Expression of genes in cultivated rice and weedy rice in competition

Nohato, Marcos Andre; Benemann, Daiane de Pinho; Oliveira, C.; Várgas, Leandro; Agostinetto, Dirceu; Wheat, Embrapa

doi:10.21475/ajcs.2016.10.05.p7441

Cited by 4 publications

(3 citation statements)

References 34 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…This study shows that AOS induce differential expression of 13 and 22 photosynthesis-related genes in FL478 and IR29 under salt stress (Table S3 ). OsCAB1 [ 29 ] and the genes regulating LHCB proteins( OsLhcb2.1, OsLhcb6, OsLHCB4 ) [ 30 , 31 ] and OsLhcp [ 32 ] were involved in the regulation of light trapping in photosystem II. AOS may alleviate stress damage by modulating light-trapping proteins in rice seedlings exposed to salt stress.…”

Section: Discussionmentioning

confidence: 99%

Combined transcriptomic and metabolomic analysis of alginate oligosaccharides alleviating salt stress in rice seedlings

Du,

Liu,

Feng

et al. 2023

BMC Plant Biol

View full text Add to dashboard Cite

Background Salt stress is one of the key factors limiting rice production. Alginate oligosaccharides (AOS) enhance plant stress resistance. However, the molecular mechanism underlying salt tolerance in rice induced by AOS remains unclear. FL478, which is a salt-tolerant indica recombinant inbred line and IR29, a salt-sensitive rice cultivar, were used to comprehensively analyze the effects of AOS sprayed on leaves in terms of transcriptomic and metabolite profiles of rice seedlings under salt stress. Results In this experiment, exogenous application of AOS increased SOD, CAT and APX activities, as well as GSH and ASA levels to reduce the damage to leaf membrane, increased rice stem diameter, the number of root tips, aboveground and subterranean biomass, and improved rice salt tolerance. Comparative transcriptomic analyses showed that the regulation of AOS combined with salt treatment induced the differential expression of 305 and 1030 genes in FL478 and IR29. The expressed genes enriched in KEGG pathway analysis were associated with antioxidant levels, photosynthesis, cell wall synthesis, and signal transduction. The genes associated with light-trapping proteins and RLCK receptor cytoplasmic kinases, including CBA, LHCB, and Lhcp genes, were fregulated in response to salt stress. Treatment with AOS combined with salt induced the differential expression of 22 and 50 metabolites in FL478 and IR29. These metabolites were mainly related to the metabolism of amino and nucleotide sugars, tryptophan, histidine, and β -alanine. The abundance of metabolites associated with antioxidant activity, such as 6-hydroxymelatonin, wedelolactone and L-histidine increased significantly. Combined transcriptomic and metabolomic analyses revealed that dehydroascorbic acid in the glutathione and ascorbic acid cycles plays a vital role in salt tolerance mediated by AOS. Conclusion AOS activate signal transduction, regulate photosynthesis, cell wall formation, and multiple antioxidant pathways in response to salt stress. This study provides a molecular basis for the alleviation of salt stress-induced damage by AOS in rice.

show abstract

Section: Discussionmentioning

confidence: 99%

Combined transcriptomic and metabolomic analysis of alginate oligosaccharides alleviating salt stress in rice seedlings

Du,

Liu,

Feng

et al. 2023

BMC Plant Biol

View full text Add to dashboard Cite

show abstract

“…This study shows that alginate oligosaccharides induce differential expression of 13 and 22 photosynthesis-related genes in FL478 and IR29 under salt stress (Table S3). OsCAB1 [25]and the genes regulating LHCB proteins OsLhcb2.1 OsLhcb6 OsLHCB4 [26,27] and OsLhcp [28] were involved in the process of regulating light trapping in photosystem II. Alginate oligosaccharides may alleviate stress damage by modulating light-trapping proteins when the rice seedlings are experiencing salt stress.…”

Section: Discussionmentioning

confidence: 99%

Combined Transcriptome and Metabolome Analysis of Alginate Oligosaccharides Alleviating Salt Stress in the Rice Seedling Stage

