Expression of MHC-I mRNA in Peripheral Blood Lymphocytes as an Early Marker of Acute Rejection Following Skin Transplantation in Mice

Wan, Furong; Lü, Nan; Zou, Xiong; Zhang, Yi; Shan, Ning-ning; Xj, Yang; Li, Xiangdong; Zhao, Sixiang; Wang, Shenghua; Zhu, Min; Li, Xiaoli; Li, Yingjie; Zhou, Yüxia; Li, Hao

doi:10.1620/tjem.215.79

Cited by 5 publications

(3 citation statements)

References 12 publications

(10 reference statements)

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“…Immune rejection is still a key factor to consider when transplanting tissue-engineered skin. Expression of MHC-I can be used as an important immune rejection marker during the early period of tissue-engineered skin transplantation [ 49 , 50 ]. In our study, there were a few red dots within the first 14 days in Groups A and B (Figure 10 a,b,d,e).…”

Section: Discussionmentioning

confidence: 99%

Gelatin-chondroitin-6-sulfate-hyaluronic acid scaffold seeded with vascular endothelial growth factor 165 modified hair follicle stem cells as a three-dimensional skin substitute

Quan

Zheng

et al. 2014

Stem Cell Res Ther

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IntroductionIn the field of skin tissue engineering, gelatin-chondroitin-6-sulfate-hyaluronic acid (Gel-C6S-HA) stents are a suitable bio skin substitute. The purpose was to investigate the effect of genetically-modified hair follicle stem cells (HFSCs), combined with Gel-C6S-HA scaffolds, on the vascularization of tissue-engineered skin.MethodsThree-dimensional (3D) Gel-C6S-HA scaffolds were prepared by freeze-drying. Vascular endothelial growth factor (VEGF) 165 gene-modified rat HFSCs (rHFSCs) were inoculated into the scaffolds and cultured for 7 days. Two bilateral full-thickness skin defects were created on the back of 18 Sprague–Dawley rats. Rats were randomly divided into four groups: Group A, HFSCs transduced with VEGF165 seeded onto Gel-C6S-HA scaffolds; Group B, HFSCs transduced with empty vector seeded onto Gel-C6S-HA scaffolds; Group C, Gel-C6S-HA scaffold only; Group D, Vaseline gauze dressing. These compositions were implanted onto the defects and harvested at 7, 14 and 21 days. Wound healing was assessed and compared among groups according to hematoxylin-eosin staining, CD31 expression, alpha smooth muscle actin (α-SMA) and major histocompatibility complex class I (MHC-I) immunohistochemistry, and microvessel density (MVD) count, to evaluate the new blood vessels.ResultsSEM revealed the Gel-C6S-HA scaffold was spongy and 3D, with an average pore diameter of 133.23 ± 43.36 μm. Cells seeded on scaffolds showed good adherent growth after 7 days culture. No significant difference in rHFSC morphology, adherence and proliferative capacity was found before and after transfection (P >0.05). After 14 and 21 days, the highest rate of wound healing was observed in Group A (P <0.05). Histological and immunological examination showed that after 21 days, MVD also reached a maximum in Group A (P <0.05). Therefore, the number of new blood vessels formed within the skin substitutes was greatest in Group A, followed by Group B. In Group C, only trace amounts of mature subcutaneous blood vessels were observed, and few subcutaneous tissue cells migrated into the scaffolds.ConclusionsTissue-engineered skin constructs, using 3D Gel-C6S-HA scaffolds seeded with VEGF165-modified rHFSCs, resulted in promotion of angiogenesis during wound healing and facilitation of vascularization in skin substitutes. This may be a novel approach for tissue-engineered skin substitutes.

