Expression of cytokeratins in Warthin's tumour (adenolymphoma) of parotid glands: Specific detection of individual cytokeratin types by monoclonal antibodies

Li, C.; Okamoto, Yukiharu; Ohmura, H.; Ogawa, Kazuhiro; Shrestha, Pragya; Mori, Masahiko

doi:10.1016/0964-1955(96)00023-1

Cited by 16 publications

(24 citation statements)

References 19 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Expression of CK7 in undifferentiated epithelium, in addition to secretory epithelium, in early development may perhaps explain our observation of CK7 luminal staining of some of the ductal structures in microcystic adnexal carcinoma. 31 To date, conflicting reports exist regarding expression of CK20, a marker used to denote presence of Merkel cells, in microcystic adnexal carcinoma vs desmoplastic trichoepithelioma. 21,23 While, Abesamis-Cubillan et al 21 observed Merkel cells in all 14 cases of desmoplastic trichoepithelioma, but in none of the eight microcystic adnexal carcinoma cases, in the study by Smith et al 23 Merkel cells were not seen in either.…”

Section: Discussionmentioning

confidence: 99%

Microcystic adnexal carcinoma: an immunohistochemical reappraisal

Hoang

Dresser

Kapur³

et al. 2008

Modern Pathology

View full text Add to dashboard Cite

Even though immunohistochemical comparisons of microcystic adnexal carcinoma vs infiltrative basal cell carcinoma and desmoplastic trichoepithelioma exist, they are mostly restricted to the use of a single stain. In addition, a comparison with squamous cell carcinoma has not been reported previously. In this study, we compare the expression of cytokeratin (CK) 15, CK7, CK20, CK903, carcinoembryonic antigen (CEA), CD10, CD15 and BerEP4 in 13 microcystic adnexal carcinoma, eight desmoplastic trichoepithelioma, 10 infiltrative basal cell carcinoma, and eight squamous cell carcinoma of which five exhibited ductal differentiation. We found that the majority of microcystic adnexal carcinoma (92%) and desmoplastic trichoepithelioma (100%) cases expressed CK15 while the infiltrative basal cell carcinoma and squamous cell carcinoma cases were all negative. Forty percent of infiltrative basal cell carcinoma expressed CK7; while only two microcystic adnexal carcinoma cases (15%) and one squamous cell carcinoma with ductal differentiation (12%) expressed CK7 in the remaining three tumor categories. None of the desmoplastic trichoepithelioma expressed CK7. All tumors were strongly positive for CK903. While the neoplastic cells were negative, luminal staining of ductal structures was noted for CK7, CD15 and CEA in some of the microcystic adnexal carcinoma, desmoplastic trichoepithelioma and squamous cell carcinoma with ductal differentiation cases. Sixty percent of infiltrative basal cell carcinoma, 31% of microcystic adnexal carcinoma, and 25% of squamous cell carcinoma express CD10. BerEP4 expression was noted in 38% of microcystic adnexal carcinoma, 57% of desmoplastic trichoepithelioma, 100% of infiltrative basal cell carcinoma, and 38% of squamous cell carcinoma. In conclusion, we found CK15 to be a useful marker in distinguishing microcystic adnexal carcinoma from infiltrative basal cell carcinoma and squamous cell carcinoma with ductal differentiation. Our experience indicates that microcystic adnexal carcinoma and desmoplastic trichoepithelioma have a similar immunohistochemical profile that is, CK15 þ and BerEP4 þ /À; thus, additional studies are needed to separate these two entities.

show abstract

Section: Discussionmentioning

confidence: 99%

Microcystic adnexal carcinoma: an immunohistochemical reappraisal

Hoang

Dresser

Kapur³

et al. 2008

Modern Pathology

View full text Add to dashboard Cite

show abstract

“…[21][22][23][24] On the other hand, CK20 has been reported to be consistently absent from all salivary gland epithelial elements. 22,24,25 The pattern of CK expression in normal salivary glands has been demonstrated to be recapitulated to some extent in their benign and malignant neoplasms. Nonetheless, differences among tumors of different histopathologic type, different tumors of the same type, or even different areas of the same tumor were evident.…”

Section: Discussionmentioning

confidence: 99%

“…Such studies have shown that different CKs are localized in the various compartments of normal salivary glands, for example, CK8 and CK18 are expressed in the luminal duct and acinar cells, while CK5 and CK14 are primarily seen in basal duct cells and myoepithelial cells. [21][22][23][24] Interestingly, CK7 has been shown to be expressed to a different degree in the various epithelial salivary gland elements; more specifically, the luminal cells of the salivary ducts are strongly positive for CK7, while the acinar, basal, and myoepithelial cells stain with less intensity or, according to some investigators, not at all. [21][22][23][24] On the other hand, CK20 has been reported to be consistently absent from all salivary gland epithelial elements.…”

