Expression of epithin in mouse preimplantation development: Its functional role in compaction

Khang, Inkoo; Sonn, Seongkeun; Park, June-Hee; Rhee, Kunsoo; Park, Dongeun; Kim, Kyungjin

doi:10.1016/j.ydbio.2005.02.026

Cited by 20 publications

(14 citation statements)

References 30 publications

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“…RNA interference (RNAi) is an established powerful experimental tool for analyzing gene function in mammalian cells and embryos [14][15][16][17]. Applying a similar approach, we used an siRNA strategy to explore the specific function of the SCF/cKit system in mouse pre-implantation embryonic development.…”

Section: Electronic Supplementary Materialsmentioning

confidence: 99%

Stem cell factor/c-Kit signaling in in vitro cultures supports early mouse embryonic development by accelerating proliferation via a mechanism involving Akt-downstream genes

Lim

Eum

Lee

et al. 2010

J Assist Reprod Genet

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Purpose Stem cell factor (SCF)/c-Kit regulates the proliferation and survival of germ cells or stem cells; however, little is known about the role of SCF/c-Kit in preimplantation embryo development. Methods Using exogenous SCF supplementation and c-Kit siRNA injection, we investigated the role and mechanism of SCF/c-Kit in pre-implantation mouse embryos.Results Addition of soluble SCF to the culture medium improved blastocyst formation. c-Kit gene silencing reduced the rate of blastocyst formation and delayed embryonic development. The number of proliferating cells in c-Kit gene-silenced blastocysts decreased, whereas the number of apoptotic cells in blastocysts obtained from both experimental and the control groups was not affected. RT-PCR, immunostaining and western blotting revealed that proliferation-related Akt downstream targets were substantially affected by c-Kit gene silencing. Conclusion SCF/c-Kit signaling through Akt downstream targets is likely involved in mediating the cleavage and proliferation of blastomeres during mouse pre-implantation embryogenesis.

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Section: Electronic Supplementary Materialsmentioning

confidence: 99%

Stem cell factor/c-Kit signaling in in vitro cultures supports early mouse embryonic development by accelerating proliferation via a mechanism involving Akt-downstream genes

Lim

Eum

Lee

et al. 2010

J Assist Reprod Genet

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“…-dependent Ecadherin-catenin complex (Vestweber and Kemler 1985;Johnson et al 1986) but also through the co-localising vezatin adhesion complex (Hyenne et al 2005), the Ca 2+ -independent immunoglobulin family nectin complex ) and the epithin membrane serine protease (Khang et al 2005). Close cell-cell adhesion at compaction is accompanied by restructuring of the membrane cytoskeleton involving nucleation and formation of actin filaments (Sun et al 2013) facilitated by the suppression in expression of the actin filament depolymerising protein, cofilin-1 (Ma et al 2009).…”

Section: +mentioning

confidence: 99%

Cell Signalling During Blastocyst Morphogenesis

Eckert

Velazquez

Fleming

2015

Advances in Experimental Medicine and Biology

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Blastocyst morphogenesis is prepared for even before fertilisation. Information stored within parental gametes can influence both maternal and embryonic gene expression programmes after egg activation at fertilisation. A complex network of intrinsic, cell-cell mediated and extrinsic, embryo-environment signalling mechanisms operates throughout cleavage, compaction and cavitation. These signalling events not only ensure developmental progression, cell differentiation and lineage allocation to inner cell mass (embryo proper) and trophectoderm (future extraembryonic lineages) but also provide a degree of developmental plasticity ensuring survival in prevailing conditions by adaptive responses. Indeed, many cellular functions including differentiation, metabolism, gene expression and gene expression regulation are subject to plasticity with short- or long-term consequences even into adult life. The interplay between intrinsic and extrinsic signals impacting on blastocyst morphogenesis is becoming clearer. This has been best studied in the mouse which will be the focus of this chapter but translational significance to human and domestic animal embryology will be a focus in future years.

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“…At this stage the cells establish gap junctions [10,11]. Compaction is mediated by E-cadherin and associated proteins [12][13][14][15][16][17]. A detailed description of the mechanisms driving compaction is tangential to this review but can be found in Chen et al [18].…”

Section: Forming Trophectodermmentioning

confidence: 99%

The States of Pluripotency: Pluripotent Lineage Development in the Embryo and in the Dish

Rathjen

2014

ISRN Stem Cells

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The pluripotent cell lineage of the embryo comprises a series of temporally and functionally distinct intermediary cell states, the epiblast precursor cell of the newly formed blastocyst, the epiblast population of the inner cell mass, and the early and late epiblast of the postimplantation embryo, referred to here as early and late primitive ectoderm. Pluripotent cell populations representative of the embryonic populations can be formed in culture. Although multiple pluripotent cell states are now recognised, little is known about the signals and pathways that progress cells from the epiblast precursor cell to the late primitive ectoderm in the embryo or in culture. The characterisation of cell states is most advanced in mouse where conditions for culturing distinct pluripotent cell states are well established and embryonic material is accessible. This review will focus on the pluripotent cell states present during embryonic development in the mouse and what is known of the mechanisms that regulate the progression of the lineage from the epiblast precursor cell and the ground state of pluripotency to the late primitive ectoderm present immediately prior to cell differentiation.

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Expression of epithin in mouse preimplantation development: Its functional role in compaction

Cited by 20 publications

References 30 publications

Stem cell factor/c-Kit signaling in in vitro cultures supports early mouse embryonic development by accelerating proliferation via a mechanism involving Akt-downstream genes

Stem cell factor/c-Kit signaling in in vitro cultures supports early mouse embryonic development by accelerating proliferation via a mechanism involving Akt-downstream genes

Cell Signalling During Blastocyst Morphogenesis

The States of Pluripotency: Pluripotent Lineage Development in the Embryo and in the Dish

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