2013
DOI: 10.3168/jds.2013-6849
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Expression of key lipid metabolism genes in adipose tissue is not altered by once-daily milking during a feed restriction of grazing dairy cows

Abstract: The objective of this study was to investigate the effect of reduced milking frequency, at 2 feeding levels, on gene expression in adipose tissue of grazing dairy cows during early lactation. Multiparous Holstein-Friesian and Holstein-Friesian × Jersey cows (n=120) were grazed on pasture and milked twice daily (2×) from calving to 34±6d in milk (mean ± standard deviation). Cows were then allocated to 1 of 4 treatments in a 2×2 factorial arrangement. Treatments consisted of 2 milking frequencies (2× or once dai… Show more

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Cited by 5 publications
(3 citation statements)
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“…Adipose Biopsy. Tissue was harvested during wk −1, 1, and 4 relative to parturition as described previ-ously (Grala et al, 2013). Briefly, subcutaneous adipose tissue was collected posterior to the shoulder blade and approximately 10 cm down the withers.…”
Section: Rna Extraction and Quantitative Pcrmentioning
confidence: 99%
“…Adipose Biopsy. Tissue was harvested during wk −1, 1, and 4 relative to parturition as described previ-ously (Grala et al, 2013). Briefly, subcutaneous adipose tissue was collected posterior to the shoulder blade and approximately 10 cm down the withers.…”
Section: Rna Extraction and Quantitative Pcrmentioning
confidence: 99%
“…Adipose Biopsies. Tissue was harvested during wk −1, 1, and 2 relative to calving as described previously (Grala et al, 2013). Briefly, subcutaneous adipose tissue was collected posterior to the shoulder blade and approximately 10 cm down the withers.…”
Section: Methodsmentioning
confidence: 99%
“…3168/ jds.2016-11790). Briefly, subcutaneous adipose tissue was collected posterior to the shoulder blade and approximately 10 cm down the withers during wk −1, 1, and 4 relative to parturition as described previously (Grala et al, 2013). Average sampling date (mean ± SD) for wk −1, 1, and 4 was −10.4 ± 2.4, 6.4 ± 0.9, and 27.4 ± 0.9 d relative to parturition, respectively.…”
Section: Rna Extraction and Quantitative Pcrmentioning
confidence: 99%