Expression of Messenger RNA for Prostaglandin E Receptor Subtypes EP4/EP2 and Cyclooxygenase Isozymes in Mouse Periovulatory Follicles and Oviducts During Superovulation1

Segi, Eri; Haraguchi, Kayo; Sugimoto, Yukihiko; Tsuji, Masazumi; Tsunekawa, Hiroko; Tamba, Shigero; Tsuboi, Kazuhito; Tanaka, Satoshi; Ichikawa, Atsushi

doi:10.1095/biolreprod.102.003590

Cited by 73 publications

(58 citation statements)

References 31 publications

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“…By contrast, higher PTGS2 mRNA levels occur in the cumulus cells of human oocytes expressing the highest developmental capacity (Gebhardt et al 2011). In cat COCs, we indeed observed increased PTGS2 mRNA expression after oocyte maturation in situ, which was consistent with earlier observations in the pig (Kawashima et al 2008) and mouse (Segi et al 2003). Nonetheless, the level of induction of PTGS2 transcription (post-eCG/hCG in intact cats) did not vary between seasons despite being clear compromises in oocyte developmental potential during both simulated and natural reproductively quiescent (compared to active) periods.…”

Section: Discussionsupporting

confidence: 91%

Absence of seasonal changes in FSHR gene expression in the cat cumulus–oocyte complex in vivo and in vitro

Hobbs

Howard²,

Wildt³

et al. 2012

Reproduction

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Domestic cat oocytes are seasonally sensitive to FSH. Compared with those collected during the breeding season, oocytes from the nonbreeding (NB) season require more FSH during in vitro maturation to achieve comparable developmental competence. This study tested the hypothesis that this seasonal variation was due to altered expression of FSH receptors (FSHR) and/or FSH-induced genes. Relative expression levels of FSHR mRNA and FSH-enhanced gene estrogen receptor b (ESR2) were measured by qPCR in whole ovaries and immature cumulus-oocyte complexes (COCs) isolated from cat ovaries during the natural breeding vs NB seasons. Expression levels of FSH-induced genes prostaglandin-endoperoxide synthase 2 (PTGS2), early growth response protein-1 (EGR1), and epidermal growth factor receptor (EGFR) were examined in mature COCs from both seasons that were a) recovered in vivo or b) matured in vitro with conventional (1 mg/ml) or high (10 mg/ml) FSH concentrations. Overall, FSHR mRNA levels were lower in whole ovaries during the NB compared with breeding season but were similar in immature COCs, whereas ESR2 levels did not differ in either group between intervals. We observed changes in PTGS2, EGR1, and EGFR mRNA expression patterns across maturation in COCs within but not between the two seasons. The lack of seasonal differentiation in FSH-related genes was not consistent with the decreased developmental capacity of oocytes fertilized during the NB season. These findings reveal that the seasonal decrease in cat oocyte sensitivity to FSH occurs both in vivo and in vitro. Furthermore, this decline is unrelated to changes in expression of FSHR mRNA or mRNA of FSH-induced genes in COCs from antral follicles.

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Section: Discussionsupporting

confidence: 91%

Absence of seasonal changes in FSHR gene expression in the cat cumulus–oocyte complex in vivo and in vitro

Hobbs

Howard²,

Wildt³

et al. 2012

Reproduction

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“…Second, these processes are attenuated by a mixed EP2/EP4 antagonist. Third, EP2, EP3, and EP4 are expressed in cumulus cells, and hCG up-regulates EP2 and EP4 expression in these cells (28). However, the role of EP3 in cumulus cell function does not appear to be critical, because EP3 null females do not exhibit overt reproductive phenotypes (11).…”

Section: Discussionmentioning

confidence: 99%

“…Vitro-Previous studies have demonstrated a spatiotemporal expression of COX-2 in mural granulosa and cumulus cells in response to preovulatory gonadotropin stimulation in mice (24,27,28). To further elucidate the physiological significance of COX-2-derived PGs in oocyte maturation in vitro, we first assessed COX-2 expression by immunofluorescence in wild-type COCs in culture.…”

Section: Pge 2 Differentially Regulates Oocyte Maturation and Cumulusmentioning

confidence: 99%

Cyclooxygenase-2-derived Prostaglandin E2 Directs Oocyte Maturation by Differentially Influencing Multiple Signaling Pathways

