Expression of Meiothermus ruber luxS in E. coli alters the antibiotic susceptibility and biofilm formation

Kaur, Amandeep; Capalash, Neena; Sharma, Prince

doi:10.1007/s00253-020-10480-8

Cited by 11 publications

(7 citation statements)

References 54 publications

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“…As shown in Figure 1 c, the accumulation concentration of AI-2 of L. plantarum SS-128 was at its highest in the late exponential phase and diminished slowly in the stationary phase. It was agreed with the results of Amandeep et al [ 29 ], which reported that AI-2 activity increased till the late exponential phase. Moreover, the results demonstrated that AI-2 activities of SS-128 were significantly increased at 14 h compared to those of Δ luxS /SS-128 combined with transcript-level expression analysis ( Figure 1 d).…”

Section: Resultssupporting

confidence: 93%

Dissecting of the AI-2/LuxS Mediated Growth Characteristics and Bacteriostatic Ability of Lactiplantibacillus plantarum SS-128 by Integration of Transcriptomics and Metabolomics

Qian

et al. 2022

Foods

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Lactiplantibacillus plantarum could regulate certain physiological functions through the AI-2/LuxS-mediated quorum sensing (QS) system. To explore the regulation mechanism on the growth characteristics and bacteriostatic ability of L. plantarum SS-128, a luxS mutant was constructed by a two-step homologous recombination. Compared with ΔluxS/SS-128, the metabolites of SS-128 had stronger bacteriostatic ability. The combined analysis of transcriptomics and metabolomics data showed that SS-128 exhibited higher pyruvate metabolic efficiency and energy input, followed by higher LDH level and metabolite overflow compared to ΔluxS/SS-128, resulting in stronger bacteriostatic ability. The absence of luxS induces a regulatory pathway that burdens the cysteine cycle by quantitatively drawing off central metabolic intermediaries. To accommodate this mutations, ΔluxS/SS-128 exhibited lower metabolite overflow and abnormal proliferation. These results demonstrate that the growth characteristic and metabolism of L. plantarum SS-128 are mediated by the AI-2/LuxS QS system, which is a positive regulator involved in food safety. It would be helpful to investigate more bio-preservation control potential of L. plantarum, especially when applied in food industrial biotechnology.

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Section: Resultssupporting

confidence: 93%

Dissecting of the AI-2/LuxS Mediated Growth Characteristics and Bacteriostatic Ability of Lactiplantibacillus plantarum SS-128 by Integration of Transcriptomics and Metabolomics

Qian

et al. 2022

Foods

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“…Notably, the accumulated concentration of AI-2 peaked in the late exponential phase and was slowly decreased during the stationary phase. These results are supported by the data of Kaur et al (2020) , who reported that the accumulation of AI-2 continues until the late exponential phase. The output of AI-2 was maximal at a high reproduction rate; hence, LAB cultured for 12 h were prepared for subsequent experiments.…”

Section: Resultssupporting

confidence: 87%

Amelioration of dextran sulfate sodium-induced colitis by autoinducer-2-deficient Lactiplantibacillus plantarum is mediated by anti-inflammatory effects and alleviation of dysbiosis of the gut microbiota

Qian

Zeng

et al. 2022

Front. Microbiol.

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Lactic acid bacteria (LAB) attenuate dextran sulfate sodium (DSS)-induced colitis in mice by restoring gut flora homeostasis and modulating the immune response. Because synchronous behavior can be controlled by autoinducer-2 (AI-2)/LuxS-mediated quorum sensing, the Caco-2 cell model and DSS-induced model in C57BL/6 mice were used to explore the unknown effects of these communications involving AI-2 among various intestinal symbiotic species. The results of the cell viability and lactate dehydrogenase leakage assays indicated that the tested strains (the wild-type strains and AI-2-deficient mutants) were characterized by equal cytoprotection from hydrogen peroxide-induced injury independently of AI-2. The results of the assays of multiple indicators and proinflammatory cytokines characteristic for the symptoms of colitis in mice showed that oral administration of AI-2-deficient mutants for 7 days was more effective in ameliorating inflammation than the treatment with the wild-type strains. The treatment with AI-2-deficient mutants enriched potential probiotics (e.g., Lactobacillaceae) and controlled the proliferation of potentially harmful bacteria (e.g., Helicobacteraceae) to achieve the transformation of intestinal flora. These mutants regulated short-chain fatty acids and the intestinal epithelial barrier, thereby promoting the maintenance of relatively favorable intestinal homeostasis. These results demonstrated that the AI-2-deficient mutants provided a more pronounced ameliorative effect on colitis in a mouse model, suggesting that the background of the LAB effect is associated with the alterations in colonic flora induced by AI-2.