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Section: Discussionmentioning

confidence: 99%

Gelatin-chondroitin-6-sulfate-hyaluronic acid scaffold seeded with vascular endothelial growth factor 165 modified hair follicle stem cells as a three-dimensional skin substitute

Quan

Zheng

et al. 2014

Stem Cell Res Ther

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“…This is similar to the trend in the expression of MHC-I mRNA seen previously. 18,24 In addition, increased levels of HLA-I antigens on peripheral blood lymphocytes have been reported in liver transplant rejection 25 and cardiac transplant rejection. 26 This suggests that MHC-I antigen upregulation on peripheral CD3 + CD8 + T-cells is a universal characteristic of organ transplantation rejection.…”

Section: Discussionmentioning

confidence: 99%

“…16,17 In a previous study, our research group showed that increased expression of MHC-I mRNA in peripheral blood lymphocytes was correlated with AGR in mice, suggesting that MHC-I mRNA may be an indicator of AGR. 18 However, because MHC-I mRNA is unstable and easily degradable and its measurement is complicated and time-consuming, it is unsuitable as a clinical marker for AGR. In addition, the expressions of MHC mRNA and MHC protein have been reported to have no direct correlation.…”

Section: Mhc-i Expression On T-lymphocytes In Acute Graft Rejectionmentioning

confidence: 99%

Acute Rejection Correlates with Expression of Major Histocompatibility Complex Class I Antigens on Peripheral Blood CD3⁺CD8⁺ T-Lymphocytes following Skin Transplantation in Mice

et al. 2011

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This study investigated major histocompatibility complex class I (MHC-I) antigen expression on peripheral blood T-cells after transplantation to assess its potential as an early marker of acute graft rejection (AGR). Using a mouse model with or without immunosuppressive treatment, the expression of MHC-I antigens on CD3(+)CD8(+) T-lymphocytes was assessed by flow cytometry following syngeneic graft (n = 138) or allograft (n = 138) skin transplantation. The occurrence of AGR was assessed by examining the degree of lymphocyte and monocyte infiltration in transplant biopsies. During AGR, expression of MHC-I antigens increased significantly compared with pre-transplant levels in the allograft group, even with immunosuppressive treatment. The highest expression of MHC-I antigens occurred 5 - 6 days before macroscopic rejection. These results suggest that expression of MHC-I antigens on peripheral blood CD3(+)CD8(+) T-lymphocytes could be used as an early marker for predicting AGR.

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Peripheral blood transcriptome sequencing reveals rejection-relevant genes in long-term heart transplantation

Chen

Zhang

Xiao

et al. 2013

International Journal of Cardiology

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Expression of MHC-I mRNA in Peripheral Blood Lymphocytes as an Early Marker of Acute Rejection Following Skin Transplantation in Mice

Cited by 5 publications

References 12 publications

Gelatin-chondroitin-6-sulfate-hyaluronic acid scaffold seeded with vascular endothelial growth factor 165 modified hair follicle stem cells as a three-dimensional skin substitute

Gelatin-chondroitin-6-sulfate-hyaluronic acid scaffold seeded with vascular endothelial growth factor 165 modified hair follicle stem cells as a three-dimensional skin substitute

Acute Rejection Correlates with Expression of Major Histocompatibility Complex Class I Antigens on Peripheral Blood CD3⁺CD8⁺ T-Lymphocytes following Skin Transplantation in Mice

Peripheral blood transcriptome sequencing reveals rejection-relevant genes in long-term heart transplantation

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Expression of MHC-I mRNA in Peripheral Blood Lymphocytes as an Early Marker of Acute Rejection Following Skin Transplantation in Mice

Cited by 5 publications

References 12 publications

Gelatin-chondroitin-6-sulfate-hyaluronic acid scaffold seeded with vascular endothelial growth factor 165 modified hair follicle stem cells as a three-dimensional skin substitute

Gelatin-chondroitin-6-sulfate-hyaluronic acid scaffold seeded with vascular endothelial growth factor 165 modified hair follicle stem cells as a three-dimensional skin substitute

Acute Rejection Correlates with Expression of Major Histocompatibility Complex Class I Antigens on Peripheral Blood CD3+CD8+ T-Lymphocytes following Skin Transplantation in Mice

Peripheral blood transcriptome sequencing reveals rejection-relevant genes in long-term heart transplantation

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Acute Rejection Correlates with Expression of Major Histocompatibility Complex Class I Antigens on Peripheral Blood CD3⁺CD8⁺ T-Lymphocytes following Skin Transplantation in Mice