2016
DOI: 10.1016/j.ijpharm.2016.04.053
|View full text |Cite
|
Sign up to set email alerts
|

Expression of glutathione transferases in corneal cell lines, corneal tissues and a human cornea construct

Help me understand this report

Search citation statements

Order By: Relevance

Paper Sections

Select...
3
1
1

Citation Types

0
6
0

Year Published

2017
2017
2024
2024

Publication Types

Select...
7
1

Relationship

1
7

Authors

Journals

citations
Cited by 10 publications
(6 citation statements)
references
References 51 publications
0
6
0
Order By: Relevance
“…8A). 55 Out of all the genes analyzed, only three genes (3.6%) had more than 10-fold difference in expression compared to excised corneal epithelium. Glutathione S-transferases (GSTP1 and GSTM3), known to be involved in reactive oxygen species (ROS) detoxification, were significantly upregulated in 3D corneal tissues compared to excised corneal epithelium.…”
Section: Expression Of Drug-metabolizing Enzymesmentioning
confidence: 90%
See 1 more Smart Citation
“…8A). 55 Out of all the genes analyzed, only three genes (3.6%) had more than 10-fold difference in expression compared to excised corneal epithelium. Glutathione S-transferases (GSTP1 and GSTM3), known to be involved in reactive oxygen species (ROS) detoxification, were significantly upregulated in 3D corneal tissues compared to excised corneal epithelium.…”
Section: Expression Of Drug-metabolizing Enzymesmentioning
confidence: 90%
“…The two other genes, GSTP1 and GSTM3, are glutathione transferases that play an important role in the detoxification of carcinogens, therapeutic drugs, environmental toxins, and products of oxidative stress. 55 It is not clear if this deviation is an accurate representation of modified gene expression since its expression in excised corneal epithelium was detected at very low/low levels that may skew results reported as fold difference. Additional studies are needed to determine if these discrepancies are due to the donor variability or a result of modified gene expression in in vitro culture.…”
Section: Discussionmentioning
confidence: 99%
“…It is anticipated that a combination of geometrical cues at different scales can provide a powerful tool to guide cell behavior towards healthy wound healing and tissue regeneration. We finally note that, although the HCK cell line used in this study have been used as a faithful human cornea model, for instance in drug absorption and tissue engineering studies (Engelke et al, 2013;Grobe and Reichl, 2013;Kölln and Reichl, 2016;Türker et al, 2018), future clinical translations of our findings would require verification with primary cells and further animal studies.…”
Section: Discussionmentioning
confidence: 99%
“…GSTP1 is an enzyme involved in the detoxification of reactive oxygen species and its expression has been shown in corneal epithelial cells. 45 Increased expression of GSTP1 has been shown in tears of ADDE patients. 29 , 32 PGAM1 and PKM are enzymes involved in glycolysis.…”
Section: Discussionmentioning
confidence: 99%