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“…When cultured for 24 h, AI-2 activity was almost undetectable in the control group, while the AI-2 activity could still be detected in the treatment groups containing H 3 BO 3 and NaBO 2 . AI-2 activity increased till the late exponential phase and then diminished slowly in the stationary phase [ 23 ]. A possible reason was that the signal molecule AI-2 was re-absorbed by the bacteria or attached to their cell membrane, which regulated some essential physiological functions [ 24 ].…”

Section: Resultsmentioning

confidence: 99%

“…The recombinant E. coli was inoculated (1%, v/v ) in LB liquid medium containing 50 μg/mL ampicillin and cultured at 37 °C at 220 rpm. V. harveyi BB170 was inoculated (1%, v/v ) in marine broth 2216 medium The culture temperature was 30 °C, and the rotation speed was 220 rpm [ 23 ].…”

Section: Methodsmentioning

confidence: 99%

Boron Derivatives Accelerate Biofilm Formation of Recombinant Escherichia coli via Increasing Quorum Sensing System Autoinducer-2 Activity

Chen

Yan

Zhan

et al. 2022

IJMS

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Boron is an essential element for autoinducer-2 (AI-2) synthesis of quorum sensing (QS) system, which affects bacterial collective behavior. As a living biocatalyst, biofilms can stably catalyze the activity of intracellular enzymes. However, it is unclear how boron affects biofilm formation in E. coli, particularly recombinant E. coli with intracellular enzymes. This study screened different boron derivatives to explore their effect on biofilm formation. The stress response of biofilm formation to boron was illuminated by analyzing AI-2 activity, extracellular polymeric substances (EPS) composition, gene expression levels, etc. Results showed that boron derivatives promote AI-2 activity in QS system. After treatment with H3BO3 (0.6 mM), the AI-2 activity increased by 65.99%, while boron derivatives increased the biomass biofilms in the order H3BO3 > NaBO2 > Na2B4O7 > NaBO3. Moreover, treatment with H3BO3 (0.6 mM) increased biomass by 88.54%. Meanwhile, AI-2 activity had a linear correlation with polysaccharides and protein of EPS at 0–0.6 mM H3BO3 and NaBO2 (R2 > 0.8). Furthermore, H3BO3 upregulated the expression levels of biofilm formation genes, quorum sensing genes, and flagellar movement genes. These findings demonstrated that boron promoted biofilm formation by upregulating the expression levels of biofilm-related genes, improving the QS system AI-2 activity, and increasing EPS secretion in E. coli.

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Expression of Meiothermus ruber luxS in E. coli alters the antibiotic susceptibility and biofilm formation

Cited by 11 publications

References 54 publications

Dissecting of the AI-2/LuxS Mediated Growth Characteristics and Bacteriostatic Ability of Lactiplantibacillus plantarum SS-128 by Integration of Transcriptomics and Metabolomics

Dissecting of the AI-2/LuxS Mediated Growth Characteristics and Bacteriostatic Ability of Lactiplantibacillus plantarum SS-128 by Integration of Transcriptomics and Metabolomics

Amelioration of dextran sulfate sodium-induced colitis by autoinducer-2-deficient Lactiplantibacillus plantarum is mediated by anti-inflammatory effects and alleviation of dysbiosis of the gut microbiota

Boron Derivatives Accelerate Biofilm Formation of Recombinant Escherichia coli via Increasing Quorum Sensing System Autoinducer-2 Activity

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