Expression of estrogen receptor, progesterone receptor and KI 67 in epithelial ovarian tumors and their histopathological correlation

Bhagora, Rinku; Malik, Reeni; Trichal, V. K.

doi:10.17511/ijmrr.2017.i06.03

“…In our present study the total ER percentage positivity was 71.42% which was same as obtained by Cardilo et al 17 in 1998 and close to Pooja S Naik et al 6 in 2015. The present study shows that ER had highest expression in serous carcinomas (83.87%) which was comparable to studies done by Rinku Bhagora et al 2017 (85%), 13 Pooja S Naik et al 2015 (100%) 6 and Mahak Wadhwa et al (70.5%). 18 The second largest expression of ER was seen in endometroid carcinomas (50%) which was followed by mucinous carcinomas (33.33%) in the present study.…”

Section: Discussionsupporting

confidence: 91%

STUDY OF SURFACE EPITHELIAL OVARIAN MALIGNANCIES WITH REFERENCE TO THEIR HISTOLOGICAL GRADING AND ER & KI-67 IMMUNOREACTIVITY

Saurabh¹,

Satpathy²

2019

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BACKGROUNDWe wanted to evaluate the relation of histopathological grading of malignant surface epithelial ovarian neoplasms and evaluate ER and Ki -67 as prognostic markers to provide the clinician a better platform for therapeutic management. METHODSAll patients attending OPD and diagnosed as ovarian neoplasms were included in the study & were further subjected to histopathological & IHC studies. ER scoring was done by ALLRED scoring system (proportion score + intensity score), score 0-2 was taken as negative and 3-8 was taken as positive. The Ki 67 labelling index was expressed as 1+ when 5-25% of cell stained, 2+ when 25-50% cells stained. 3+ when 50-75% cells stained, 4+ when 75-100% cell stained. RESULTSTotal number of cases was 42. Maximum number of cases were between fifth and sixth decade of life. 31 cases (73.80%) were serous cystadenocarcinomas, 6 cases (14.29%) were mucinous cystadenocarcinomas and 4 cases (9.53%) were endometroid carcinoma. One case of clear cell carcinoma (2.38%) was also seen. Oestrogen receptor was positive in 26/31 cases (83.87%) in serous cystadenocarcinoma, 2/6 (33.33%) cases of mucinous cystadenocarcinoma and, 2/4 cases (50%) of endometroid carcinoma and the single case of clear cell carcinoma was negative for ER. The total number of cases showing ER positivity was 71.42%. 12 cases were Grade 1 carcinomas showing ER positivity 58.33% (7/11) cases and Ki67 percentage 36.88%, 22 cases Grade 2 carcinomas showing 100% ER positivity and Ki67 percentage 66.98%, 8 cases in Grade 3 carcinomas showing 62.50% ER positivity (5/8 cases) and Ki67 index 81.30%. CONCLUSIONSSerous cystadenocarcinoma was the most common of all malignant surface epithelial ovarian tumours. ER positivity was maximum in Grade 2 tumours showing that once a case is ER positive, recommendations are there for hormone therapy whatever be the degree of positivity. ER was maximally expressed in serous carcinomas followed by mucinous carcinoma followed by endometroid carcinomas. Ki67 labelling index increases with increasing grade of surface epithelial ovarian malignancies. KEYWORDS ER, Ki-67, Malignant Surface Epithelial Ovarian TumoursHOW TO CITE THIS ARTICLE: Saurabh S, Satpathy PK. Study of surface epithelial ovarian malignancies with reference to their histological grading and ER & Ki-67 immunoreactivity.

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“…Persistent efforts are being conducted to assess the tumor molecular markers like ki-67 (a proliferation marker), p53, estrogen receptors, progesterone receptors, and E-cadherin (E-cad) of EOC by immunohistochemical (IHC) analysis [11,12]. Substantial evidences have reflected that metabolic reprogramming contributes to survival of the cancer cells [13], but the relation with metastasis and recurrence in OvCa needs attention.…”

Section: Introductionmentioning

confidence: 99%

Understanding the Correlation between Metabolic Regulator SIRT1 and Exosomes with CA‐125 in Ovarian Cancer: A Clinicopathological Study