Takahashi

Morrow

Wang

et al. 2006

Journal of Biological Chemistry

130

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The process of oocyte maturation, which impacts ovulation and fertilization, is complex and requires an integration of the endocrine, paracrine, juxtacrine, and autocrine signaling pathways. This process involves an intimate interaction between the oocyte and encircling cumulus cells within a follicle, a unique venue for somatic and germ cell communication. Cumulus cell expansion and resumption of meiosis with germinal vesicle breakdown are major events in oocyte maturation. Cyclooxygenase-2 (COX-2)-derived prostaglandin E 2 (PGE 2 ) is a known critical mediator of oocyte maturation, but the diverse function of this lipid mediator in oocyte maturation, ovulation, and fertilization has not been fully appreciated. We show here that gonadotropins in coordination with PGE 2 signaling via its cell surface G-protein-coupled EP2 and EP4 receptor subtypes direct cumulus cell expansion and survival and oocyte meiotic maturation by differentially impacting cAMPdependent protein kinase, MAPK, NF-B, and phosphatidylinositol 3-kinase/Akt pathways. This study is unique in the sense that it provides evidence for new site-and event-specific involvement of these signaling pathways under the influence of COX-2-derived PGE 2 during the critical stages of this somatic-germ cell interaction, an absolute requirement for oocyte maturation. Prostaglandins (PGs)4 are involved in various female reproductive functions, including ovulation, fertilization, and implantation (1, 2). The cyclooxygenase (COX)

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“…Ptgs2 is a crucial factor for ovulation and is generally induced around 3-h post-hCG (Lim et al 1997, Joyce et al 2001, Segi et al 2003. As shown in Fig.…”

Section: Resultsmentioning

confidence: 97%

Etv5, an ETS transcription factor, is expressed in granulosa and cumulus cells and serves as a transcriptional regulator of the cyclooxygenase-2

Eo¹,

Han²,

Murphy³

et al. 2008

Journal of Endocrinology

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Etv4, Etv1, and Etv5 are members of Etv4 subfamily of E26 transformation-specific (Ets) transcription factors that are known to influence a host of biological processes. We previously showed that Etv5, expressed in Sertoli cells, plays a crucial role in maintaining spermatogonial stem cell niche in the mouse testis. However, it is not yet known whether Etv4 family members are expressed in the ovary or play any role in ovarian functions. Here, we show that Etv5 and Etv4 are expressed in mouse ovaries in granulosa and cumulus cells during folliculogenesis. Both Etv5 and Etv4 mRNAs are also detected in cumulusoocyte complexes (COCs) and denuded oocytes. Notably, Etv4 is highly expressed in the cumulus cells of ovulated COCs at 16-h post-human chorionic gonadotropin. Cyclooxygenase-2 (PTGS2), a rate-limiting enzyme for prostaglandin synthesis, is critical for oocyte maturation and ovulation. Since several putative Ets-binding sites are present in the PTGS2 promoter, we examined whether Etv5 influences Ptgs2 transcriptional activity. Indeed, we found that addition of Etv5 increases the transcriptional activity of the 3 . 2-kb mouse Ptgs2 promoter by 2 . 5-fold in luciferase reporter assays. Collectively, the results show that Etv4 and Etv5 are expressed in granulosa and cumulus cells during folliculogenesis and ovulation, suggesting that they influence cellular events in the ovary by regulating downstream genes such as Ptgs2.

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Expression of Messenger RNA for Prostaglandin E Receptor Subtypes EP4/EP2 and Cyclooxygenase Isozymes in Mouse Periovulatory Follicles and Oviducts During Superovulation1

Cited by 73 publications

References 31 publications

Absence of seasonal changes in FSHR gene expression in the cat cumulus–oocyte complex in vivo and in vitro

Absence of seasonal changes in FSHR gene expression in the cat cumulus–oocyte complex in vivo and in vitro

Cyclooxygenase-2-derived Prostaglandin E2 Directs Oocyte Maturation by Differentially Influencing Multiple Signaling Pathways

Etv5, an ETS transcription factor, is expressed in granulosa and cumulus cells and serves as a transcriptional regulator of the cyclooxygenase-2

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