Expression of ezrin in oral squamous cell carcinoma: Prognostic impact and clinicopathological correlations

Safi, Ali-Farid; Nickenig, Hans-Joachim; Rothamel, Daniel; Zirk, Matthias; Thiele, Oliver C.; Grandoch, Andrea; Scheer, Martin; Zinser, Max; Zöller, Joachim E.; Drebber, Uta; Kreppel, Matthias

doi:10.1016/j.jcms.2015.08.011

Cited by 10 publications

(6 citation statements)

References 33 publications

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“…Increasing researches focus on the role of Ezrin in cancer invasion and metastasis since its initial identification. Overexpression of Ezrin is an independent prognostic factor with poor overall survival (OS) and disease-free survival (DFS) in pancreatic ductal adenocarcinomas [14], breast cancer [15] and oral squamous cell carcinoma (OSCC) [16], indicating the tumorpromotor function of Ezrin. Additionally, the expression and phosphorylation of Ezrin play a novel role in the apoptosis and proliferation of multiple human cancer cell lines [17][18][19].…”

Section: Original Papermentioning

confidence: 99%

Baicalein Inhibits Proliferation Activity of Human Colorectal Cancer Cells HCT116 Through Downregulation of Ezrin

Chen

Hou

Gao

et al. 2018

Cell Physiol Biochem

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Background/Aims: The present study was aimed at examining Ezrin expression in human colorectal cancer (CRC) tissues and elucidating the influence of baicalein on the proliferation of HCT116 cells. Methods: The expression of Ezrin was determined by qRT-PCR and immunohistochemistry. HCT116 cells were divided into four groups－ baicalein groups with various concentrations, pcDNA3.1-Ezrin group, si-Ezrin group and dual inhibitory group (baicalein + si-Ezrin). CCK-8 assay and flow cytometry (FCM) were employed to assess cell proliferation and to detect the distribution of cell cycle respectively. The expression levels of Ezrin protein and cell cycle-associated proteins were detected by using western blot. The proliferation ability of CRC cells was also evaluated in vivo. Results: Ezrin expression in CRC tissues was observably higher than that in adjacent colorectal tissues. With drug concentration and action time of baicalein increasing, the cell propagation capacity of HCT116 cells was decreased and the cell cycle progression was arrested. Ezrin expression was inhibited by the administration of baicalein in a dose-dependent way. The levels of CyclinD1 and CDK4 were also significantly decreased, but the expression of P53 pathway proteins P53 and P21 was markedly upregulated. Conclusion: Baicalein repressed proliferation of human colorectal cancer cells HCT116 and blocked cell cycle through downregulating Ezrin and upregulating P53 pathway-related proteins.

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Section: Original Papermentioning

confidence: 99%

Baicalein Inhibits Proliferation Activity of Human Colorectal Cancer Cells HCT116 Through Downregulation of Ezrin

Chen

Hou

Gao

et al. 2018

Cell Physiol Biochem

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“…[ 19 ] However, the different biological growth patterns of malignancies mean that the identification of and socio-demographic and clinicopathological risk factors is still of utmost importance for predicting mortality. [ 20 , 21 ] However, data on socio-demographic and clinicopathological characteristics of patients with OSCC are still rare and therefore, for clarification, need to be obtained for a larger numbers of patients. [ 22 ] Therefore, the present retrospective, single-center study investigated the importance of socio-demographic and clinicopathological parameters that influence the mortality rate of the patients suffering from OSCC in the Kelantanse population.…”

Section: Introductionmentioning

confidence: 99%

“…In recent years, the importance of biological markers for prognosis of patients with OSCC has been demonstrated [19] . However, the different biological growth patterns of malignancies mean that the identification of and socio-demographic and clinicopathological risk factors is still of utmost importance for predicting mortality [20,21] . However, data on socio-demographic and clinicopathological characteristics of patients with OSCC are still rare and therefore, for clarification, need to be obtained for a larger numbers of patients [22] .…”

Section: Introductionmentioning

confidence: 99%

Risk factors associated with the mortality rate of oral squamous cell carcinoma patients

