Expression of Fimbriae and Hemagglutination Activity in <i>Shigella boydii</i>

Utsunomiya, Akiyoshi; Nakamura, Michio; Hamamoto, Ayami

doi:10.1111/j.1348-0421.2000.tb02529.x

Cited by 4 publications

(1 citation statement)

References 6 publications

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“…Binding of lectins to muramic acid and N-acetylmuramicacid, carbohydrates present in the bacterial cell wall, has been reported earlier [25] , [26] . Almost all microorganisms express surface-exposed carbohydrates which may be covalently bound, as in glycosylated teichoic acids to peptidoglycan, or non-covalently bound, as in capsular polysaccharides [27] . Every surface-exposed carbohydrate is a potential lectin-reactive site.…”

Section: Resultsmentioning

confidence: 99%

Isolation and Partial Characterisation of a Novel Lectin from Aegle marmelos Fruit and Its Effect on Adherence and Invasion of Shigellae to HT29 Cells

et al. 2011

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Lectins are a class of ubiquitous proteins/glycoproteins that are abundantly found in nature. Lectins have unique carbohydrate binding property and hence have been exploited as drugs against various infectious diseases. We have isolated one such novel lectin from the fruit pulp of Aegle marmelos. The isolated lectin was partially characterised and its effect against Shigella dysenteriae infection was evaluated. The isolated lectin was found to be a dimeric protein with N-acetylgalactosamine, mannose and sialic acid binding specificity. The effect of Aegle marmelos fruit lectin on the adherence of Shigella dysenteriae to human colonic epithelial cells (HT29 cells) was evaluated by Enzyme Linked Immune Sorbent Assay and invasion was analysed. The protective nature of the Aegle marmelos fruit lectin was assessed by analyzing apoptosis through dual staining method. Aegle marmelos fruit lectin significantly inhibited hemagglutination activity of Shigella and its minimum inhibitory concentration is 0.625 µg/well. Further, at this concentration lectin inhibited Shigella dysenteriae adherence and invasion of HT29 cells and protects the HT29 cells from Shigella dysenteriae induced apoptosis. To conclude, isolated lectin dimeric protein with N-acetylgalactosamine, Mannose and sialic acid binding specificity and inhibits adherence and invasion of Shigellae to HT29 cells thus, protects the host.

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Section: Resultsmentioning

confidence: 99%

Isolation and Partial Characterisation of a Novel Lectin from Aegle marmelos Fruit and Its Effect on Adherence and Invasion of Shigellae to HT29 Cells

et al. 2011

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Adhesins, Receptors, and Target Substrata Involved in the Adhesion of Pathogenic Bacteria to Host Cells and Tissues

2014

Bacterial Adhesion to Animal Cells and Tissues

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Hemagglutinating activity is directly correlated with colonization ability of shigellae in suckling mouse model

et al. 2012

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The aim of the present study was to explore a new approach based on the hemagglutination (HA) assay to understand the colonization ability of Shigella spp. To study colonization ability, an animal model of 4-day-old suckling mouse, was exploited. We characterized the HA activity of 48 Shigella strains, with erythrocytes collected from rabbit, guinea pig, chicken, and sheep. Only rabbit and guinea pig erythrocytes showed positive HA reactions in most of the cases. On the basis of HA pattern, 4 strains from each serogroup were selected for in vivo colonization studies. Our results showed a positive correlation between HA activity and colonization ability of the strains belonging to different serogroups (groups A, B, C, and D) of Shigella. In all 4 serogroups, high HA titer was associated with greater intestinal colonization.

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Expression of Fimbriae and Hemagglutination Activity in Shigella boydii

Cited by 4 publications

References 6 publications

Isolation and Partial Characterisation of a Novel Lectin from Aegle marmelos Fruit and Its Effect on Adherence and Invasion of Shigellae to HT29 Cells

Isolation and Partial Characterisation of a Novel Lectin from Aegle marmelos Fruit and Its Effect on Adherence and Invasion of Shigellae to HT29 Cells

Adhesins, Receptors, and Target Substrata Involved in the Adhesion of Pathogenic Bacteria to Host Cells and Tissues

Hemagglutinating activity is directly correlated with colonization ability of shigellae in suckling mouse model

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