Liu

Feng

et al. 2023

Preprint

View full text Add to dashboard Cite

Background: Salt stress is one of the main limiting factors of rice production. Alginate oligosaccharides can enhance plant stress resistance. However, the molecular mechanism of the benefits of alginate oligosaccharides on rice salt tolerance remains unclear. Rice FL478 and IR29 were used to comprehensively analyze the effects of leaf spraying alginate oligosaccharides on the transcriptomes and metabolites of rice seedlings under salt stress. Results: In this experiment, exogenous application of alginate oligosaccharides reduced the leaf membrane damage, increased the aboveground and below rice biomass, and improved rice salt tolerance. Comparative transcriptomic analyses showed that alginate oligosaccharides regulated the genes related to light-trapping proteins and RLCK receptor cytoplasmic kinases, including CBA, LHCB and Lhcp, in response to salt stress. In the metabolome profile study, among 22 and 50 different metabolites treated with alginate oligosaccharides + salt in FL478 and IR29, the abundance of metabolites with antioxidant activity, such as 6-Hydroxymelatonin, Wedelolactone and L-Histidine increased significantly. Combined transcriptome and metabolome analyses revealed that dehydroascorbic acid in the glutathione and ascorbic acid cycles plays a vital role in salt tolerance mediated by alginate oligosaccharides. Conclusion: Alginate oligosaccharides activate multiple pathways to cope with the adverse effects of salt stress. This study provides a molecular basis for alginate oligosaccharides alleviating salt stress damage in rice.

show abstract

“…Na área da ciência das plantas daninhas, inúmeros trabalhos são e podem ser realizados para avaliar a expressão de diversos genes. Uma vez que as plantas daninhas competem com as culturas pelos recursos do meio, através da quantificação do nível de expressão de determinados genes, é possível identificar quais são os genes responsivos ao estresse ocasionado pela competição (Nohato et al, 2016). Assim, o conhecimento da expressão dos genes envolvidos em cada processo pode levar a elaboração de estratégias de melhoramento de plantas visando maior habilidade competitiva frente as plantas daninhas, bem como ao incremento de produtividade nas diversas culturas.…”

Section: Expressão Gênicaunclassified

Biologia molecular aplicada à ciência das plantas daninhas

et al. 2018

View full text Add to dashboard Cite

Resumo -As plantas daninhas possuem elevada variabilidade genética, e principalmente por este motivo, são adaptadas a ambientes com intensa atividade humana. Embora o controle de plantas daninhas tenha evoluído de maneira positiva nos últimos anos, elas continuam a interferir na produção agrícola. O objetivo desta revisão bibliográfica é apresentar a contribuição da biologia molecular nos estudos aplicados a herbologia. Há lacunas entre o que aprendemos sobre genômica de plantas daninhas e como esses conhecimentos poderiam nos auxiliar no manejo e melhorar a competividade de culturas agrícolas frente às plantas daninhas. Muitos estudos na área da ciência das plantas daninhas podem ser realizados com o emprego de técnicas de biologia molecular, sendo eles: caracterização do genoma de espécies de plantas daninhas, visando à identificação destes com maior acurácia, identificação de espécies resistentes a herbicidas e seu mecanismo de resistência, variabilidade e similaridade genética entre populações de plantas daninhas, identificação de genes envolvidos nos processos de interação entre plantas, dentre outros. Palavras-chave: herbicidas, variabilidade genética, genômica, DNA, biotecnologia Abstract -The weeds have high genetic variability, mainly for this, they are adapted to environments disturbed by humans. Although weed control has evolved positively in recent years, they continue to interfere with agricultural production. The objective of this bibliographic review is to present the contribution of molecular biology in the studies applied to herbology. There area gap in what we learn about weed genomics, which could help us manage and improve the competitiveness of weed crops. Many studies in the field of weed science can be carried out using molecular biology techniques. The characterization of the genome of weed species, aiming to identify species with higher accuracy, identification of herbicide resistant species and its mechanism of resistance, genetic variability and similarity among weed populations, identification of genes involved in the interaction processes between plants, among other.

show abstract

Expression of genes in cultivated rice and weedy rice in competition

Cited by 4 publications

References 34 publications

Combined transcriptomic and metabolomic analysis of alginate oligosaccharides alleviating salt stress in rice seedlings

Combined transcriptomic and metabolomic analysis of alginate oligosaccharides alleviating salt stress in rice seedlings

Combined Transcriptome and Metabolome Analysis of Alginate Oligosaccharides Alleviating Salt Stress in the Rice Seedling Stage

Biologia molecular aplicada à ciência das plantas daninhas

Contact Info

Product

Resources

About