Expression of Inflammatory Cytokines in Pigs Experimentally Infected with Mycoplasma hyopneumoniae

Choi, Chang Sik; Kwon, Dongil; Jung, Kwonil; Ha, Yoon‐Cheol; Lee, Y.-H.; Kim, O.; Park, H.-K.; Kim, S.-H.; Hwang, K.-K.; Chae, C.

doi:10.1016/j.jcpa.2005.06.009

Cited by 43 publications

(25 citation statements)

References 23 publications

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“…Similarly to previous investigations, a large amount of cytokine proteins were induced by M. hyopneumoniae exposure in murine macrophage RAW264.7 cells Thanawongnuwech et al 2004). In vivo expression of IL-1, TNF-α and IL-6 was demonstrated in lungs of pigs experimentally infected with M. hyopneumoniae (Choi et al 2006). This could be helpful in triggering the cellular immune response; however, the large amount of proinflammatory cytokine observed could result in tissue damages and even death of the host (Thanawongnuwech et al 2004).…”

Section: Discussionsupporting

confidence: 72%

Mycoplasma hyopneumoniae induces pro-inflammatory cytokine and nitric oxide production through NFκB and MAPK pathways in RAW264.7 cells

et al. 2010

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In the present study, we confirmed the ability of M. hyopneumoniae to induce the secretion of large amount of proinflammatory cytokine and nitric oxide (NO) in murine macrophage RAW 264.7 cells. Moreover, M. hyopneumoniae-induced activation of the MAPK and NF-кB pathways by phosphorylation of ERK1/2, p38 and JNK/SAPK and by dissociation of IκB from NF-κB. Translocation of transcription factor NF-κB and its binding was confirmed through western blot and electromobility shift assay. From these results, we further hypothesized that these signal proteins were involved in M. hyopneumoniae-induced proinflammatory cytokines and NO productions in macrophages. Hence, we utilized specific blockers of MAPK and NF-κB to investigate the signaling pathway involvement in cytokine and NO production through pharmacological approaches. The results demonstrated significant inhibition of TNF-α, IL-1β, IL-6 and NO by MAPK inhibitors. NF-κB inhibitor PDTC significantly inhibited IL-1β and NO production. These findings contribute to the understanding of the mechanisms of immune reactivity and may ultimately prove useful in the development of new therapeutic strategies. In summary, we found critical evidence for the involvement of NF-κB and MAPK signaling pathways in the upregulation of proinflammatory cytokine and NO induced by M. hyopneumoniae.

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Section: Discussionsupporting

confidence: 72%

Mycoplasma hyopneumoniae induces pro-inflammatory cytokine and nitric oxide production through NFκB and MAPK pathways in RAW264.7 cells

et al. 2010

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“…However, an important role of IL-17 in recruitment of the neutrophilic leukocyte in the biophylaxis response to infection is pointed out (Wu et al 2007). TNF-α is an important proinflammatory cytokine in the protective and inflammatory responses in lungs after Mph infection (Kelley 1990;Asai et al 1993;Choi et al 1999Choi et al , 2006. IFN-γ is mainly produced by Th1-type T cells and mediates cellular immunity under Mhp infection, promoting the proliferation of macrophage to repair damaged cells (Rodríguez et al 2007).…”

Section: Genetic Trendsmentioning

confidence: 99%

“…These results suggested that cellular immunity is important compared to antibody-mediated immunity in Mhp resistance. TNF-α is an important proinflammatory cytokine in the protective and inflammatory responses in lungs after Mph infection (Kelley 1990;Asai et al 1993;Choi et al 1999Choi et al , 2006. These two cytokines control innate immunity, which may be more important in the defense against Mhp infection.…”

Section: Genetic Trendsmentioning

confidence: 99%

“…The breeding values of MPS and BF decreased with selection, and the ADG value increased. Cytokine activity is also important in animals infected with Mhp (Choi et al 2006;Lorenzo et al 2006). The breeding values of innate immunity (e.g.…”

Section: Introductionmentioning

confidence: 99%

“…Further, cytokines plays an important role to the control of infection defense, adjustment of biofunction and control of restraint of the onset of various diseases. Cytokine activity is also important in animals infected with Mhp (Choi et al 2006;Lorenzo et al 2006). Therefore, in the present work, we assessed genetic parameters and genetic trends in selection traits, correlated immunity traits and peripheral blood cytokine concentrations, to clarify the immunogenic properties of this Landrace population selected for decreased morbid MPS lesions.…”

