Expression of intercellular adhesion molecule-1 (ICAM-1) and vascular cell adhesion molecule-1 (VCAM-1) on proliferating vascular endothelial cells in diabetic epiretinal membranes.

Tang, Shibo; Le-Ruppert, K C; Gabel, Virginie

doi:10.1136/bjo.78.5.370

Cited by 29 publications

(29 citation statements)

References 31 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…We believe that this finding cannot be attributed to the molecular size of VCAM-1, because serum diffusion is the primary reason for the intravitreous enhancement of a bigger molecule, such as IGF-I (19,20). Although our results do not suggest intraocular production of VCAM-1 in diabetic patients with PDR, there is relevant information arguing the contrary (7,8). To reconcile these opposing data, it is tempting to hypothesize that VCAM-1 is quenched by the retina in the passage from the blood stream to the vitreous fluid.…”

Section: Vcam-1 and Vegf In Diabetic Retinopathycontrasting

confidence: 53%

See 1 more Smart Citation

Vitreous Levels of Vascular Cell Adhesion Molecule and Vascular Endothelial Growth Factor in Patients With Proliferative Diabetic Retinopathy

Hernández¹,

Burgos²,

Cantón³

et al. 2001

Diabetes Care

View full text Add to dashboard Cite

OBJECTIVE -To evaluate the intravitreous concentration of vascular cell adhesion molecule (VCAM)-1 in diabetic patients with proliferative diabetic retinopathy (PDR) and the relationship of VCAM-1 with vascular endothelial growth factor (VEGF).RESEARCH DESIGN AND METHODS -Serum and vitreous fluid samples were obtained simultaneously at the onset of vitrectomy from 20 diabetic patients with PDR and 20 nondiabetic control subjects with nonproliferative ocular disease. Both groups were matched by serum levels of VCAM-1 and VEGF. VCAM-1 and VEGF were determined by enzyme-linked immunosorbent assay. Statistics were determined using the Mann-Whitney U test and Spearman's rank correlation test.RESULTS -The intravitreous concentration of VCAM-1 was significantly elevated in diabetic patients with PDR compared with control subjects (26 ng/ml [19 -118] vs. 22 ng/ml [20 -47], P Ͻ 0.05). A direct correlation between VCAM-1 and total vitreous proteins was detected in diabetic patients (r ϭ 0.64, P ϭ 0.003), but not in control subjects. After adjusting for total intravitreous proteins, VCAM-1 was significantly lower in diabetic patients with PDR than in control subjects (8.2 ng/ml [4 -31.4] vs. 43.1 ng/ml [9.7-100], P Ͻ 0.001). Intravitreous VEGF concentrations were higher in patients with PDR than in control subjects in absolute terms (1.34 ng/ml [0.16 -6.22] vs. 0.009 ng/ml [0.009 -0.044], P Ͻ 0.0001) and after correcting for total vitreal proteins (0.33 ng/ml [0.01-2.3] vs. 0.013 ng/ml [0.003-0.035], P ϭ 0.0001). Finally, the vitreous ratio of VCAM-1 to proteins correlated with the vitreous ratio of VEGF to proteins in both diabetic patients (r ϭ 0.74, P ϭ 0.001) and control subjects (r ϭ 0.84, P ϭ 0.005).CONCLUSIONS -The low proportion of VCAM-1 in relation to total vitreal proteins observed in diabetic patients with PDR suggests that VCAM-1 is quenched by diabetic retina. In addition, the direct correlation detected between VCAM-1 and VEGF suggests that cellular adhesion and neovascularization may be linked processes. Diabetes Care 24:516 -521, 2001P roliferative diabetic retinopathy (PDR) is characterized by extensive neovascularization and vessel intrusion into the vitreous body, with subsequent bleeding and scarring surrounding new vessels, leading to severe visual impairment. Ischemic areas of the retina seem to be essential stimuli for the angiogenic process mediated by specific growth factors. This includes the expression of vascular endothelial growth factor (VEGF) by retinal glial cells (1) and vascular endothelial cells (2). It is thought that capillary occlusion by increased adhesion of leukocytes and macrophages to the endothelium has a crucial role in the process of retinal ischemia (3). In addition, monocytes exposed to glycated collagen show increased adhesiveness (4), and several studies indicate that circulating leukocytes are activated in diabetic patients (5,6).Vascular cell adhesion molecule (VCAM)-1, a member of the immunoglobulin supergene family of cellular adhesion molecules, is involved in the re...

show abstract

Section: Vcam-1 and Vegf In Diabetic Retinopathycontrasting

confidence: 53%

“…Interestingly, the expression of VCAM-1 has been found in epiretinal membranes from diabetic patients with PDR (7,8). In addition, it has been demonstrated that VCAM-1 promotes angiogenesis both in vitro and in vivo (9,10), but its relationship with VEGF has not been reported.…”

mentioning

confidence: 96%

Vitreous Levels of Vascular Cell Adhesion Molecule and Vascular Endothelial Growth Factor in Patients With Proliferative Diabetic Retinopathy

