Expression of endoplasmic reticulum stress protein in rabbit condyle cartilage following anterior disk displacement

Xu, Ting; Gu, Zhiyuan; Wu, Huiling; Yao, Hua; Wang, Guohua

doi:10.1111/jop.12715

Cited by 8 publications

(5 citation statements)

References 27 publications

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“…In previous studies, we reported that ER stress pathway played an essential role in the process of apoptosis induced by excessive hydrostatic pressure on chondrocytes in the mandibular condyle (Xu et al, 2017). We also found that ER stress pathway might be involved in the development of ADD by promoting chondrocyte apoptosis (Xu et al, 2018). The conclusions drawn from our studies are consistent with previous research showing that aberrant mechanical stress leads to cartilage degeneration by triggering ER stress-mediated apoptosis in the TMJ (Li et al, 2013;Yang et al, 2020).…”

supporting

confidence: 91%

Salubrinal alleviates cartilage degradation in a rabbit temporomandibular joint osteoarthritis model

Xu,

Shuai,

et al. 2023

Oral Diseases

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ObjectiveTo investigate and explain the beneficial effects of local intra‐articular injection of Salubrinal on temporomandibular joint osteoarthritis (TMJOA) using a rabbit model of anterior disc displacement (ADD).MethodsRabbits were divided and subjected to surgical ADD. Salubrinal was administered by intra‐articular injection in the TMJ every other day for 2 and 4 weeks after operation. Histological examination and TUNEL staining were then performed. Immunohistochemistry, quantitative real‐time PCR, and Western blot analysis were employed to evaluate the expression of endoplasmic reticulum (ER) stress‐related markers, catabolic factors, extracellular matrix proteins, inflammatory factors, and nuclear factor‐kappa B (NF‐κB) activation.ResultsIn the ADD groups, we found that Salubrinal partly reversed condylar cartilage deterioration according to the histological analysis. Salubrinal reduced chondrocytes apoptosis while increased matrix components including Collagen II and Aggrecan. Meanwhile, Salubrinal downregulated the catabolic expression of MMP13, ADAMTS5, VEGF, TNF‐α, and IL‐1β. We also observed that Salubrinal inhibited ER stress activation by reducing the expression of GRP78, CHOP, ATF4, and Caspase‐12. Additionally, Salubrinal suppressed the phosphorylation of NF‐κB.ConclusionThese results indicate that Salubrinal alleviates cartilage degradation following ADD, suggesting that intra‐articular injection of Salubrinal is a potential therapeutic approach for preventing TMJOA.

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supporting

confidence: 91%

Salubrinal alleviates cartilage degradation in a rabbit temporomandibular joint osteoarthritis model

Xu,

Shuai,

et al. 2023

Oral Diseases

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“…It can cause structural damage and abnormal articular forces to induce OA-like lesions directly by using surgical and mechanical devices. The common surgical methods include discectomy ( Hinton, 1992 ; Lan et al, 2017 ; Liu X. et al, 2020 ; Saito et al, 2021 ), partial discectomy ( Man et al, 2009 ; Xu et al, 2009 ; Lei et al, 2022 ), disc perforation ( Embree et al, 2015 ; Luo et al, 2020 ; Ruscitto et al, 2020 ), anterior disc displacement ( Togni et al, 2018 ; Xu et al, 2018 ; Nguyen et al, 2020 ), injury of the condylar surface ( Ishimaru and Goss, 1992 ; Wang F. et al, 2017 ), and postero-superior displacement of the mandible ( Imai et al, 2001 ; Liu et al, 2006 ) ( Table 3 ). These six methods well mimic advanced symptoms of joint injury in clinical patients, but they are not starting factors in general TMJOA.…”

Section: Classification Of Animal Models In Temporomandibular Joint O...mentioning

confidence: 99%

“…Mice, rats, rabbits, pigs, and sheep are widely used in vivo preclinical studies as surgical induction models. Rabbits are the most used models ( Imai et al, 2001 ; Man et al, 2009 ; Embree et al, 2015 ; Xu et al, 2018 ; Saito et al, 2021 ), because they are quite large and strong enough to fight off infection. Therefore, they tolerate surgeries; their joint tissues are large enough to undergo surgery and wear mechanical devices.…”

Section: Classification Of Animal Models In Temporomandibular Joint O...mentioning

confidence: 99%

Animal Models of Temporomandibular Joint Osteoarthritis: Classification and Selection

Zhao

Zhou

et al. 2022

Front. Physiol.