Section: Introductionmentioning

confidence: 99%

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Correlated response of peripheral blood cytokines with selection for reduced mycoplasma pneumonia of swine lesions in Landrace pigs

Sato

Okamura

Kojima-Shibata³

et al. 2015

Animal Science Journal

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Mycoplasma pneumonia of swine (MPS) is responsible for significant economic losses in the swine industry. We selected Landrace pigs for reduced MPS pulmonary lesions over five generations, and measured concentrations of the following cytokines: interleukin (IL)-10, IL-13, IL-17, tumor necrosis factor (TNF)-α and interferon (IFN)-γ to estimate their correlation with MPS lesions. Sheep red blood cells (SRBC) were injected twice intramuscularly at 70 and 95 kg body weight. Blood serum samples were collected after 1 week of secondary SRBC inoculation and cytokine concentrations were analyzed by ELISA. Genetic parameters and breeding values were estimated. The heritability estimates of IL-10, IL-13, IL-17, TNF-α and IFN-γ were 0.20 ± 0.06, 0.12 ± 0.06, 0.27 ± 0.07, 0.20 ± 0.10 and 0.05 ± 0.03, respectively. Genetic correlations of IL-17 and TNF-α with pulmonary MPS lesions were high (-0.86 ± 0.13 and 0.69 ± 0.29, respectively) and those of IFN-γ and IL-13 with MPS lesions were moderately negative (-0.45). Through selection, the breeding values of IL-17 and IFN-γ increased substantially and those of TNF-α decreased. These results suggest that innate and cellular immunity are more important for the suppression of pulmonary lesions in MPS than humoral-mediated immunity, such as antibody response.

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Mapping of quantitative trait loci for mycoplasma and tetanus antibodies and interferon-gamma in a porcine F2 Duroc × Pietrain resource population

et al. 2010

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The aim of the present study was to detect quantitative trait loci (QTL) for innate and adaptive immunity in pigs. For this purpose, a Duroc x Pietrain F(2) resource population (DUPI) with 319 offspring was used to map QTL for the immune traits blood antibodies and interferon-gamma using 122 microsatellites covering all autosomes. Antibodies response to Mycoplasma hyopneumoniae and tetanus toxoid vaccine and the interferon-gamma (IFNG) serum concentration were measured at three different time points and were used as phenotypes. The differences of antibodies and interferon concentration between different time points were also used for the linkage mapping. Line-cross and imprinting QTL analysis, including two-QTL, were performed using QTL Express. A total of 30 QTL (12, 6, and 12 for mycoplasma, tetanus antibody, and IFNG, respectively) were identified at the 5% chromosome-wide-level significant, of which 28 were detected by line-cross and 2 by imprinting model. In addition, two QTL were identified on chromosome 5 using the two-QTL approach where both loci were in repulsion phase. Most QTL were detected on pig chromosomes 2, 5, 11, and 18. Antibodies were increased over time and immune traits were found to be affected by sex, litter size, parity, and month of birth. The results demonstrated that antibody and IFNG concentration are influenced by multiple chromosomal areas. The flanking markers of the QTL identified for IFNG on SSC5 did incorporate the position of the porcine IFNG gene. The detected QTL will allow further research in these QTL regions for candidate genes and their utilization in selection to improve the immune response and disease resistance in pig.

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Expression of Inflammatory Cytokines in Pigs Experimentally Infected with Mycoplasma hyopneumoniae

Cited by 43 publications

References 23 publications

Mycoplasma hyopneumoniae induces pro-inflammatory cytokine and nitric oxide production through NFκB and MAPK pathways in RAW264.7 cells

Mycoplasma hyopneumoniae induces pro-inflammatory cytokine and nitric oxide production through NFκB and MAPK pathways in RAW264.7 cells

Correlated response of peripheral blood cytokines with selection for reduced mycoplasma pneumonia of swine lesions in Landrace pigs

Mapping of quantitative trait loci for mycoplasma and tetanus antibodies and interferon-gamma in a porcine F2 Duroc × Pietrain resource population

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