Hernández¹,

Burgos²,

Cantón³

et al. 2001

Diabetes Care

View full text Add to dashboard Cite

show abstract

“…Therefore, increased ICAM-1 levels have been suggested to play a crucial role in the development of DRP. VCAM-1 is also an adhesion molecule that mediates leukocyte adhesion and is considered to play an important role in the development of DRP (25). ET-1 has been demonstrated to play a role in the development of DRP in diabetic animals (13).…”

Section: Discussionmentioning

confidence: 99%

“…The test was performed according to the guidelines defined by Corretti et al (24) in a quiet room at a temperature of [22][23][24][25] in the morning (between 8:00-9:00 AM) after 12 hours of fasting. The use of alcohol, caffeine or vasoactive drugs and smoking were restricted during the fasting period.…”

Section: Evaluation Of the Endothelial Functionmentioning

confidence: 99%

The Levels of the Circulating Cellular Adhesion Molecules ICAM-1, VCAM-1 and Endothelin-1 and the Flow-mediated Vasodilatation Values in Patients with Type 1 Diabetes Mellitus with Early-stage Diabetic Retinopathy

et al. 2013

View full text Add to dashboard Cite

Objective Endothelial dysfunction plays an important role in the development of diabetic retinopathy. The aim of this study was to evaluate endothelial dysfunction using different approaches in patients with type 1 diabete mellitus with early stages of diabetic retinopathy. For this purpose, we investigated the serum levels of cellular adhesion molecules, including intercellular adhesion molecule (ICAM-1), vascular cell adhesion molecule (VCAM-1) and endothelin-1 (ET-1), which have emerged as specific markers of endothelial dysfunction, and measured the flow-mediated dilatation (FMD), a noninvasive technique used to evaluate endothelial dysfunction. Methods The study group included 59 patients with type 1 diabetes mellitus (DM) and 30 age-matched healthy control subjects. The diabetic patients were divided into two groups according to the ophthalmoscopic findings: Group 1, composed of type 1 diabetic patients having no signs of diabetic retinopathy (DRP), and Group 2, composed of type 1 diabetic patients having findings of the early stages of nonproliferative diabetic retinopathy (NPDR). Results The serum levels of ET-1 (fmol/mL), ICAM-1 (ng/mL) and VCAM-1 (ng/mL) were 8.52±0.699 vs. 478.39±46.22 vs. 728.64±35.081 in the patients without retinopathy, 8.91±1.354 vs. 451.79±48.262 vs. 863.59±62.37 in the diabetic patients with NPDR and 10.73±1.04 vs. 608.15±74.92 vs. 872.95±57.63 in the control group. There were no significant differences in the serum levels of the three molecules between the groups. The FMD values were 6.51±0.46% in the diabetic patients without retinopathy, 6.66±0.29% in the diabetic patients with NPDR and 6.68±0.51% in the control group. No significant differences were found between the groups. Conclusion The early stages of diabetic retinopathy cannot be considered in the evaluation of systemic markers of endothelial dysfunction.

show abstract

“…VCAM-1, a member of the immunoglobulin supergene family of cellular adhesion molecules, is involved in the recruitment of leukocytes, their adhesion to vascular endothelium, and their subsequent migration into surrounding tissue. Interestingly, the expression of VCAM-1 has been found in epiretinal membranes from diabetic patients with PDR 109,110 . In addition, it has been demonstrated that VCAM-1 promotes angiogenesis both in vitro and in vivo 111 …”

Section: Vascular Cell Adhesion Molecule (Vcam)mentioning

confidence: 99%

Inflammation and Angiogenesis in Diabetic Retinopathy

Yang¹,

Zhang²,

Li³

2012

Diabetic Retinopathy

View full text Add to dashboard Cite

Expression of intercellular adhesion molecule-1 (ICAM-1) and vascular cell adhesion molecule-1 (VCAM-1) on proliferating vascular endothelial cells in diabetic epiretinal membranes.

Abstract: The present study demonstrated the expression of intercellular adhesion molecule-i (ICAM-1) and vascular cell adhesion molecule-I (VCAM-1), the

Cited by 29 publications

References 31 publications

Vitreous Levels of Vascular Cell Adhesion Molecule and Vascular Endothelial Growth Factor in Patients With Proliferative Diabetic Retinopathy

Vitreous Levels of Vascular Cell Adhesion Molecule and Vascular Endothelial Growth Factor in Patients With Proliferative Diabetic Retinopathy

The Levels of the Circulating Cellular Adhesion Molecules ICAM-1, VCAM-1 and Endothelin-1 and the Flow-mediated Vasodilatation Values in Patients with Type 1 Diabetes Mellitus with Early-stage Diabetic Retinopathy

Inflammation and Angiogenesis in Diabetic Retinopathy

Contact Info

Product

Resources

About