Expression of key apoptotic genes in hepatocellular carcinoma cell line treated with etoposide-loaded graphene oxide

Gholami, Ahmad; Emadi, Fatemeh; Nazem, Maryam; Aghayi, Roghayeh; Khalvati, Bahman; Amini, Abbas; Ghasemi, Younes

doi:10.1016/j.jddst.2020.101725

Cited by 26 publications

(28 citation statements)

References 43 publications

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“…Our data confirm that carboxylation of nGO provides a lattice with better biocompatibility. In addition to reducing the cytotoxicity, carboxylation makes nGO a suitable carrier for loading many low-soluble drugs, especially anticancer agents [27,57,58]. As nGO-COOH is concentrated in intracellular vesicles, it can provide a promising platform for drug delivery.…”

Section: Journal Of Nanomaterialsmentioning

confidence: 99%

“…Researchers suggest that the functionalization of nGO with biocompatible molecules, such as polyethylene glycol (PEG), folic acid, and pluronic acid, can convert nGO to a nontoxic material [23][24][25][26]. Also, epoxide structures in the nGO family, when the ring is opened, were very reactive, which makes them more toxic; thus, converting the epoxide groups to the carboxyl group can lower the potential toxicity [27].…”

Section: Introductionmentioning

confidence: 99%

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Functionalization of Graphene Oxide Nanosheets Can Reduce Their Cytotoxicity to Dental Pulp Stem Cells

Gholami

Emadi

Amini

et al. 2020

Journal of Nanomaterials

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Background. The dental pulp is a heterogeneous soft tissue that supplies nutrients and acts as a biosensor to identify pathogenic stimuli. Regeneration of the dental pulp is one of the desirable topics for researchers. Graphene oxide nanosheets (nGOs) help overexpression of the genes related to odontogenic differentiation of stem cells from dental pulps and increases attachment and proliferation of dental pulp stem cells. Despite its benefits, nGO may be considered as a threat to the environment and human health. Therefore, the purpose of this study was to evaluate the biocompatibility potential of graphene oxide (nGO), chitosan functionalized graphene oxide (nGO-CS), and carboxylated graphene (nGO-COOH) when exposed to human dental pulp stem cells (hDPSCs). Material and Methods. Some different aspects of biocompatibility of nGO, nGO-CS, and nGO-COOH were synthesized, and several intracellular effects induced by different concentrations of graphene-based nanosheets, including cell viability, intracellular oxidative damages, and various factors such as LDH, GSH, SOD, MDA, and MMP, were studied on hDPSCs. Results. According to results, IC50 was determined as 232.01, 467.81, and ≥1000 μg/mL for nGO, nGO-CS, and nGO-COOH, respectively. These results demonstrated the lower toxicity and higher cytocompatibility of nGO-CS and nGO-COOH compared to nGO. nGO-COOH not only has any adverse effect on the cell membrane and mitochondrial activity but also shows slight antioxidant activity at some concentrations. Conclusion. The findings help design safe and cytocompatible nGO derivatives for biomedical applications in dental fields.

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Section: Journal Of Nanomaterialsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Functionalization of Graphene Oxide Nanosheets Can Reduce Their Cytotoxicity to Dental Pulp Stem Cells

Gholami

Emadi

Amini

et al. 2020

Journal of Nanomaterials

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“…[13][14][15][16] There are several studies showing that the anticancer effect of Etoposide is enhanced on different cancer cell lines using a nanoparticle delivery system. [17][18][19][20][21] Due to the hydrophobic nature of the drug PLGA and PCL were often used. For example, 11.8 micron PLGA nanoparticles loaded with Etoposide were evaluated for sustained pulmonary drug delivery.…”

Section: Introductionmentioning

confidence: 99%

“…There are several studies showing that the anticancer effect of Etoposide is enhanced on different cancer cell lines using a nanoparticle delivery system [17–21] . Due to the hydrophobic nature of the drug PLGA and PCL were often used.…”

Section: Introductionmentioning

confidence: 99%

Etoposide Loaded SPION‐PNIPAM Nanoparticles Improve the in vitro Therapeutic Outcome on Metastatic Prostate Cancer Cells via Enhanced Apoptosis

