Expression of growth hormone receptor by peripheral blood lymphocytes in children: evaluation in clinical conditions of impaired growth

Valerio, Giuliana; Bond, Heather M.; Badolato, Raffaele; Petrella, Anna; Maio, Salvatore Di; Salerno, M.; Waters, Michael J.; Venuta, Salvatore; Tenore, Alfred

doi:10.1046/j.1365-2265.1997.2571066.x

Cited by 17 publications

(8 citation statements)

References 28 publications

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“…Furthermore, mRNA expression of GHR, GHRtr, and their ratio showed no correlation with the donors' height SDS. This is consistent with previously reported results derived from peripheral blood lymphocytes [28]. The GHR/GHRtr ratio did not correlate with the absolute level of the two isoforms.…”

Section: Discussionsupporting

confidence: 93%

Relative Abundance of Growth Hormone Receptor Isoforms in Rhesus Monkey Tissues and Human Transformed Lymphocytes

Vottero

Kimchi-Sarfaty²,

Kratzsch³

et al. 2003

Horm Metab Res

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The growth hormone receptor (GHR) is expressed as one active, full-sequence isoform and one truncated, inactive one that lacks the intracellular signaling domain. The aim of this study was to investigate the variation in the tissue expression of the full and truncated mRNA and protein. Epstein-Barr virus-transformed human B lymphocyte lines were established from 9 normal individuals with a height standard deviation score (SDS) of - 0.1 +/- 1.1 (mean +/- SD). Tissues were also collected from 3 Rhesus monkeys, whose GHR has 94.1 % homology with the human molecule. mRNA quantitation was determined by Real Time Quantitative PCR. Growth hormone receptor expression in transformed lymphocytes was also studied by fluorescence-activated cell sorter analysis. Both isoforms were expressed in transformed lymphocytes, but individual variation in the relative mRNA expression was small (truncated isoform percentage of total receptor mRNA: 17.1 +/- 4.4, mean +/- SD). There was no correlation between donors' height SDS and the expression of either isoform or the ratio between them. Protein expression by FACS analysis showed wider variation among the subjects; however, the relative ratio was similar in all the subjects. In monkey tissues, the truncated receptor showed a tissue-specific distribution. In conclusion, the expression of both isoforms in transformed lymphocytes from normal subjects shows small differences at the RNA or protein levels, and does not correlate with height SDS. Growth hormone splice isoforms show tissue specificity, suggesting local regulation of splicing. Tissues with relatively high expression of the truncated isoform are likely to be more resistant to the effects of GH due to the dominant negative effect of this isoform. In addition, the differential tissue expression might influence the levels of growth hormone binding protein in the immediate milieu of each tissue.

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Section: Discussionsupporting

confidence: 93%

Relative Abundance of Growth Hormone Receptor Isoforms in Rhesus Monkey Tissues and Human Transformed Lymphocytes

Vottero

Kimchi-Sarfaty²,

Kratzsch³

et al. 2003

Horm Metab Res

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“…Aberrant GHR function may manifest as other traits related to GHR function in addition to prostate cancer risk. Both studies on mice and humans have reported changes in body mass and size with changes in the expressions of growth hormone and growth hormone receptor (13,(30)(31)(32), and genetic variation in other members of the growth hormone pathway have been implicated in BMI (33,34). We hypothesized that if the GHR haplotypes showing association with risk were truly involved in prostate cancer etiology, they may also bring changes in BMI (as it may be a surrogate marker for GHR expression levels).…”

Section: Discussionmentioning

confidence: 99%

Haplotype-Based Analysis of Common Variation in the Growth Hormone Receptor Gene and Prostate Cancer Risk

McKay

Kaaks

Johansson

et al. 2007

Cancer Epidemiology, Biomarkers &Amp; Prevention

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The growth hormone receptor (GHR) is potentially involved in prostate cancer through its role in stimulating insulin-like growth factor I production and its cellular effects on prostate epithelium. We have used a haplotype-based tagging approach within CAncer Prostate Sweden, a large retrospective case-control study of 2,863 cases and 1,737 controls to investigate if genetic variation in the GHR gene influences prostate cancer risk. One haplotype in the 3 ¶ region of the GHR gene was found associated with prostate cancer risk in elderly men (>65 years old at the time of diagnosis), with heterozygote haplotype carriers having an odds ratio of 1.65 (95% confidence interval, 1.21-2.16; P = 0.0009, P corrected = 0.03). GHR function has been implicated in the determination of body mass index. Interestingly, the same haplotype associated with risk in the 3 ¶ end of the GHR gene was also associated with a decrease in body mass index in controls (P = 0.003, P corrected = 0.05), possibly indicating some functionality with this haplotype. These results suggest that whereas genetic variation in the GHR gene does not seem to play a major role in prostate cancer etiology, one haplotype in the 3 ¶ region may be potentially relevant to cases with later onset of prostate cancer. (Cancer Epidemiol Biomarkers Prev 2007;16(1):169-73)

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“…Using flow cytometric analysis and immunofluorescence staining, GHR was shown to exist on B lymphocytes, T lymphocytes, natural killer cells and monocytes in human blood and the tonsil [10][11][12][13][14]. GHR expression was also shown in immune cells of other species: the PBMC, bone marrow, thymus, spleen and lymph node in rats [15][16][17] and bovine [18] (Table 1).…”

Section: Ghr In Immune Cellsmentioning

confidence: 99%

Expression, regulation and biological actions of growth hormone (GH) and ghrelin in the immune system

Hattori

2009

Growth Hormone & IGF Research

186

139

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Expression of growth hormone receptor by peripheral blood lymphocytes in children: evaluation in clinical conditions of impaired growth

Cited by 17 publications

References 28 publications

Relative Abundance of Growth Hormone Receptor Isoforms in Rhesus Monkey Tissues and Human Transformed Lymphocytes

Relative Abundance of Growth Hormone Receptor Isoforms in Rhesus Monkey Tissues and Human Transformed Lymphocytes

Haplotype-Based Analysis of Common Variation in the Growth Hormone Receptor Gene and Prostate Cancer Risk

Expression, regulation and biological actions of growth hormone (GH) and ghrelin in the immune system

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