Expression of growth factors in the mandibular distraction zone: a sheep study

Tavakoli, Kourosh; Yu, Youcheng; Shahidi, Shoaleh; Bonar, Fiona; Walsh, William R.; Poole, Michael D.

doi:10.1054/bjps.1999.3157

Cited by 49 publications

(44 citation statements)

References 27 publications

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“…However, similar to previously published reports (Tavakoli et al 1999;Yeung et al 2001), we have developed in our laboratory a special method to evaluate semiquantitatively our results, based on the percentage of cells showing a positive staining. Our results with this technique have been previously published for long bone (Rauch et al 2000;Hamdy et al 2003) and mandibular DO (Campisi et al 2003).…”

Section: Grading Of the Immunostained Sectionsmentioning

confidence: 78%

Immunohistochemical Localization of Bone Morphogenetic Protein-signaling Smads during Long-bone Distraction Osteogenesis

Haque¹,

Mandu-Hrit

Rauch

et al. 2006

J Histochem Cytochem.

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In this study we investigated the expression of bone morphogenetic protein (BMP)-signaling Smads in distraction osteogenesis (DO). Osteotomy of the right tibia was performed in 14 skeletally mature white New Zealand male rabbits. Lengthening was started 1 week later at a rate of 0.5 mm/12 hr and was maintained for 3 weeks. Expression of Smad proteins 1, 4, 5, 6, 7, and 8 and Smad ubiquitin regulatory factors (Smurfs) 1 and 2 was evaluated in the distracted zone using immunohistochemistry. Expression of receptor-regulated Smads (R-Smads) 1, 5, and 8 showed a significant increase during the distraction phase, followed by a gradual decrease during the consolidation phase. Smad 4 showed significant expression during both distraction and the beginning of the consolidation phase. Smad 6 and Smad 7 were highly expressed during the consolidation phase. Staining for both Smurfs 1 and 2 was maximal at the end of the distraction period. Staining for all proteins was most intense in chondrocyte and fibroblast-like cells. Expression pattern of R-Smads correlated with our previously reported expression pattern of BMPs 2, 4, and 7 and their receptors. These results therefore suggest a role for the whole BMP signaling pathway including the Smad proteins in DO.

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Section: Grading Of the Immunostained Sectionsmentioning

confidence: 78%

Immunohistochemical Localization of Bone Morphogenetic Protein-signaling Smads during Long-bone Distraction Osteogenesis

Haque¹,

Mandu-Hrit

Rauch

et al. 2006

J Histochem Cytochem.

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“…There is no clear evidence to guide us in terms of the timing of injection to achieve better bone regeneration. Several immunohistochemical analyses have shown high expression of growth factors receptors during the distraction phase [19,25,28,52,56,59]. In addition, Siwicka et al [52] showed rapid decline of the expression of the receptors for growth factors shortly after the termination of distraction.…”

Section: Discussionmentioning

confidence: 99%

Bone Marrow Aspirate Concentrate and Platelet-rich Plasma Enhanced Bone Healing in Distraction Osteogenesis of the Tibia

Lee¹,

Ryu²,

Kim³

et al. 2014

Clin Orthop Relat Res

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Background During lower limb lengthening, poor bone regeneration is a devastating complication. Several local or systemic applications have been used to promote osteogenesis, and biologic stimulations are gaining attention, but their utility has not been proven in this setting. Questions/purposes In patients undergoing bilateral tibial lengthening, we compared those receiving an osteotomy site injection of autologous bone marrow aspirate concentrate (BMAC) plus platelet-rich plasma (PRP) with those not receiving such an injection in terms of external fixator index (time in external fixation divided by amount of lengthening), full weightbearing index (time until a patient was permitted to do full weightbearing divided by amount of lengthening), four cortical healing indexes (time until each cortical union divided by amount of lengthening), and callus shape and type. Methods Twenty-two patients (44 tibias) undergoing bilateral tibial lengthening enrolled in this randomized trial. Two patients were excluded, one due to insufficient radiographic evaluation and one who was lost to followup, leaving 20 patients (40 segments) for inclusion. Ten patients (20 segments) received BMAC combined with PRP injection (treatment group) and 10 patients (20 segments) received no injection (control group). All patients underwent stature lengthening for familial short stature with the lengthening over nail technique. Autologous BMAC combined with PRP was injected at the tibial osteotomy site at the end of the index surgery. Mean distraction rates were similar between groups (0.75 mm/day in the treatment group versus 0.72 mm/day in the control group; p = 0.24). Full weightbearing was permitted when we observed radiographic evidence of healing at two cortices; this assessment was made by the surgeon who was blinded to the treatment each patient received. Minimum followup was 24 months (mean, 28 months; range, 24-34 months).

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“…Furthermore, semi-quantitative analysis of the immunohistochemically stained sections was performed as described previously [2,29,30]. The stained sections were analyzed under the light microscope by two experienced investigators with both investigators blinded to the staining.…”

Section: Preparation Of Biopsy Specimens For Histomorphometry and Immmentioning

confidence: 99%

Effect of a Particulate and a Putty-Like Tricalcium Phosphate-Based Bone Grafting Material on Bone Formation, Volume Stability and Osteogenic Marker Expression after Bilateral Sinus Floor Augmentation in Humans

Knabe

Adel‐Khattab

Kluk

et al. 2017

Preprint

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This study examines the effect of a hyaluronic acid (HyAc) containing tricalcium phosphate putty scaffold material (TCP-P) and of a particulate tricalcium phosphate (TCP-G) graft on bone formation, volume stability and osteogenic marker expression in biopsies sampled 6 months after bilateral sinus floor augmentation (SFA) in 7 patients applying a split-mouth design. Biopsies were processed for immunohistochemical analysis of resin embedded sections. Sections were stained for collagen type I (Col I), alkaline phosphatase (ALP), osteocalcin (OC) and bone sialoprotein (BSP). Furthermore, the bone area and particle area fraction were determined histomorphometrically. Cone-beam CT data recorded after SFA and 6 month later were used for calculating the graft volume at these two time points. TCP-P displayed more advantageous surgical handling properties and a significantly greater bone area fraction and smaller particle area fraction. This was accompanied by significantly greater expression of Col I and BSP and in osteoblasts and osteoid and a less pronounced reduction in grafting volume with TCP-P. SFA using both types of materials resulted in formation of sufficient bone volume for facilitating stable dental implant placement with all dental implants having been in function without any complications for 6 years. Since TCP-P displayed superior surgical handling properties and greater bone formation than TCP-G, without the Hyac hydrogel matrix having any adverse effect on bone formation or graft volume stability, TCP-P can be regarded as excellent grafting material for SFA in a clinical setting.

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Expression of growth factors in the mandibular distraction zone: a sheep study

Cited by 49 publications

References 27 publications

Immunohistochemical Localization of Bone Morphogenetic Protein-signaling Smads during Long-bone Distraction Osteogenesis

Immunohistochemical Localization of Bone Morphogenetic Protein-signaling Smads during Long-bone Distraction Osteogenesis

Bone Marrow Aspirate Concentrate and Platelet-rich Plasma Enhanced Bone Healing in Distraction Osteogenesis of the Tibia

Effect of a Particulate and a Putty-Like Tricalcium Phosphate-Based Bone Grafting Material on Bone Formation, Volume Stability and Osteogenic Marker Expression after Bilateral Sinus Floor Augmentation in Humans

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