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Temporomandibular joint osteoarthritis (TMJOA) is a common degenerative joint disease that can cause severe pain and dysfunction. It has a serious impact on the quality of lives of patients. Since mechanism underlying the pathogenesis of TMJOA is not fully understood, the development of effective tools for early diagnosis and disease-modifying therapies has been hindered. Animal models play a key role in understanding the pathological process of diseases and evaluating new therapeutic interventions. Although some similarities in disease processes between animals and humans are known, no one animal model is sufficient for studying all characteristics of TMJOA, as each model has different translatability to human clinical conditions. For the past 4 decades, TMJOA animal models have been studied by numerous researchers and can be broadly divided into induced, naturally occurring, and genetically modified models. The induced models can be divided into invasive models (intra-articular injection and surgical induction) or non-invasive models (mechanical loading, high-fat diet, and sleep deprivation). Different types of animal models simulate different pathological expressions of TMJOA and have their unique characteristics. Currently, mice, rats, and rabbits are commonly used in the study of TMJOA. This review sought to provide a general description of current experimental models of TMJOA and assist researchers in selecting the most appropriate models for different kinds of research.

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“…Anterior disc displacement (ADD) is commonly associated with the beginning of TMD and then it has been regarded as a common surgical model for TMD in animals. Xu et al 70 utilized adult rabbits to surgically induce ADD. Specifically, a 3 cm incision was made along the right zygomatic arch, and the zygomatic‐squamosal suture was exposed in the anesthetized rabbit.…”

Section: Animal Models Of Tmdmentioning

confidence: 99%

Animal Models of Temporomandibular Disorder

Xiang

Tao

Liao

et al. 2021

JPR

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Temporomandibular disorders (TMD) are a group of diseases in the oral and maxillofacial region that can manifest as acute or chronic persistent pain, affecting millions of people worldwide. Although hundreds of studies have explored mechanisms and treatments underlying TMD, multiple pathogenic factors and diverse clinical manifestations make it still poorly managed. Appropriate animal models are helpful to study the pathogenesis of TMD and explore effective treatment measures. At present, due to the high cost of obtaining large animals, rodents and rabbits are often used to prepare TMD animal models. Over the past decade, various animal models have been intensively developed to understand neurobiological and molecular mechanisms of TMD, and seek effective treatments. Although these models cannot carry out all clinical features, they are valuable in revealing the mechanisms of TMD and creating curative access. Currently, there are multitudinous animal models of TMD research. They can be constructed in different means and summarized into four ways according to the various causes and symptoms, including chemical induction (intra-articular injection of ovalbumin, collagenase, formalin, vascular endothelial growth factor, intramuscular injection of complete Freund's adjuvant, etc.), mechanical stress stimulation (passive mouth opening, change of chewing load), surgical operation (partial disc resection, joint disc perforation) and psychological stress induction. Here, we summarize and discuss different approaches of animal models for determining neurophysiological and mechanical mechanisms of TMD and assess their advantages and limitations, respectively.

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Expression of endoplasmic reticulum stress protein in rabbit condyle cartilage following anterior disk displacement

Abstract: These results indicate that the ER stress pathway is activated in ADD cartilage and might promote the development of TMD.

Cited by 8 publications

References 27 publications

Salubrinal alleviates cartilage degradation in a rabbit temporomandibular joint osteoarthritis model

Salubrinal alleviates cartilage degradation in a rabbit temporomandibular joint osteoarthritis model

Animal Models of Temporomandibular Joint Osteoarthritis: Classification and Selection

Animal Models of Temporomandibular Disorder

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