Expression of hepatocyte growth factor in growing and regenerating rat skeletal muscle

Jennische, Eva; Ekberg, Staffan; Matejka, Göran

doi:10.1152/ajpcell.1993.265.1.c122

Cited by 115 publications

(83 citation statements)

References 14 publications

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“…Previous studies have reported that HGF is present in the extracellular matrix s u r r o u n d i n g m u s c l e f i b e r s 1 6 , 4 8 ) , o r immunolocalized at regenerating skeletal muscle 49) . In situ hybridization has also revealed HGF mRNA in regenerating muscle fibers 15,31) . In the present study, HGF was clearly immunolocalized in the cytoplasm of satellite cells early in the recovery process, strongly suggesting that HGF is produced by local satellite cells in response to reloading stimulation.…”

Section: Discussionmentioning

confidence: 99%

“…Previous studies have suggested that the proliferation of satellite cells is stimulated by growth factors such as hepatocyte growth factor (HGF) 15,16) , insulin-like growth factors [17][18][19] , fibroblast growth factors 20,21) , transforming growth factor β 10) , platelet-derived growth factor BB 10,14) , and epidermal growth factor 17,18) . However, only HGF has been shown to cause satellite cells to exit the G 0 phase and enter the G 1 phase in vitro 22,23) .…”

Section: Introductionmentioning

confidence: 99%

“…However, only HGF has been shown to cause satellite cells to exit the G 0 phase and enter the G 1 phase in vitro 22,23) . HGF was originally identified as a potent mitogen for hepatocytes 24) , and has previously been reported to be expressed in various organs, including the liver, lungs, kidneys, thymus and spleen 25) , and skeletal muscles 15,16) . The complete amino-acid sequence of human HGF was reported in 1989 26) .…”

Section: Introductionmentioning

confidence: 99%

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Hepatocyte Growth Factor in Mouse Soleus Muscle Increases with Reloading after Unloading

et al. 2006

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Abstract. Hepatocyte growth factor (HGF) has been suggested as a mitogen for skeletal muscle satellite cells and participates in skeletal muscle hypertrophy. The present study assessed HGF levels in mouse soleus and plantaris muscles during 14 days of tail suspension and 3 days of reloading using the enzymelinked immunosorbent assay. Immunohistochemical analyses were used to determine the locations of HGF, its receptor (c-Met) and proliferating cell nuclear antigen. In normal mice, HGF contents were 4.4 ± 0.5 ng/g tissue in the soleus muscle and 5.9 ± 1.2 ng/g tissue in the plantaris muscle, significantly higher than in the soleus muscle. HGF level in the soleus muscle was increased 314% from normal by reloading. HGF and c-Met were expressed in small cells contiguous to muscle fibers. Cells in similar positions displayed reactivity for PCNA, suggesting that these represent activated satellite cells. Thus, production of HGF protein appears to stimulated in satellite cells during recovery from disuse atrophy.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Hepatocyte Growth Factor in Mouse Soleus Muscle Increases with Reloading after Unloading

et al. 2006

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“…18 HGF mRNA and protein can be down-regulated by hypoxia, 39 although this may be a time-and tissue-dependent phenomenon. 15,40 In patients as well as in a myocardial ischemia model, c-met is up-regulated in response to hypoxia. 40 -42 Down-regulation of HGF in response to hypoxia may be an important contributor to the pathology associated with peripheral vascular disease, and the up-regulation of c-met in peripheral vascular disease may offer an opportunity for therapeutic intervention with HGF.…”

Section: Discussionmentioning

confidence: 99%

Hepatocyte Growth Factor Enhances Vascular Endothelial Growth Factor-Induced Angiogenesis in Vitro and in Vivo

Xin¹,

Yang²,

Ingle³

et al. 2001

The American Journal of Pathology

357

224

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Vascular endothelial growth factor (VEGF) is an important mediator of angiogenesis in both physiological and pathological processes. Hepatocyte growth factor (HGF) is a mesenchyme-derived mitogen that also stimulates cell migration, and branching and/or tubular morphogenesis of epithelial and endothelial cells. In the present study, we tested the hypothesis that simultaneous administration of HGF and VEGF would synergistically promote new blood vessel formation. HGF acted in concert with VEGF to promote human endothelial cell survival and tubulogenesis in 3-D type I collagen gels, a response that did not occur with either growth factor alone. The synergistic effects of VEGF and HGF on endothelial survival correlated with greatly augmented mRNA levels for the anti-apoptotic genes Bcl-2 and A1. Co-culture experiments with human neonatal dermal fibroblasts and human umbilical vein endothelial cells demonstrated that neonatal dermal fibroblasts, in combination with VEGF, stimulated human umbilical vein endothelial cells tubulogenesis through the paracrine secretion of HGF. Finally, in vivo experiments demonstrated that the combination of HGF and VEGF increased neovascularization in the rat corneal assay greater than either growth factor alone. We suggest that combination therapy using HGF and VEGF co-administration may provide a more effective strategy to achieve therapeutic angiogenesis.

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“…7). Increasing evidence has shown that HGF is a multifunctional polypeptide with actions on a wide variety of cell types, including microglia (8), skeletal muscle (9), Schwann cells (10), and central nervous system neurons (11)(12)(13).…”

mentioning

confidence: 99%

Hepatocyte Growth Factor Promotes Motor Neuron Survival and Synergizes with Ciliary Neurotrophic Factor

Wong

Glass

Arriaga

et al. 1997

Journal of Biological Chemistry

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Hepatocyte growth factor (HGF) has been shown to function as a potent mitogen for a variety of cells, transducing its signal through the c-met tyrosine kinase receptor. Ciliary neurotrophic factor (CNTF) is a cytokine that has been shown to promote survival of motor neurons. We show here that c-met mRNA is present in the embryonic rat spinal cord. Peak expression of c-met (at E14) coincides with the period of naturally occurring cell death in motor neurons, suggesting a possible role of HGF in the regulation of this process. Utilizing a neuron-enriched culture system, we established that HGF, like CNTF, stimulates choline acetyltransferase (CAT) activity in motor neurons. When co-administered to motor neuron cultures, saturating concentrations of HGF and CNTF produced a synergistic increase in CAT levels. We show that this synergy reflects enhanced motor neuron survival. Exposure of motor neuron cultures to the cytostatic agent vincristine markedly decreased CAT levels; co-treatment with HGF and CNTF (but not either factor alone) restored CAT activity to control levels. Our findings indicate that HGF is a survival factor for motor neurons, that it acts synergistically with CNTF, and that HGF and CNTF can together be neuroprotective in the face of vincristine toxicity.

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Expression of hepatocyte growth factor in growing and regenerating rat skeletal muscle

Cited by 115 publications

References 14 publications

Hepatocyte Growth Factor in Mouse Soleus Muscle Increases with Reloading after Unloading

Hepatocyte Growth Factor in Mouse Soleus Muscle Increases with Reloading after Unloading

Hepatocyte Growth Factor Enhances Vascular Endothelial Growth Factor-Induced Angiogenesis in Vitro and in Vivo

Hepatocyte Growth Factor Promotes Motor Neuron Survival and Synergizes with Ciliary Neurotrophic Factor

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