.…”

Section: Immunocytochemistrymentioning

confidence: 99%

A bovine oviduct epithelial cell suspension culture system suitable for studying embryo–maternal interactions: morphological and functional characterization

Rottmayer¹,

Ulbrich²,

Kölle³

et al. 2006

Reproduction

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We established a short-term (24 h) culture system for bovine oviduct epithelial cells (BOECs), obtained on day 3.5 of the estrous cycle and evaluated the cells with respect to morphological criteria, marker gene expression, and hormone responsiveness. BOEC sheets were isolated mechanically from the ampulla with similar yields from oviducts ipsi-and contralateral to the ovulation site (57.9G4.6 and 56.4G8.0!10 6 cells). BOECs showed O95% purity and cells cultured for 24 h maintained morphological characteristics present in vivo, as determined by light microscopy, scanning electron microscopy, and transmission electron microscopy. Both secretory cells with numerous secretory granules and ciliated cells with long, well-developed, and vigorously beating kinocilia were visible. Quantitative real-time PCR failed to detect significant differences in transcript levels between ipsiand contralateral BOECs for the majority of marker genes (estrogen receptors a and b (ESR1 and ESR2), 3-hydroxy-3-methylglutaryl-coenzyme A reductase (HMGCR), oviductal glycoprotein 1 (OVGP1), progesterone receptor (PGR), and tumor rejection antigen 1 (TRA1)) throughout the 24 h culture period. However, the combined data of all time points for glutathione peroxidase 4 (GPX4), a gene previously shown to be expressed at higher levels in the ipsilateral oviduct in vivo, also indicated significantly different mRNA levels in vitro. The expression of marker genes remained stable after 6 h cell culture, indicating only a short adaptation period. Western blot analysis confirmed ESR1 and PGR protein expression throughout the culture period. In agreement with cyclic differences in vivo, estradiol-17b stimulation increased PGR transcript abundance in BOECs. Our novel culture system provides functional BOECs in sufficient quantities for holistic transcriptome and proteome studies, e.g. for deciphering early embryo-maternal communication.

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“…Interestingly, vimentin-related intermediated filament proteins were not detected in the luteal phase CL tissues on the 2-DE analysis (Figure 3A). It was reported that higher vimentin expression is required for the maintenance of functional CL in the pregnant cows (Perez-Martinez et al, 2001). Therefore, if vimentin expression was detected in the CL tissues during the earlier pregnancy phase (around the period for maternal recognition), vimentin may be used as a marker for the CL development during pregnancy in Korean native cows.…”

Section: Discussionmentioning

confidence: 99%

Protein Profile in Corpus Luteum during Pregnancy in Korean Native Cows

Chung

Kim

Han

et al. 2012

Asian Australas. J. Anim. Sci

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Steroidogenesis requires coordination of the anabolic and catabolic pathways of lipid metabolism, but the profile of proteins associated with progesterone synthesis in cyclic and pregnant corpus luteum (CL) is not well-known in cattle. In Experiment 1, plasma progesterone level was monitored in cyclic cows (n = 5) and pregnant cows (n = 6; until d-90). A significant decline in the plasma progesterone level occurred at d-19 of cyclic cows. Progesterone level in abbatoir-derived luteal tissues was also determined at d 1 to 5, 6 to 13 and 14 to 20 of cyclic cows, and d-60 and -90 of pregnant cows (n = 5 each). Progesterone level in d-60 CL was not different from those in d 6 to 13 CL and d-90 CL, although the difference between d 6 to 13 and d-90 was significant. In Experiment 2, protein expression pattern in CL at d-90 (n = 4) was compared with that in CL of cyclic cows at d 6 to 13 (n = 5). Significant changes in the level of protein expression were detected in 32 protein spots by two-dimensional polyacrylamide gel electrophoresis (2-DE), and 23 of them were identified by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF-MS). Six proteins were found only in pregnant CL, while the other 17 proteins were found only in cyclic CL. Among the above 6 proteins, vimentin which is involved in the regulation of post-implantation development was included. Thus, the protein expression pattern in CL was disorientated from cyclic luteal phase to mid pregnancy, and alterations in specific CL protein expression may contribute to the maintenance of pregnancy in Korean native cows.

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Expression of Cytokeratins and Vimentin in Normal and Neoplastic Tissue from the Bovine Female Reproductive Tract

Cited by 24 publications

References 13 publications

Triple-negative vimentin-positive heterogeneous feline mammary carcinomas as a potential comparative model for breast cancer

Triple-negative vimentin-positive heterogeneous feline mammary carcinomas as a potential comparative model for breast cancer

A bovine oviduct epithelial cell suspension culture system suitable for studying embryo–maternal interactions: morphological and functional characterization

Protein Profile in Corpus Luteum during Pregnancy in Korean Native Cows

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