Expression of miRNA-21, miRNA-107, miRNA-137 and miRNA-29b in meningioma

Katar, Salim; Baran, Oğuz; Evran, Şevket; Çevik, Serdar; Akkaya, Enes; Baran, Gözde; Antar, Veysel; Hanımoğlu, Hakan; Kaynar, Mehmet Yaşar

doi:10.1016/j.clineuro.2017.03.016

Cited by 25 publications

(25 citation statements)

References 25 publications

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“… 24 Another study revealed that high-expressed miR-21 expression and low-expressed miR-107 were closely associated with histopathological grade of meningiomas. 25 miRNA-145 was found to be downregulated in meningioma, and elevated miR-145 expression in IOMM-Lee meningioma cells led to reduced proliferation, induced apoptosis and impaired migratory and invasive potential. 15 Moreover, this study also revealed a significant downregulation of let-7d expression in meningioma tumor tissues.…”

Section: Discussionmentioning

confidence: 93%

<em>let-7d</em> suppresses proliferation and invasion and promotes apoptosis of meningioma by targeting AEG-1

Zhao

2017

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Backgroundlet-7d has been indicated to act as a tumor suppressor in various cancers. However, the function and molecular mechanism of let-7d in meningioma progression have not been elucidated.Materials and methodsQuantitative real-time polymerase chain reaction (qRT-PCR) was performed to detect the expression levels of let-7d and AEG-1 mRNA in meningioma tissues and cell lines. The protein level of AEG-1 was measured by Western blot analysis. MTT assay, Transwell invasion assay and flow cytometry analysis were carried out to determine the proliferation, invasion and apoptosis of IOMM-Lee and CH-157MN cells, respectively. Target gene of let-7d was verified by luciferase reporter analysis.Resultslet-7d expression was downregulated, and AEG-1 expression was upregulated in meningioma tumor tissues. let-7d overexpression suppressed proliferation and invasion and induced apoptosis in IOMM-Lee and CH-157MN cells. Moreover, AEG-1 was a direct target of let-7d. Restoration of AEG-1 expression reversed let-7d-mediated suppression of the proliferation and invasion and let-7d-induced apoptosis in IOMM-Lee and CH-157MN cells.Conclusionlet-7d repressed proliferation and invasion and promoted apoptosis of meningioma cells by targeting AEG-1. The present study provided a better understanding of the meningioma pathogenesis and a promising therapeutic target for meningioma patients.

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Section: Discussionmentioning

confidence: 93%

<em>let-7d</em> suppresses proliferation and invasion and promotes apoptosis of meningioma by targeting AEG-1

Zhao

2017

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“…MiRNA expression profiling revealed high and low expression of a variety of miRNAs which are linked to higher recurrence rates of meningiomas [64]. Downregulation of miR-200a plays a pivotal role in the development of benign meningiomas through the upregulation of three mRNA targets while miR-21 and 34a have represented barriers for grades 1 and 2 tumors to malignant transition [65,66]. MiRNA expression in meningiomas is varied across grades and especially plays a key role in the early stages of tumorigenesis and may provide a potential therapeutic target in the future.…”

Section: Epigenetic and Mrna Markersmentioning

confidence: 99%

Advances in meningioma genomics, proteomics, and epigenetics: insights into biomarker identification and targeted therapies

2020

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“…Genome-wide studies have demonstrated that miRNA genes are frequently located in cancer-associated genomic regions, indicating the potential roles of miRNAs in tumorigenesis (17). Previous studies on meningioma have suggested that several miRNAs participate in the regulation of cell proliferation (18)(19)(20)(21), apoptosis (19,22), invasiveness (19,23), migration (19,24), tumor recurrence (25)(26)(27), and histopathological progression (18,19,25,(27)(28)(29). However, no miRNAs have been verified to affect the radiosensitivity of meningiomas.…”

Section: Introductionmentioning

confidence: 99%

MicroRNA-221/222 Inhibits the Radiation-Induced Invasiveness and Promotes the Radiosensitivity of Malignant Meningioma Cells

et al. 2020

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The controversy of adjuvant radiotherapy of meningiomas is at least partially due to the insufficient understanding on meningioma cells' response to irradiation and the shortage of radiosensitivity-promotion methods. MicroRNA-221 and microRNA-222 were identified as critical regulators of radiosensitivity in several other tumors. However, their effect in meningiomas has yet to be confirmed. Therefore, the malignant meningioma IOMM-Lee cells were adopted, transfected with microRNA-221/222 mimics or inhibitors, and irradiated with different dosages. The effects of radiation and microRNA-221/222 were then assessed in vitro and in vivo . Radiation dose increases and microRNA-221/222 downregulation synergistically inhibited cell proliferation and colony formation, prevented xenograft tumor progression, and promoted apoptosis, but antagonistically regulated cell invasiveness. Pairwise comparisons revealed that only high-dose radiations (6 and 8 Gy) can significantly promote cell invasiveness in comparison with unirradiated counterparts. Further comparisons exhibited that downregulating the microRNA-221/222 expression can reverse this radiation-induced cell invasiveness to a level of untransfected and unirradiated cells only if cells were irradiated with no more than 6 Gy. In addition, this approach can promote IOMM-Lee's radiosensitivity. Meanwhile, we also detected that the dose rate of irradiation affects cell cycle distribution and cell apoptosis of IOMM-Lee. A high dose rate irradiation induces G0/G1 cell cycle arrest and apoptosis-promoting effect. Therefore, for malignant meningiomas, high-dose irradiation can facilitate cell invasiveness significantly. Downregulating the microRNA-221/222 level can reverse the radiation-induced cell invasiveness while enhancing the apoptosis-promoting and proliferation-inhibiting effects of radiation and promoting cell radiosensitivity.

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Expression of miRNA-21, miRNA-107, miRNA-137 and miRNA-29b in meningioma

Cited by 25 publications

References 25 publications

<em>let-7d</em> suppresses proliferation and invasion and promotes apoptosis of meningioma by targeting AEG-1

<em>let-7d</em> suppresses proliferation and invasion and promotes apoptosis of meningioma by targeting AEG-1

Advances in meningioma genomics, proteomics, and epigenetics: insights into biomarker identification and targeted therapies

MicroRNA-221/222 Inhibits the Radiation-Induced Invasiveness and Promotes the Radiosensitivity of Malignant Meningioma Cells

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