Expression of immune-related genes in parasite-infected Tilapia nilotica (Oreochromis niloticus) from Egypt and molecular characterization of the parasites

Shater, Abdullah F.; AlGabbani, Qwait; Mohammedsaleh, Zuhair M.; Saleh, Fayez M.; AbouLaila, Mahmoud; Noreldin, Ahmed E.; Raza, Sayed Haidar Abbas; Ullah, Hameed; Khan, Rajwali; Menshawy, Soad

doi:10.1016/j.genrep.2021.101451

Cited by 3 publications

(2 citation statements)

References 26 publications

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“…Consequently, TNF-α promotes the release of E-selectin and speci c CC and CXC chemokines from endothelial cells, thereby elucidating the recruitment and activation of phagocytes. Additionally, it has the ability to increase the expression of MHC class II and CD83 from endothelial cells (Roca et al, 2008;Shater et al 2022). Histopathological alternations, a signi cant in ammatory and circulatory response, and the presence of D. ardeae in the a icted sh tissues could be attributed to the host's reaction or the pressure-induced production of poisonous chemicals by the larvae.…”

Section: Discussionmentioning

confidence: 99%

Epidemiological, multidisciplinary analysis using molecular, morphological, and immunological approaches in the Diplostomasis infections in Oreochromis niloticus

Mahdy,

Attia,

Salem

et al. 2024

Preprint

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We aim to describe the epidemiological, multidisciplinary analysis using molecular, morphological, and immunological approaches in the Diplostomasis infections on Oreochromis niloticus in different localities in Egypt. This was a descriptive retrospective study that collected 470 Nile tilapia samples from 4 governorates, in Egypt during the period from January 2022 and December 2023. The variables studied were epidemiological, molecular, morphological, and immunological approaches. For the isolates D. ardeaeand D. spathaecum, entirely sequences of the ITS2 region genes and partial sequences of the cytochrome C oxidase subunit 1 (Cox1) "barcode" area was amplified. Cox1 partial sequences were utilized to analyze the haplotype diversity and potential host-associated structures of the most prevalent parasite species, as well as for the genetic determination of isolates. New primers were designed for the amplification of the investigated genes. Therefore, this study aimed to detect various pro-inflammatory cytokines in fish infected with Diplostomum MCs using an immunological approach. (IFN-γ, IL-2, IL-6, TNF-α, & TNF-β) in the infected muscles contain Diplostomum sp. using quantitative real-time PCR (qRT-PCR). In addition, histopathological alterations of EMCs induced Zenkers necrosis and mononuclear inflammatory cells of fish muscle bundles response with muscular inflammation of infected fishes. In Egypt, an epidemiological study of two Diplostomid trematodes either metacercarial (D. spathaceum) in the eye and encysted metacercarial (D.ardeae) infections in the muscles of O. niloticus fish from four geographically different areas. The first genetic characterizations and immunological approach of two Diplostomum spp.; D. spathaceum and D.ardeae were all deposited in GenBank under the accession numbers (OR763842.1) and (OR755782.1).

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Section: Discussionmentioning

confidence: 99%

Epidemiological, multidisciplinary analysis using molecular, morphological, and immunological approaches in the Diplostomasis infections in Oreochromis niloticus

Mahdy,

Attia,

Salem

et al. 2024

Preprint

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“…Interferon-γ (IFN-γ) is a multipotent cytokine that can enhance immunity by activating signal transduction pattern recognition receptors in the congenital and adaptive immune systems [25]. It can affect cell response by regulating the expression level of multiple genes, such as improving NK cell activity and macrophage lysosome activity and promoting antigen presentation [48]. Research shows that the addition of CS to the diet could not only promote GIFT tilapia growth [26,49] but also significantly increase the expression levels of IL-1β and IFN-γ in GIFT tilapia kidney and enhance immunity and disease resistance [50].…”

Section: Plos Onementioning

confidence: 99%

Effects of different water quality regulators on growth performance, immunologic function, and domestic water quality of GIFT tilapia

Wang,

Liu,

Xie

et al. 2023

PLoS ONE

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Water quality regulation is widely recognized as a highly effective strategy for disease prevention in the field of aquaculture, and it holds significant potential for the development of sustainable aquaculture. Herein, four water quality regulators, including potassium monopersulfate (KMPS), tetrakis hydroxymethyl phosphonium sulfate (THPS), bacillus subtilis (BS), and chitosan (CS), were added to the culture water of Oreochromis niloticus (GIFT tilapia) every seven days. Subsequently, the effects of these four water quality regulators on GIFT tilapia were comprehensively evaluated by measuring the water quality index of daily growth-related performance and immune indexes of GIFT tilapia. The findings indicated that implementing the four water quality regulators resulted in a decrease in the content of ammonia nitrogen, active phosphate, nitrite, total organic carbon (TOC), and chemical oxygen demand (COD) in the water. Additionally, these regulators were found to maintain dissolved oxygen (DO) levels and pH of the water effectively. Furthermore, using these regulators demonstrated positive effects on various physiological parameters of GIFT tilapia, including improvements in final body weight, weight gain rate (WGR), specific growth rate (SGR), condition factor (CF), feed conversion ratio (FCR), spleen index (SI), hepato-somatic index (HSI), immune cell count, the activity of antioxidant-related enzymes (Nitric oxide, NO and Superoxide dismutase, SOD), and mRNA expression levels of immunity-related factors (Tumor Necrosis Factor-alpha, TNF-α and Interleukin-1 beta, IL-1β) in the liver and spleen. Notably, the most significant improvements were observed in the groups treated with the BS and CS water quality regulators. Moreover, BS and CS groups exhibited significantly higher serum levels of albumin (ALB) and total protein (TP) (P < 0.05), whereas the other indicators showed no significant difference (P > 0.05) compared to the control group. However, the KMPS and THPS groups of GIFT tilapia exhibited significantly higher serum levels of aspartate aminotransferase (AST), alanine transaminase (ALT), creatinine (CRE) and blood urea nitrogen (BUN) (P < 0.05), whereas they exhibited significantly decreased HSI (P < 0.05). In addition, the partially pathological observations revealed the presence of cell vacuolation, nuclear shrinkage, and pyknosis within the liver. In conclusion, these four water quality regulators, mainly BS and CS, could improve the growth performance and immunity of GIFT tilapia to varying degrees by regulating the water quality and then further increasing the expression levels of immune-related factors or the activity of antioxidant-related enzymes of GIFT tilapia. On the contrary, the prolonged use of KMPS and THPS may gradually diminish their growth-enhancing properties and potentially hinder the growth of GIFT tilapia.

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Evaluation of Azadirachta indica leaf extracts efficacy against gill flukes parasites with a focus on oxidative stress, pathological changes, and immune gene response in infected Nile tilapia

Radwan,

Abbas,

Fares

et al. 2024

Aquacult Int

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Expression of immune-related genes in parasite-infected Tilapia nilotica (Oreochromis niloticus) from Egypt and molecular characterization of the parasites

Cited by 3 publications

References 26 publications

Epidemiological, multidisciplinary analysis using molecular, morphological, and immunological approaches in the Diplostomasis infections in Oreochromis niloticus

Epidemiological, multidisciplinary analysis using molecular, morphological, and immunological approaches in the Diplostomasis infections in Oreochromis niloticus

Effects of different water quality regulators on growth performance, immunologic function, and domestic water quality of GIFT tilapia

Evaluation of Azadirachta indica leaf extracts efficacy against gill flukes parasites with a focus on oxidative stress, pathological changes, and immune gene response in infected Nile tilapia

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