Hemagglutinating activity is directly correlated with colonization ability of shigellae in suckling mouse model

Abstract

The aim of the present study was to explore a new approach based on the hemagglutination (HA) assay to understand the colonization ability of Shigella spp. To study colonization ability, an animal model of 4-day-old suckling mouse, was exploited. We characterized the HA activity of 48 Shigella strains, with erythrocytes collected from rabbit, guinea pig, chicken, and sheep. Only rabbit and guinea pig erythrocytes showed positive HA reactions in most of the cases. On the basis of HA pattern, 4 strains from each serogroup were selected for in vivo colonization studies. Our results showed a positive correlation between HA activity and colonization ability of the strains belonging to different serogroups (groups A, B, C, and D) of Shigella. In all 4 serogroups, high HA titer was associated with greater intestinal colonization.

Haemaglutination of Shigella dysenteriae subunit pili protein with anti-haemaglutination of S. dysenteriae subunit pili protein as a molecule adhesion in mouse enterocytes

“…Recently, Mitra et al (2012) have found that hemagglutinating activity is directly correlated with colonization ability of Shigellae in suckling mouse model.…”

Identification of Hemagglutinin Protein from Streptococcus pneumoniae Pili as a Vaccine Candidate by Proteomic Analysis

“…in a suckling mouse model, thus showing hemagglutination may be used as a reliable indicator of bacterial adherence [10] . Further, a hemagglutinin, 160 kDa surface protein of gram-positive Staphylococcus saprophyticus exhibited adhesion to the urothelial cell, so this protein could be characterized as an adhesion.…”

“…High hemagglutination ability is highly associated with high colonizing ability. Mitra et al [10] showed that hemagglutinating ability, and colonizing ability were correlated in Shigella spp. This correlation is also present with the fimbrial hemagglutinin protein of Bordetella pertussis which mediates the attachment of bacteria to the mouse respiratory tract.…”

Antibodies against Shigella flexneri adhesion molecule outer membrane protein (OMP) can cross-react with OMPs of some Shigella species

“…Mitra et al found that the colonization ability of Shigella spp. was directly correlated with the hemagglutination activity in a suckling mouse model [24]. A recent in vivo study by Hamong et al showed that a 49.6 kDa Helicobacter pylori hemagglutinin protein prevented damage to the stomach caused by homologous bacteria in mice [25].…”