Section: Discussionmentioning

confidence: 99%

“…[21][22][23][24] Interestingly, CK7 has been shown to be expressed to a different degree in the various epithelial salivary gland elements; more specifically, the luminal cells of the salivary ducts are strongly positive for CK7, while the acinar, basal, and myoepithelial cells stain with less intensity or, according to some investigators, not at all. [21][22][23][24] On the other hand, CK20 has been reported to be consistently absent from all salivary gland epithelial elements. 22,24,25 The pattern of CK expression in normal salivary glands has been demonstrated to be recapitulated to some extent in their benign and malignant neoplasms.…”

Section: Discussionmentioning

confidence: 99%

“…Although several investigators have examined the expression of CKs in neoplastic lesions of salivary glands, 19,[21][22][23][24][25][26][27][28][29][30][31] to the best of our knowledge this is the first time that the combined CK7 and CK20 profile of malignant salivary gland tumors is analyzed in a sizeable group of these tumors.…”

Section: Discussionmentioning

confidence: 99%

See 2 more Smart Citations

Immunohistochemical expression of cytokeratins 7 and 20 in malignant salivary gland tumors

et al. 2004

View full text Add to dashboard Cite

On the basis of the heterogeneity of cytokeratins 7 and 20 expression in malignant epithelial tumors, the cytokeratin 7/20 immunophenotype has served as a useful diagnostic tool for discrimination of primary and/or metastatic carcinomas of unknown origin. However, the expression pattern of these cytokeratins in malignant salivary gland tumors has not been thoroughly studied. Our study material was composed of 84 malignant tumors of primary major or minor salivary gland origin. Nine histologic types of carcinoma were represented, including mucoepidermoid (26 cases), adenoid cystic (25), polymorphous low grade (11), salivary duct (8), acinic cell (4), ex mixed tumor (3), not otherwise specified (3), clear cell (2), and basal cell (2). In all, 13 cases of primary skin or mucosal squamous cell carcinoma with secondary salivary gland involvement were also examined. Immunoreactivity for cytokeratin 7 was evident in all malignant salivary gland tumors; the staining pattern was diffuse and strong in 62 cases, and focal and strong in 22 cases. In contrast, 78 cases were negative for cytokeratin 20, whereas only six cases (two mucoepidermoid, one adenoid cystic, and three salivary duct) displayed focal weak positivity. Overall, 92.9% of malignant salivary gland tumors were characterized by a cytokeratin 7 positive / 20 negative immunoprofile, the remaining 7.1% of cases being positive for both cytokeratins. The latter phenotype was more common in salivary duct carcinomas (Pr0.05). On the other hand, most squamous cell carcinomas (69%) were negative for both cytokeratins, while the remaining cases (31%) were negative for cytokeratin 20 and focally weakly positive for cytokeratin 7. We suggest that assessment of cytokeratin 7/20 immunoprofile may facilitate the differential diagnosis of (a) primary malignant salivary gland tumors from metastatic tumors, (b) metastatic salivary gland tumors, (c) primary salivary gland tumors, especially mucoepidermoid carcinomas, from squamous cell carcinomas, and (d) salivary duct carcinomas from other malignant salivary gland tumors.

show abstract

Reconstruction of pleomorphic adenoma of the salivary glands in three-dimensional collagen gel matrix culture

Fujita

Yoshida²,

Sakakura

et al. 1999

Virchows Archiv

View full text Add to dashboard Cite

The morphogenesis of salivary gland pleomorphic adenoma was examined in vitro using three-dimensional (3-D) collagen gel culture. Pleomorphic adenoma cells were isolated from three parotid gland tumours and cultured as monolayers, after which they were subcultured in floating-collagen gel sandwiches. Cells cultured in both conditions were immunohistochemically characterized and compared using antibodies against various proteins representative of each histological component of salivary glands. Monolayers had myoepithelial characteristics, being positive for vimentin and alpha-smooth muscle actin. In collagen gels, however, the cells assembled in epithelial nests, showing an architecture similar to that of pleomorphic adenoma. The nests were composed of duct-lining epithelial cells that were positive for epithelial markers, surrounded by myoepithelial cells. Collagen gel culture induces multidirectional differentiation of adenoma cells, suggesting that pleomorphic adenomas originate from stem or reserve cells.

show abstract

Expression of cytokeratins in Warthin's tumour (adenolymphoma) of parotid glands: Specific detection of individual cytokeratin types by monoclonal antibodies

Cited by 16 publications

References 19 publications

Microcystic adnexal carcinoma: an immunohistochemical reappraisal

Microcystic adnexal carcinoma: an immunohistochemical reappraisal

Immunohistochemical expression of cytokeratins 7 and 20 in malignant salivary gland tumors

Reconstruction of pleomorphic adenoma of the salivary glands in three-dimensional collagen gel matrix culture

Contact Info

Product

Resources

About