Expression of<i>MsLEC1</i>Transgenes in Alfalfa Plants Causes Symbiotic Abnormalities

Brill, Laurence M.; Fujishige, Nancy A.; Hackworth, Cheryl A.; Hirsch, Ann M.

doi:10.1094/mpmi.2004.17.1.16

Cited by 6 publications

(5 citation statements)

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“…The pleiotropic effects of MsLEC1 knockdown were either due to off-target activity of the antisense constructs or to MsLEC1 ’s involvement in multiple, essential biochemical pathways. Nevertheless, the reduced expression of MsLEC1 significantly reduced the number of nitrogen-fixing nodules because of premature nodule senescence (Brill et al 2004 ). Southern blot analysis indicated that three copies of a seed-type lectin gene exist in the alfalfa genome (Brill 1997 ), one of which, MsLEC3 , is a pseudogene (W.M.…”

Section: Use Of Model Legumes To Study Lectins and Lectin-like Proteimentioning

confidence: 99%

Plant lectins: the ties that bind in root symbiosis and plant defense

2009

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Lectins are a diverse group of carbohydrate-binding proteins that are found within and associated with organisms from all kingdoms of life. Several different classes of plant lectins serve a diverse array of functions. The most prominent of these include participation in plant defense against predators and pathogens and involvement in symbiotic interactions between host plants and symbiotic microbes, including mycorrhizal fungi and nitrogen-fixing rhizobia. Extensive biological, biochemical, and molecular studies have shed light on the functions of plant lectins, and a plethora of uncharacterized lectin genes are being revealed at the genomic scale, suggesting unexplored and novel diversity in plant lectin structure and function. Integration of the results from these different types of research is beginning to yield a more detailed understanding of the function of lectins in symbiosis, defense, and plant biology in general.

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Section: Use Of Model Legumes To Study Lectins and Lectin-like Proteimentioning

confidence: 99%

Plant lectins: the ties that bind in root symbiosis and plant defense

2009

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“…One of the proposed functions of legume lectins is to determine the specificity of the rhizobia-legume symbiosis (Hirsh 1999). For this study, Brill et al (2004) obtained transgenic alfalfa plants that expressed the alfalfa lectin MsLEC1 and MsLEC2 in sense and antisense orientations. MsLEC1-antisense (LEC1AS) plants were stunted, exhibited hypernodulation phenotype, and developed not only abnormally large nodules but also numerous small nodules, both of which senesced prematurely.…”

Section: Alfalafa (Medicago Sativa)mentioning

confidence: 99%

Transformation of leguminous plants to study symbiotic interactions

Iantcheva

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2013

Int. J. Dev. Biol.

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Legume plants are important in agriculture because they represent an important source of protein for human and animal consumption. This high protein content results from their capacity to use atmospheric nitrogen for their nutrition as a consequence of their symbiotic interaction with rhizobia. Understanding this interaction at the molecular level is a prerequisite for its better use in agriculture and for the long term objective of its transfer to other crops. Agrobacterium-mediated transformation is a tool of choice for studying this interaction and for unraveling the function of the different genes discovered through classical genetic approaches. However, legume plants are often recalcitrant to regeneration and transformation. This paper describes the technology developments (regeneration, transformation, insertion mutagenesis) related to Agrobacterium transformations that were established in the legume plants, as well as different examples of the technology developments or gene discoveries resulting from these studies. KEY WORDS: legume plant, agrobacterium, rhizobium, transformation, symbiosis Legumes and their importanceAmongst the mineral nutrients required by plants, nitrogen is the most important but its availability is unfortunately low in most soils. The intensive use of industrial nitrogen fertilizers in modern agriculture in the last 50 years has allowed the development of very productive crops, but their intensive use also resulted in eutrophication of continental waters including some areas in Europe. There is thus a strong need to reduce application of chemical nitrogen fertilizers and improve alternative nitrogen inputs (Graham and Vance 2003;Ferguson et al., 2010) in agriculture. A key contribution of legumes to sustainable agriculture and nitrogen cycle is their ability to fix atmospheric nitrogen in most agricultural ecosystems. They form specialized organs, the root nodules, in association with specific soil bacteria called rhizobia (including the genera Azorhizobium, Allorhizobium, Bradyrhizobium, Mesorhizobium, Rhizobium and Sinorhizobium). In these organs the bacteria differentiate into a form called the bacteroids and catalyze the reduction of atmospheric nitrogen gas (N 2 ) into ammonia using the nitrogenase enzyme complex. This process is commonly referred as "symbiotic nitrogen Abbreviations used in this paper: AM, arbuscular mycorrhiza; N2, nitrogen gas; NF, Nod factor; TDZ, thidiazuron; T-DNA, agrobacterium tranfered DNA.feed, and vegetable oil. They represent the third largest group of angiosperms and are the second largest group of food and feed crops grown worldwide. These plants are cultivated on 12-15% of available arable land and are responsible for more than 25% of the world's primary crop production. Around 250 million tons of grain legumes are produced annually world-wide. The ability of legumes to enter into symbiosis with nitrogen-fixing rhizobia provides them with a unique advantage compared to other plant species. For this reason legumes are called "green manu...

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“…Special attention should be attributed to lectin receptor kinases and b-galactosidase, which also contain a lectin domain [13,14]. In some plants, for instance, alfalfa, was identified lectin gene MsLEC1 [15]. However, there are no current data on the properties of this protein.…”

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confidence: 99%

“…The glycoprotein is capable of interacting with the peribacteroid membrane and the nodule bacteria surface [81]. The investigation of the expression of MsLec1, a lectin gene of Medicago sativa, demostrated its relevance for the symbiosis formation [15].…”

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“…Lectins are carbohydrate proteins wide-spread in different organisms from viruses to people. They play an important role in the processes of intercellular and intracellular recognition [1,2,15,72]. The proteins with lectin domains, due to their highly specific interaction with carbohydrate determinants, are capable of deciphering the glycocode [96], and as a result the cell metabolism is changed [35,76].…”

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confidence: 99%

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