Expression of MMP-8 and MMP-13 Genes in the Periodontal Ligament during                Tooth Movement in Rats

Takahashi, Ichiro; Nishimura, Makoto; Onodera, Kazuyuki; Bae, Jin Wan; Okazaki, Mitsuko; Sasano, Yasuyuki; Mitani, Hideo

doi:10.1177/154405910308200815

Cited by 89 publications

(68 citation statements)

References 25 publications

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“…The results of the present study are consistent with those of previous studies on the effects of age on PDL width 4 and on changes in PDL width during the early phase of force application in young animals 22 ; a timerelated decrease in the PDL SA is evident in adult rat molars, as is a decreased or increased SA at compression or tension regions, respectively, at week 1 in young rats. Such a region-related difference in adult rats, however, started a week later, and was not significant until week 8, when a considerable amount of tooth movement and osteoclastic bone resorption occurred, as was also shown in earlier studies.…”

Section: Discussionsupporting

confidence: 82%

Age-Related Changes of Periodontal Ligament Surface Areas during Force Application

Ren

Maltha

Stokroos

et al. 2008

The Angle Orthodontist

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Objective: To investigate the age-dependent morphology of the periodontal ligament (PDL) tissue and changes in its surface area (SA) during force application provided with a standardized orthodontic setup for a period of 12 weeks in young and adult rats. Methods: Two groups of 30 rats, age 6 weeks and 9 to 12 months, were used. Orthodontic appliances were placed to move the maxillary molars mesially with the contralateral sides used as controls. At 1, 2, 4, 8, and 12 weeks, groups of animals were killed. The PDL SA and the PDL SA ratio between pressure and tension regions were determined. Results: An age-related decrease in the PDL SA was noted at control sides. Significant changes during the experimental period occurred only at experimental sides: The PDL SA was smaller at pressure than at tension regions only at week 1 in young rats; in adult rats, the difference between the two regions was significant at week 8. These changes were confirmed by the morphologic disorganization of the PDL and alterations in the PDL SA ratio. Conclusions: During force application, the PDL at the pressure regions became disorganized and subsequently was reorganized, as is shown by the histologic changes and SA of the PDL over time. This process occurred earlier and was more prominent in young rats; it occurred later and was more prolonged in adult animals.

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Section: Discussionsupporting

confidence: 82%

Age-Related Changes of Periodontal Ligament Surface Areas during Force Application

Ren

Maltha

Stokroos

et al. 2008

The Angle Orthodontist

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“…These results are consistent with previous research, that there is increasing of MMP expression during orthodontic tooth movement on both the pressure and tension side [6,[19][20][21]. Maxwell, et al (2001) stated that the condition of diabetes is often associated with the presence of infla-mmation, the formation of ROS and increasing of MMP 9 [22].…”

Section: Expression Of Mmp 9 and Number Of Fibroblastssupporting

confidence: 91%

“…On the pressure side, the compression of periodontal ligament causes ligament fibers sagging, and fiber degradation appears to be increasing [37]. Periodontal ligament remodeling requires regulation of MMPs and TIMPs [21], and the expressions of TIMP 1 on the tension side were increased in osteoblasts during bone formation [38].…”

Section: Expression Of Mmp 9 and Number Of Fibroblastsmentioning

confidence: 99%

The Effect of Different Orthodontic Force on MMP 9 Expression in a Rat Diabetic Model

Maulana¹,

Hikmah²,

Shita³

et al. 2014

JTLS

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Orthodontic forces produce alveolar bone and periodontal ligament remodeling that cause tooth movement. Diabetes can increase tissue damage and, therefore, contributes to the process of orthodontic tooth movement. The aim of this study was to determine the effect of differently orthodontic forces on the expression of MMP nine in rat diabetic model. Twenty four male Wistar rats were divided into three groups of control and three groups of treatment strept ozotocin-induced diabetes with stratified doses. Application of orthodontic appliance was performed with different forces (10, 20 and 30 grF). Orthodontic appliance was performed on both upper incisors. Immunohistochemical (IHC) analysis was used to observe the expression of MMP 9 and HE (Hematoxylen Eosin) staining to observe the number of fibroblasts. Statistical analysis was carried out using t -test and Spearman correlation analysis to determine the difference of MMP nine expression and number of fibroblasts between groups, and determine the correlation of both. The results showed an increased expression of MMP 9 and decreased of fibroblasts number in diabetic rats, along with increasing magnitude of orthodontic forces. The different orthodontic forces given to diabetic rats affect the expression of MMP 9 and the number of fibroblasts, in which the expression of MMP 9 increased along with the increase of orthodontic forces, both at pressure and tension sides..

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“…15 Also, mRNA of MMP-13 levels has been detected both on the tension and compression sides in rodents, and MMP-13 gene expression seems to be time-dependent and differentially regulated. 13 Reporting on the effect of drugs administered during induced tooth movement is still lacking standardized methodology in many aspects. Results reported are controversial and inconsistent leading to misinterpretation.…”

mentioning

confidence: 99%

The use of different analgesics in orthodontic tooth movements

Hammad¹,

El-Hawary²,

El-Hawary³

2012

The Angle Orthodontist

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Objective: To provide a semi-quantitative assessment of the effect of different analgesics (celecoxib, ketorolac, and paracetamol) on tooth movement and bone resorption using immunohistochemical staining of matrix metalloproteinase-13 (MMP-13). Materials and Methods: Forty white male rats (12-weeks old; body weight: 230-250 g) were divided into four groups (10 rats each) and were given the treatment once a day for 2 consecutive months. Group A (control group) rats were given the reverse osmosis water; group B rats were given 10 mg/kg celecoxib; group C rats were given 3 mg/kg ketorolac; and group D rats were given 150 mg/kg paracetamol. A precalibrated closed Sentalloy coil spring was placed inside each rat mouth to deliver a constant force of 50 cN. The magnitude of tooth movement was measured intraorally. After 2 months, the rats were sacrificed, and the sections were mounted on Lpolylysine-coated glass slides. Slides from each specimen were stained with hematoxylin and eosin, and others were stained with MMP-13. Data were analyzed with the one-way analysis of variance (ANOVA). Results: Celecoxib, ketorolac, and paracetamol groups showed tooth movement of 1.81 6 0.43 mm, 1.13 6 0.28 mm, and 1.08 6 0.27 mm, respectively. The mean number of MMP-13-positive osteoclasts was highest in celecoxib-treated group followed by the control group and was decreased in the ketorolac and paracetamol groups. Comparing all groups to the control revealed significant differences (P , .05). Conclusion: Administration of celecoxib did not reduce bone resorption or interfere with tooth movement in rats compared to other analgesics tested (ketorolac and paracetamol). (Angle Orthod. 2012;82:820-826.)

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Expression of MMP-8 and MMP-13 Genes in the Periodontal Ligament during Tooth Movement in Rats

Cited by 89 publications

References 25 publications

Age-Related Changes of Periodontal Ligament Surface Areas during Force Application

Age-Related Changes of Periodontal Ligament Surface Areas during Force Application

The Effect of Different Orthodontic Force on MMP 9 Expression in a Rat Diabetic Model

The use of different analgesics in orthodontic tooth movements

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