Expression of Feline Leukaemia Virus Antigens on Cat Lymphoma Cells: Kinetics of Biosynthesis

O’Brien, Stephen J.; Boone, Charles W.

doi:10.1099/0022-1317-35-3-511

Cited by 4 publications

(1 citation statement)

References 22 publications

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“…The FL-74 parent is productively infected with FL-74 strain of FeLV. Neither of the parental species will support replication of the alternative parent's virus (Table II and * Criteria of type C virus production include: particulate RDDP activity in cell supernates, budding particles on examination in transmission electron microscopy, and viral antigens (p30, gp70) on infected cell surfaces (14,36). * FL-74 is a lymphoid suspension cell which was a selective parameter used in hybrid production (13,27,36).…”

Section: Lododeoxyuridine (Idu) Induction Of N-tropic and Xenotropic mentioning

confidence: 99%

Bvr-1, a restriction locus of a type C RNA virus in the feline cellular genome: pleiotropic restriction of endogenous BALB virus in cat X mouse somatic cell hybrids.

O’Brien¹,

Simonson²

1978

The Journal of Experimental Medicine

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Bvr-1 is a dominant X-linked feline gene which restricts the replication of B-tropic murineleukemia virus (B-MuLV) in somatic cell hybrids between murine BALB/c-RAG cells and FL-74 feline cells. Since the hybrids were originally derived by the hypoxanthine aminopterin thymidine selection scheme, counter selection experiments on 6-thioguanine result in preferential survival of hybrid cells which have spontaneously lost the feline X-chromosome on which is located the structural gene for hypoxanthine guanine phosphoribosyl transferase (IMP: pyrophosphate phosphoribosyl transferase, E.C. 2.4.2.8) and Bvr-1. Back selected Bvr-1- cells express high parental levels of B-MuLV. Bvr-1 effectively restricts the IdU-mediated induction of the endogenous xenotropic BALB virus (BALB: virus 2) but not the endogenous N-tropic virus (BALB: virus 1). Pleiotropic restriction of B-MuLV and X-MuLV, but not N-MuLV suggests that the viral targets of Bvr-1 (either viral components or functions in viral assembly) of the B-tropic and X-tropic endogenous BALB viruses are similar to each other but distinct from the target in the N-tropic virus. Very low levels of B-MuLV are detected in restricted cells, but this residual virus is not infectious in either NIH-3T3 or BALB-3T3 mouse cells which are genotypically Fv-1N/Fv-1N and Fv-1B/Fv-1B, respectively. Passage of residual virus through host cells without Fv-1 related restriction (SC-1) results in production of infectious B-MuLV indistinguishable from that produced by RAG parent cells.

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