Erkısa

Arı

Ulku

et al. 2020

Chemistry & Biodiversity

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Prostate cancer is among the leading causes of death worldwide because its metastatic form is a deadly disease. Therefore, the development of new chemotherapeutics is of immense importance. Nanoparticle technology seems to provide diverse options in this regard. Therefore, poly(N-isopropylacrylamide) (PNIPAM) coated superparamagnetic iron oxide nanoparticles (SPION) loaded with Etoposide were prepared in small sizes (57 nm) and with 3.5 % drug content to improve the efficiency of Etoposide in prostate cancer therapy. Sustained release of the drug was achieved, which found to be sensitive to low pH and high temperature. The anti-growth activity of SPION-PNIPAM-Etoposide formulation against metastatic prostate cancer cells (PC-3, LNCaP) were investigated by SRB assay, then, confirmed by ATP assay. Mode of cell death was evaluated by using flow cytometry analyses. A significant improvement of nanoformulated drug was observed at 5-10 μg/ml doses of the drug in both cell lines. More importantly, this formulation enhanced the cytotoxic effect of Etoposide on PC-3 cells, which is considered more resistant to Etoposide than LNCaP and reduced the IC 50 value by 55 % reaching to 4.5 μg drug/ml, which is a very significant improvement in the literature. It was clearly shown that nanoformulated drug provided about 3-fold increases in caspase-dependent early apoptotic cells in PC-3 cells. The novel formulation seems to successfully cause cell death of especially PC-3 metastatic prostate cancer cells. It should therefore be taken into consideration for further animal studies as a novel potent anticancer agent.

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“…Among them, dispersive solid-phase extraction (dSPE) with the advantages of higher extraction recovery (ER), matrix simplification, eco-friendly, costeffective, and lower organic solvent volume is considered as a favorable, promising, and green technology [24,25]. In this regard, designing a proper sorbent to reach maximum efficiency is of high current interest [26][27][28]. A variety of porous sorbent materials have been developed including nanomaterials [29][30][31], zeolites [32], carbon nanotube [33,34], porous organic polymers [35,36], and metalorganic materials (MOMs) [37,38].…”

Section: Introductionmentioning

confidence: 99%

Rapid ultrasound‐assisted microextraction of atorvastatin in the sample of blood plasma by nickel metal organic modified with alumina nanoparticles

Bahrani

Asfaram

Mansoorkhani

et al. 2020

J of Separation Science

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In the present work, nickel-1,4-benzenedioxyacetic acid was synthesized as a rod-like metal organic material and then modified with alumina nanoparticles to synthesize nickel metal organic modified-Al 2 O 3 nanoparticles. The material was found as an efficient sorbent for the enrichment of atorvastatin in human blood plasma. After the extraction of the sample of plasma by ultrasound-assisted dispersive solid phase extraction, high performance liquid chromatographyultraviolet was used to determine the quantitatively pre-concentrated interest analyte. The conditions for optimum extraction were achieved by the optimization of the volume of eluent, dosage of the sorbent, and time of sonication. Solution pH of 7.0, 250 μL of ethanol, 45 mg of the sorbent, and 10 min of sonication time were the conditions for extracting the atorvastatin maximum recovery of higher than 97.0%. By using desirability function for the optimization of the process, the present method showed a response that was linear ranging from 0.2 to 800 ng/mL with regression coefficient of 0.999 in the plasma of human blood with a satisfactory detection limit of 0.05 ng/mL, while the precision of interday for the current method was found to be <5%. It can be concluded that dispersive solid phase extraction method is effective for the extraction of atorvastatin from human plasma samples (97.4-102%) due to its easy operation, simplicity, repeatability, and reliability.

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Expression of key apoptotic genes in hepatocellular carcinoma cell line treated with etoposide-loaded graphene oxide

Cited by 26 publications

References 43 publications

Functionalization of Graphene Oxide Nanosheets Can Reduce Their Cytotoxicity to Dental Pulp Stem Cells

Functionalization of Graphene Oxide Nanosheets Can Reduce Their Cytotoxicity to Dental Pulp Stem Cells

Etoposide Loaded SPION‐PNIPAM Nanoparticles Improve the in vitro Therapeutic Outcome on Metastatic Prostate Cancer Cells via Enhanced Apoptosis

Rapid ultrasound‐assisted microextraction of atorvastatin in the sample of blood plasma by nickel metal organic modified with alumina nanoparticles

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