Expression of furA is modulated by NtcA and strongly enhanced in heterocysts of Anabaena sp. PCC 7120

López-Gomollón, Sara; Hernández, José Ángel; Wölk, C. Peter; Peleato, M. Luisa; Fillat, Marı́a F.

doi:10.1099/mic.0.2006/000091-0

Cited by 44 publications

(40 citation statements)

References 49 publications

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“…Despite the identification of a new TSS, the consensus NtcA site remains 350 bp upstream of the new TSS. NtcA binding sites at distances greater than the prototypical distance of Ϫ40 bp have been demonstrated for several cyanobacterial genes, such as cox2, cox3, and furA (13,18).…”

Section: Discussionmentioning

confidence: 99%

Transcription of hupSL in Anabaena variabilis ATCC 29413 Is Regulated by NtcA and Not by Hydrogen

Weyman

Pratte

Thiel

2008

Appl Environ Microbiol

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Nitrogen-fixing cyanobacteria such as Anabaena variabilis ATCC 29413 use an uptake hydrogenase, encoded by hupSL, to recycle hydrogen gas that is produced as an obligate by-product of nitrogen fixation. The regulation of hupSL in A. variabilis is likely to differ from that of the closely related Anabaena sp. strain PCC 7120 because A. variabilis lacks the excision element-mediated regulation that characterizes hupSL regulation in strain PCC 7120. An analysis of the hupSL transcript in a nitrogenase mutant of A. variabilis that does not produce any detectable hydrogen indicated that neither nitrogen fixation nor hydrogen gas was required for the induction of hupSL. Furthermore, exogenous addition of hydrogen gas did not stimulate hupSL transcription. Transcriptional reporter constructs indicated that the accumulation of hupSL transcript after nitrogen stepdown was restricted primarily to the microaerobic heterocysts. Anoxic conditions were not sufficient to induce hupSL transcription. The induction of hupSL after nitrogen step-down was reduced in a mutant in the global nitrogen regulator NtcA, but was not reduced in a mutant unable to form heterocysts. A consensus NtcAbinding site was identified upstream of hupSL, and NtcA was found to bind to this region. Thus, while neither hydrogen gas nor anoxia controlled the expression of hupSL, its expression was controlled by NtcA. Heterocyst differentiation was not required for hupSL induction in response to nitrogen step-down, but heterocystlocalized cues may add an additional level of regulation to hupSL.

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Section: Discussionmentioning

confidence: 99%

Transcription of hupSL in Anabaena variabilis ATCC 29413 Is Regulated by NtcA and Not by Hydrogen

Weyman

Pratte

Thiel

2008

Appl Environ Microbiol

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“…PCC 7120, northern blot and western blot analyses showed an increase in furA expression and a corresponding decrease in alr1690-α-furA expression in nitrogen fixing conditions. Nitrogen deprivation upregulates the level of furA mRNA and the corresponding proteins, specifically in pro-heterocysts and mature heterocysts ( Lopez-Gomollon et al 2006). Nitrogen status has no effect on the transcription level or on the expression of FurB and FurC paralogues.…”

Section: Regulation Of Fur In Cyanobacteriamentioning

confidence: 99%

Ferric Uptake Regulator (FUR) protein: properties and implications in cyanobacteria

et al. 2015

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The Ferric uptake regulator (Fur) protein is a global iron regulator found in most prokaryotes. Although the Fur protein is involved in a variety of metabolic pathways, it is specifically known for the regulation of several iron responsive genes. It binds to the highly conserved sequences located in the upstream promoter region known as iron boxes, using ferrous ion as a co-repressor. Apart from that, the Fur protein is also directly/indirectly involved in a variety of other crucial physiological pathways. Hence, understanding the mechanism of action and the mechanistic pathways of iron regulation by Fur is necessary and important. The basic understanding of the functioning and properties of Fur protein along with its role, interaction and regulation at various levels in cyanobacteria has been discussed in detail.

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“…Besides, several iron-responsive genes in cyanobacteria, such as nblA, petH, pkn41, pkn42, among others, are also modulated by NtcA. [14][15][16] We have recently shown that NtcA modulates the expression of FurA, that is strongly enhanced in the heterocyst, 17 providing a very strong evidence of the link between those regulators.…”

Section: Introductionmentioning

confidence: 96%

Cross-talk Between Iron and Nitrogen Regulatory Networks in Anabaena (Nostoc) sp. PCC 7120: Identification of Overlapping Genes in FurA and NtcA Regulons

López-Gomollón

Hernández

Pellicer

et al. 2007

Journal of Molecular Biology

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Expression of furA is modulated by NtcA and strongly enhanced in heterocysts of Anabaena sp. PCC 7120

Cited by 44 publications

References 49 publications

Transcription of hupSL in Anabaena variabilis ATCC 29413 Is Regulated by NtcA and Not by Hydrogen

Transcription of hupSL in Anabaena variabilis ATCC 29413 Is Regulated by NtcA and Not by Hydrogen

Ferric Uptake Regulator (FUR) protein: properties and implications in cyanobacteria

Cross-talk Between Iron and Nitrogen Regulatory Networks in Anabaena (Nostoc) sp. PCC 7120: Identification of Overlapping Genes in FurA and NtcA Regulons

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