Ahmad

Nawaz²,

Qurban³

et al. 2021

Medicine

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In Malaysia, oral cancer is very common and the reported 5-year survival of such patients is nearly 50% after treatment with surgery and radiotherapy, much lower than most of the developed countries. This study aimed to investigate the socio-demographic and clinicopathological parameters that influence the mortality rate of the patients suffering from oral squamous cell carcinoma (OSCC) in the Kelantanse population. In this retrospective study, data regarding socio-demographic, clinicopathological factors, and treatment outcome associated with OSCC were gathered from the archives of the medical records office of Hospital Universiti Sains Malaysia. For statistical analysis, simple and multiple logistic regression were performed. The significance level was set to P < .25. A total of 211 OSCC cases were registered in Hospital Universiti Sains Malaysia from January 1, 2000 to December 31, 2018. Majority of them were male (57.82%), non-smoker (54.97%), non-alcohol consumer (91.94%), and non-betel quid chewer (93.83%) Malay (60.66%) patients. The tongue was the most commonly involved part of the oral cavity (41.52%). Histologically, the majority of the cases had moderately-differentiated OSCC (52.82%). Most of the patients were diagnosed at stage IV at the time of diagnosis (61.61%). When this study was performed, the survival status of the majority of the patients was alive (68.24%). Within the analyzed socio-demographic and clinicopathological parameters, gender, alcohol consumption, T-classification, histological grading, and treatment status have been demonstrated as an independent risk factors for mortality rate in multivariate analysis. Hence, these parameters need to be taken into account for the individualized therapy management of OSCC patients.

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“…Additionally, we also verified the similar results in HCS-2 cells. Many studies in human clinical SCC tissues have revealed that PD-L1, ezrin, and moesin are widely expressed in laryngeal [41][42][43] and oral [44][45][46][47][48][49] SCCs, PD-L1; and that ezrin are expressed in tongue [50][51][52] and esophageal [53][54][55][56][57] SCCs; and that PD-L1 and moesin are expressed in uterine cervix SCC [58][59][60]. In contrast, radixin expression has been reported only in the human tongue SCC cell line Tca8113 [61].…”

Section: Discussionmentioning

confidence: 99%

Moesin Serves as Scaffold Protein for PD-L1 in Human Uterine Cervical Squamous Carcinoma Cells

Doukuni

Kobori

Tanaka

et al. 2022

JCM

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Immune checkpoint blockade (ICB) therapy targeting the programmed death ligand-1 (PD-L1)/PD-1 axis has emerged as a promising treatment for uterine cervical cancer; however, only a small subset of patients with uterine cervical squamous cell carcinoma (SCC) derives clinical benefit from ICB therapies. Thus, there is an urgent unmet medical need for novel therapeutic strategies to block the PD-L1/PD-1 axis in patients with uterine cervical SCC. Here, we investigated the involvement of ezrin/radixin/moesin (ERM) family scaffold proteins, which crosslink several plasma membrane proteins with the actin cytoskeleton, on the plasma membrane localization of PD-L1 in BOKU and HCS-2 cells derived from human uterine cervical SCC. Immunofluorescence analysis showed that PD-L1 colocalized with all three ERM proteins in the plasma membrane. Gene knockdown of moesin, but not ezrin and radixin, substantially reduced the plasma membrane expression of PD-L1, with limited effect on mRNA expression. An immunoprecipitation assay demonstrated the molecular interaction between PD-L1 and moesin. Moreover, phosphorylated, i.e., activated, moesin was highly colocalized with PD-L1 in the plasma membrane. In conclusion, moesin may be a scaffold protein responsible for the plasma membrane expression of PD-L1 in human uterine cervical SCC.

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Expression of ezrin in oral squamous cell carcinoma: Prognostic impact and clinicopathological correlations

Cited by 10 publications

References 33 publications

Baicalein Inhibits Proliferation Activity of Human Colorectal Cancer Cells HCT116 Through Downregulation of Ezrin

Baicalein Inhibits Proliferation Activity of Human Colorectal Cancer Cells HCT116 Through Downregulation of Ezrin

Risk factors associated with the mortality rate of oral squamous cell carcinoma patients

Moesin Serves as Scaffold Protein for PD-L1 in Human Uterine Cervical Squamous Carcinoma Cells

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