Dissecting of the AI-2/LuxS Mediated Growth Characteristics and Bacteriostatic Ability of Lactiplantibacillus plantarum SS-128 by Integration of Transcriptomics and Metabolomics

Abstract

Lactiplantibacillus plantarum could regulate certain physiological functions through the AI-2/LuxS-mediated quorum sensing (QS) system. To explore the regulation mechanism on the growth characteristics and bacteriostatic ability of L. plantarum SS-128, a luxS mutant was constructed by a two-step homologous recombination. Compared with ΔluxS/SS-128, the metabolites of SS-128 had stronger bacteriostatic ability. The combined analysis of transcriptomics and metabolomics data showed that SS-128 exhibited higher pyruvate metabolic efficiency and energy input, followed by higher LDH level and metabolite overflow compared to ΔluxS/SS-128, resulting in stronger bacteriostatic ability. The absence of luxS induces a regulatory pathway that burdens the cysteine cycle by quantitatively drawing off central metabolic intermediaries. To accommodate this mutations, ΔluxS/SS-128 exhibited lower metabolite overflow and abnormal proliferation. These results demonstrate that the growth characteristic and metabolism of L. plantarum SS-128 are mediated by the AI-2/LuxS QS system, which is a positive regulator involved in food safety. It would be helpful to investigate more bio-preservation control potential of L. plantarum, especially when applied in food industrial biotechnology.

AI-2/Lux-S Quorum Sensing of Lactobacillus plantarum SS-128 Prolongs the Shelf Life of Shrimp (Litopenaeus vannamei): From Myofibril Simulation to Practical Application

“…In the control group, at the end of the storage, Shewanella occupied the highest composition of the bacteria (68.42%, 61.84%, 66.67%), followed by Carnobacterium (11.80%, 12.36%, 11.97%), and Vagococcus (13.29%, 15.14%, 12.86%). Shewanella has been reported to be one of the SSOs with high spoilage potential in Litopenaeus vannamei [ 43 ] and other aquatic products [ 6 , 44 ]. The abundance of Shewanella in the control group during storage indicated the spoilage of Litopenaeus vannamei .…”

Amelioration of dextran sulfate sodium-induced colitis by autoinducer-2-deficient Lactiplantibacillus plantarum is mediated by anti-inflammatory effects and alleviation of dysbiosis of the gut microbiota

“…Intracellular reactive oxygen species (ROS) were measured using the ROS Assay Kit (Beyotime Biotechnology, Shanghai, China). Cells were seeded in 48-well plates at the density of 2.0 × 10 4 cells per well for 48 h. Then 10 μM DCFH-DA (Beyotime Biotechnology, Shanghai, China) in serum-free culture medium cells were added into the cells for 20 min, and after washing with the Dulbecco’s PBS, the DCF (Beyotime Biotechnology, Shanghai, China)-loaded cells were incubated with or without 3-fold diluted cell-free filtrate in DMEM (CFF) of LAB and treated with 1 mM H 2 O 2 for 30 min ( Qian et al, 2022 ). MRS broth was used as blank control, and exogenous 4,5-Hydroxybiphenyl-2,3-pentanedione (DPD) concentration was 80 μM, which could spontaneously transform into AI-2 ( Winzer et al, 2003 ).…”

“…Lactiplantibacillus plantarum AB-1 and L. plantarum SS-128 are the two probiotics isolated from traditional dairy products and the fish intestine, respectively, and have been shown to produce AI-2 and exert beneficial effects, such as the production of antimicrobials ( Li Q. et al, 2019 ; Qian et al, 2022 ). The present study investigated the ameliorative effect of the two wild-types and corresponding luxS mutant strains (AI-2-defective mutants) on mice with DSS-induced colitis to assess a more efficient treatment for human IBD.…”

Bacteriocin production enhancing mechanism of Lactiplantibacillus paraplantarum RX-8 response to Wickerhamomyces anomalus Y-5 by transcriptomic and proteomic analyses

“…To identify the DEGs and DEPs of L. paraplantarum RX-8 in response to W. anomalus Y-5 under co-culture, the cells of L. paraplantarum RX-8 in co-culture and mono-culture were harvested at 24 h for transcriptomic and proteomic analyses. Due to a large amount of transcriptomic data and to reduce the false-positive rate, candidate genes of L. paraplantarum RX-8 involved in co-culture were chosen according to the following criteria: (1) more than 2-fold change after normalization; (2) Benjamini and Hochberg's correction approach was used to adjust the p-values; and (3) statistically significant level of p < 0.05. Finally, the transcription of 580 genes was detected, including 199 upregulated genes and 381 downregulated genes (Figure 3).…”