Expression of IL17A in endometrial carcinoma and effects of IL17A on biological behaviour in Ishikawa cells

Abstract

Objective A growing body of evidence suggests chronic inflammation triggers the process of endometrial carcinogenesis. Interleukin (IL) 17A is an important proinflammatory factor involved in the tumour angiogenesis processes of many solid tumours. This study aimed to characterize the function of IL17A in endometrioid-type endometrial carcinoma. Methods Levels of IL17A in human endometrial tissues were analysed by immunohistochemistry. In vitro proliferation and migration were analysed in Ishikawa cells treated with IL17A, using cell counting kit-8, wound healing and transwell assays. Western blots were used to analyse levels of oestrogen receptor (ER)α and ERβ proteins in Ishikawa cells treated with IL17A. Results IL17A levels were significantly higher in endometrial carcinoma tissues than in endometrial hyperplasic tissues. Significantly increased proliferation and migration was observed in Ishikawa cells treated with IL17A versus controls. Investigation of the molecular mechanism revealed that IL17A treatment upregulated the ERα/ERβ protein ratio in Ishikawa cells. Conclusions IL17A may be an important proinflammatory factor involved in promoting endometrial carcinogenesis.

The roles and clinical applications of interleukins in endometrial carcinoma

“…Ning et al (111) suggested that IL-17A may not promote the cell growth directly but could have a vital effect on HEC-1A cells proliferation mediated by CD68 + CD163 + macrophages. However, the latest study by Cheng et al (82) confirmed the promoting effect of IL-17A on Ishikawa cells proliferation. The discrepancy among these studies may attribute to the differentiation grade and hormone receptor expression of cancer cells.…”

“…However, the latest study by Cheng et al. ( 82 ) confirmed the promoting effect of IL-17A on Ishikawa cells proliferation. The discrepancy among these studies may attribute to the differentiation grade and hormone receptor expression of cancer cells.…”

“…And it was confirmed that IL-8 ( 119 ) and IL-8 signaling pathways ( 120 ) were of vital importance to EC metastasis. Besides, IL-17A likewise promoted the migration of EC cells ( 82 ).…”

“…IL-17 has been reported to participate in the oncogenesis and development of a broad spectrum of tumors. As for the expression of IL-17 in EC tissue (Table 3), studies (49,82) showed that the mRNA and protein levels of IL-17 was both higher in EC lesions than normal endometrium. For the IL-17 levels in serum, Brooks et al (44) reported no significant difference between EC patients and women without cancer, but this study has an obvious limitation of sample size that it only included 12 EC patients and 10 controls.…”

“…IL-17 has been reported to participate in the oncogenesis and development of a broad spectrum of tumors. As for the expression of IL-17 in EC tissue ( Table 3 ), studies ( 49 , 82 ) showed that the mRNA and protein levels of IL-17 was both higher in EC lesions than normal endometrium. For the IL-17 levels in serum, Brooks et al.…”