Expression of miRNAs in the colon, rectum, plasma, and feces in rats with dextran sulfate sodium

Fecal microRNA223 as an indicator of recovery in chronic DSS colitis model in rats

“…At the end of the off-dose period of Cycle 2, the histological changes in the rectum worsened, while the colon changes showed recovery. The expression patterns of all miRNAs were almost the same in Cycle 2 when compared to those in a previous study (Kodama et al, 2021). Fecal miR-223-3p could be also a useful non-invasive indicator to evaluate the reversibility in chronic DSS-induced colitis in rats.…”

“…1. The DSS dosage levels were selected based on the results of a previous study (Kodama et al, 2021). During the dosing and recovery periods, the rats were observed once daily for general clinical signs and stools.…”

“…In addition to clinical research, up-or downregulation of several miRNAs have been reported in patients with IBD. The administration of dextran sulfate sodium (DSS) to mice (Melgar et al, 2005) and rats (Kodama et al, 2021) resulted in toxicity in the large intestine, including inflammation and epithelial damage similar to IBD; thus, the DSS-induced colitis model is widely used as a nonclinical IBD model. Kodama et al (2021) showed that in the rat DSS-induced colitis model, miR-31a, miR-181b, and miR-223 levels in plasma and feces were significant-ly reduced after DSS treatment, and those in feces were increased after the recovery period.…”

“…The administration of dextran sulfate sodium (DSS) to mice (Melgar et al, 2005) and rats (Kodama et al, 2021) resulted in toxicity in the large intestine, including inflammation and epithelial damage similar to IBD; thus, the DSS-induced colitis model is widely used as a nonclinical IBD model. Kodama et al (2021) showed that in the rat DSS-induced colitis model, miR-31a, miR-181b, and miR-223 levels in plasma and feces were significant-ly reduced after DSS treatment, and those in feces were increased after the recovery period. The miRNA expression profiles dynamically changed either by overexpression (miR-181b, miR-31, miR-146b, miR-221, and miR-155) or by suppression (miR-34a) through NF-κB-STAT3 crosstalk in azoxymethane (AOM)/DSS-induced colon tumorigenesis in mice (El-Daly et al, 2019).…”

“…The miRNA expression profiles dynamically changed either by overexpression (miR-181b, miR-31, miR-146b, miR-221, and miR-155) or by suppression (miR-34a) through NF-κB-STAT3 crosstalk in azoxymethane (AOM)/DSS-induced colon tumorigenesis in mice (El-Daly et al, 2019). Fecal miR-31a-5p, miR-181b-5p, and miR-223-3p could be used as non-invasive biomarkers translatable in animals and humans (Kodama et al, 2021). Multiple cycles of repeated DSS administration resulted in a chronic IBD model (Eichele and Kharbanda, 2017).…”