2005
DOI: 10.1016/j.abb.2004.10.026
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Expression of manganese lipoxygenase in Pichia pastoris and site-directed mutagenesis of putative metal ligands

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Cited by 46 publications
(72 citation statements)
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“…S1). 13R-MnLOX (602 amino acids) was previously expressed and secreted by P. pastoris using pPICZ␣_ MnLOX_602 (11). This plasmid was used to obtain shorter expression constructs.…”
Section: Methodsmentioning
confidence: 99%
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“…S1). 13R-MnLOX (602 amino acids) was previously expressed and secreted by P. pastoris using pPICZ␣_ MnLOX_602 (11). This plasmid was used to obtain shorter expression constructs.…”
Section: Methodsmentioning
confidence: 99%
“…First, the metal-coordinating spheres are not identical. The manganese ligands are likely three His residues, the carboxylate of the C-terminal amino acid residue, and an Asn residue in analogy to iron ligands of FeLOX (11,29), but the hexamer motif of FeLOX is truncated to a pentamer, HisVal-Leu-Phe-His, in MnLOX (11). Second, MnLOX oxidizes hydroperoxides to peroxyl radicals at a rate almost 2 orders of magnitude higher than that of sLOX-1 (30).…”
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confidence: 99%
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