Roy

¹

,

Das

²

,

Vernekar

³

et al. 2022

BioMed Research International

3

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Background. Ovarian cancer (OvCa), the deadliest gynaecological malignancy, is associated with poor prognosis and high mortality rate. Ovarian cancer has been related with CA-125 and metabolic reprogramming by SIRT1 leading to metastasis with the involvement of exosomes. Methods. Clinicopathological data of OvCa patients were collected to perform the analysis. Patients’ samples were collected during surgery for immunohistochemistry and flow cytometric analysis of SIRT1, HIF-1α, exosomal markers (CD81 and CD63), ki-67, and PAS staining for glycogen deposition. Adjacent normal and tumor tissues were collected as per the CA-125 levels. Results. CA-125, a vital diagnostic marker, has shown significant correlation with body mass index (BMI) ( P = 0.0153 ), tumor type ( P = 0.0029 ), ascites level, ascites malignancy, degree of dissemination, tumor differentiation, FIGO stage, TNM stage, laterality, and tumor size at P < 0.0001 . Since significant correlation was associated with BMI and degree of dissemination, as disclosed by IHC analysis, metabolic marker SIRT1 ( P = 0.0003 ), HIF-1α ( P < 0.0001 ), exosomal marker CD81 ( P < 0.0001 ), ki-67 status ( P = 0.0034 ), and glycogen deposition (P <0.0001) were expressed more in tumor tissues as compared to the normal ones. ROC analysis of CA-125 had shown 327.7 U/ml has the best cutoff point with 82.4% sensitivity and specificity of 52.3%. In addition, Kaplan-Meier plots of CA-125 ( P < 0.0001 ), BMI ( P = 0.001 ), degree of dissemination ( P < 0.0001 ), and ascites level (P <0.0001) reflected significant correlation with overall survival (OS). Upon multivariate Cox-regression analysis for overall survival (OS), BMI ( P = 0.008 , HR 1.759, 95% CI 1.156-2.677), ascites malignancy ( P = 0.032 , HR 0.336, 95% CI 0.124-0.911), and degree of dissemination ( P = 0.004 , HR 1.994, 95% CI 1.251-3.178) were significant proving to be independent indicators of the disease. Conclusion. Clinicopathological parameters like BMI, degree of dissemination, and ascites level along with CA-125 can be prognostic factors for the disease. Levels of CA-125 can depict the metabolic and metastatic factors. Thus, by targeting SIRT1 and assessing exosomal concentrations to overcome metastasis and glycogen deposition, individualized treatment strategy could be designed. In-depth studies are still required.

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Evaluation of estrogen receptor, progesterone receptor, and Ki 67 immunoexpression in epithelial ovarian tumors with histopathological correlation

Priyadarshini,

Patro,

Dash

et al. 2023

MGMJ

1

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Background: Ovarian cancer ranks sixth among women’s cancers globally and seventh among cancer-related deaths. Around 90% of ovarian cancers have an epithelial genesis. Steroid hormones like progesterone and estrogen largely influence the development of ovarian cancers. In ovarian neoplasms, the proliferation marker Ki 67 helps forecast the course of the disease. Objective: To study the immunohistochemical expression pattern of estrogen receptor (ER), progesterone receptor (PR), and Ki 67 in the surface epithelial ovarian tumors (SEOTs) and correlate the findings with the standard histopathological. Materials and Methods: This prospective study was conducted in the Department of Pathology, MKCG Medical College and Hospital, Brahmapur, from November 2020 to October 2022 on 62 surface epithelial ovarian tumor cases. All the patients were evaluated for standard histopathological parameters and immuno-histochemistry (IHC) status using ER, PR, and Ki 67 as primary antibodies. Results: A total of 62 cases of SEOTs constituted the study group, of which 42 (67.74%) were benign, 5 (8.06%) were borderline, and 15 (24.2%) were malignant on histopathological evaluation: serous SEOTs (41, 66.12%), mucinous SEOTs (18, 29.03%), endometrioid SEOTs (2, 3.22%), and clear cell carcinoma (1, 1.61%). ER expression was more in malignant tumors (10/15; 66.66%) than in borderline (3/5; 60%) and benign (11/42; 26.19%), but the expression of PR was more in benign (27/42; 64.28%), followed by borderline (3/5; 60%) and malignant (8/15; 53.33%) SEOTs. Expression of Ki 67 was found to be more malignant (14/15; 93.33%) than borderline (4/5; 80%) and benign (2/42; 4.76%) SEOTs. Conclusion: ER and Ki 67 were expressed more in malignant, followed by borderline and benign, whereas PR expression was more innocent than borderline and malignant.

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Expression of estrogen receptor, progesterone receptor and KI 67 in epithelial ovarian tumors and their histopathological correlation

Cited by 3 publications

References 12 publications

STUDY OF SURFACE EPITHELIAL OVARIAN MALIGNANCIES WITH REFERENCE TO THEIR HISTOLOGICAL GRADING AND ER & KI-67 IMMUNOREACTIVITY

STUDY OF SURFACE EPITHELIAL OVARIAN MALIGNANCIES WITH REFERENCE TO THEIR HISTOLOGICAL GRADING AND ER & KI-67 IMMUNOREACTIVITY

Understanding the Correlation between Metabolic Regulator SIRT1 and Exosomes with CA‐125 in Ovarian Cancer: A Clinicopathological Study

Evaluation of estrogen receptor, progesterone receptor, and Ki 67 immunoexpression in epithelial ovarian tumors with histopathological correlation

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