Expression of human protection of telomere 1 correlates with telomere length and radiosensitivity in the human laryngeal cancer Hep-2 cell line

Han, Lei; Feng, Deng; Zhou, Fuxiang; Xu, Hui; Tang, Tian; Yu, Haijun; Xie, Conghua; Zhou, Yunfeng

doi:10.3892/ol.2015.3332

Cited by 7 publications

(6 citation statements)

References 23 publications

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“…By investigating the relationship between TMPRSS4 and Telomere, we found that TMPRSS4 expression down-regulation reduce the telomere integrity via reduction of expression of POT1, TPP1 as the kind of telosome proteins, and up-regulation of UBE2D3 while TMPRSS4 overexpression reinforces its integrity. As it has been reported in our previous studies that UBE2D3 is negatively correlated with cell proliferation and telomere maintenance (Wang et al, 2013;Yang et al, 2016) and cell proliferation has been inhibited by affecting telomere integrity via silencing of POT1 (Lei et al, 2015;) and TPP1 (Yang et al, 2013), the present study suggest that TMPRSS4 modulation modulates cell proliferation by modulating telomere maintenance in breast cancer cells.…”

Section: Discussionsupporting

confidence: 83%

Role of TMPRSS4 Modulation in Breast Cancer Cell Proliferation

Assani

Yessoufou

Xiong

et al. 2019

Asian Pac J Cancer Prev

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Background: TMPRSS4 is a novel Type II transmembrane serine protease found at the surface of the cells and is involved in the development and cancer progression. However, TMPRSS4 functions in breast cancer remain poor understand. The present study investigated the function of TMPRSS4 in the breast cancer cells and the potential mechanistic action underling. Materials and Methods: The lentiviral vectors causing TMPRSS4 down-regulation and over-expression were established and transfected in MDA-MB-468 and MCF-7 cells, respectively. By using the CCK-8 assay, cell proliferation was analyzed. Moreover, western blot was used to detect the expression of certain proteins related to cell apoptosis (Bax and Bcl2) signaling pathway and telomere maintenance (POT1, TPP1, and UBE2D3). Cell cycle and cell apoptosis were also analyzed by using the Flow cytometry analysis. TMPRSS4 expression was detected at the mRNA level and protein level by performing qPCR and western blot technique, respectively. Results: TMPRSS4 expression is inhibited in stable transfected MDA-MB-468-shTMPRSS4 cells compared to the control MDA-MB-468-NC and its expression is up-regulated in stable transfected MCF-7-TMPTSS4 compared to its control MCF-7-NC. Moreover, TMPRSS4 silencing in breast cancer reduces cells proliferation by promoting cell cycle arrest in G2/M phase, cell apoptosis, and telomere maintenance impairment while the TMPRSS4 overexpression increases cells proliferation through cell apoptosis reduction and telomere maintenance reinforcement associated with insignificant change in cell cycle progression. Conclusion: TMPRSS4 plays important roles in cancer progression and may be considered as a good therapeutic target for cancer gene therapy especially breast cancer.

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Section: Discussionsupporting

confidence: 83%

Role of TMPRSS4 Modulation in Breast Cancer Cell Proliferation

Assani

Yessoufou

Xiong

et al. 2019

Asian Pac J Cancer Prev

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“…MiR-503 might decrease the radioresistance of laryngeal cancer cells via the inhibition of WEE1 [ 26 ]. Expression of hPOT1 was reported to be correlated with telomere length and radiosensitivity of laryngeal cancer cells [ 27 ]. ALDH1 was shown to act as a predictor of radioresistance in laryngeal cancer [ 28 ].…”

Section: Discussionmentioning

confidence: 99%

Integrin β1 regulates the invasion and radioresistance of laryngeal cancer cells by targeting CD147

Xiao

Peng

et al. 2018

Cancer Cell Int

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BackgroundIncreased expression of integrin β1 has been reported to correlate with progression and therapy resistance in many types of cancers. The aim of this study was to investigate the effects of integrin β1 on the invasion and radioresistance of laryngeal cancer cells.MethodsThe expression of integrin β1 in the tumor specimens of laryngeal cancer patients was assessed by immunohistochemical assays. The invasion ability of laryngeal cancer cells was detected by transwell and wound healing assays. The radiosensitivity of laryngeal cancer cells was evaluated by flow cytometry and colony formation assays.ResultsHigh expression of integrin β1 was significantly associated with lymph node metastasis, TNM stage and poor clinical outcomes (all p < 0.05). Knockdown of integrin β1 in laryngeal cancer cells inhibited invasion and increased radiosensitivity. Mechanistically, these effects were caused by suppression of the downstream focal adhesion kinase (FAK)/cortactin pathway. In addition, integrin β1 could interact with CD147 and the antibody blockade of CD147 led to the deactivation of FAK/cortactin signaling. Further studies revealed that the interaction between integrin β1 and CD147 relied on intact lipid rafts. Disruption of lipid rafts by methyl beta cyclodextrin in laryngeal cancer cells was able to reverse integrin β1-mediated malignant phenotypes.ConclusionsIntegrin β1 has potential as a therapeutic target in prevention and treatment of laryngeal cancer.

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“…Some studies suggest that POT1 is overexpressed in radioresistant cell lines of diverse cancer types [ 134 , 135 , 136 , 137 , 138 ]. In these cells, POT1 knockdown results in decreased radioresistance [ 134 , 137 ]. It has been suggested that increased POT1 levels may identify glioblastomas that would benefit from carbon ion hadron therapy [ 139 ].…”

Section: Clinical Implications Of Pot1 Alterations In Cancermentioning

confidence: 99%

Role of POT1 in Human Cancer

Poulos

Reddel

2020

Cancers

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Telomere abnormalities facilitate cancer development by contributing to genomic instability and cellular immortalization. The Protection of Telomeres 1 (POT1) protein is an essential subunit of the shelterin telomere binding complex. It directly binds to single-stranded telomeric DNA, protecting chromosomal ends from an inappropriate DNA damage response, and plays a role in telomere length regulation. Alterations of POT1 have been detected in a range of cancers. Here, we review the biological functions of POT1, the prevalence of POT1 germline and somatic mutations across cancer predisposition syndromes and tumor types, and the dysregulation of POT1 expression in cancers. We propose a framework for understanding how POT1 abnormalities may contribute to oncogenesis in different cell types. Finally, we summarize the clinical implications of POT1 alterations in the germline and in cancer, and possible approaches for the development of targeted cancer therapies.

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Expression of human protection of telomere 1 correlates with telomere length and radiosensitivity in the human laryngeal cancer Hep-2 cell line

Cited by 7 publications

References 23 publications

Role of TMPRSS4 Modulation in Breast Cancer Cell Proliferation

Role of TMPRSS4 Modulation in Breast Cancer Cell Proliferation

Integrin β1 regulates the invasion and radioresistance of laryngeal cancer cells by targeting CD147

Role of POT1 in Human Cancer

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