Expression of Monocyte Chemotactic Protein (MCP)-1, MCP-2, and MCP-3 by Human Airway Smooth-Muscle Cells

Abstract

We have demonstrated that, in addition to their contractile function, human airway smooth-muscle cells (HASMC) are able to express and to secrete chemokines of the monocyte chemotactic protein (MCP)/ eotaxin subfamily. This group of chemokines is believed to play a fundamental role in the development of allergic airway diseases such as asthma. The expression levels of MCP (MCP-1, -2, and -3) messenger RNA (mRNA) were compared with those of regulated on activation, normal T cells expressed and secreted (RANTES) mRNA in HASMC in culture. HASMC express MCP and RANTES mRNA after stimulation with interleukin (IL)-1beta, tumor necrosis factor-alpha, and interferon-gamma. MCP mRNA was maximal at 8 h, whereas RANTES mRNA expression was delayed to 24 h after stimulation. Further, significant differences were observed in the induction patterns of MCP and RANTES mRNA expression after stimulation with the individual cytokines. Dexamethasone (DEX) significantly inhibited cytokine-induced accumulation of MCP and RANTES mRNA, in contrast to IL-4, IL-10, and IL-13, which had no inhibitory effect on cytokine-induced chemokine expression. The cytokine-induced MCP mRNA expression in HASMC was associated with MCP release, which was inhibited by DEX and post-translationally by IL-4. HASMC can actively participate in the pathogenesis of asthma by the expression and release of chemokines, which are likely to play a critical role in the generation and regulation of the inflammatory response characteristic of allergic airway diseases.

Monocyte Chemoattractant Protein-1 (CCL-2) Integrates Mechanical and Endocrine Signals That Mediate Term and Preterm Labor

“…Using an immunohistological approach we confirmed that Ccl-2 protein was expressed predominantly by rat myometrial SMCs. Because there is evidence that chemokines from the MCP subfamily of cytokines are likely to play a critical role in the regulation of the inflammatory response in other type of SMCs (40), our findings demonstrate that myometrial cells may contribute directly to the development of uterine inflammation by promoting the recruitment of monocytes to term myometrium, which is one of the hallmarks of parturition.…”

Identification of PDL-1 as a novel biomarker of sensitizer exposure in dendritic-like cells

“…Sakaguchi et al (2006) also observed variable responses depending on both the sensitizer and cell type used suggesting that sensitizers act differently depending on the cellular system (Sakaguchi et al, 2006). Recent work has indicated that the cell responsiveness to sensitizers is affected by external 'danger signals' (Lavergne et al, 2009) and hapten:protein interactions (Jenkinson et al, 2009) hours (Goldstein et al, 1996;Pype et al, 1999). Therefore, with regards to moDC MCP-1 secretion, optimisation of the time point for maximal detection may be required.…”

Role of Monocyte Chemotactic Protein-1/CC Chemokine Ligand 2 on γδ T Lymphocyte Trafficking during Inflammation Induced by Lipopolysaccharide orMycobacterium bovisBacille Calmette-Guérin

“…Smith and Herschman (20) identified cDNAs for seven LPS-induced genes for chemokines that were attenuated by glucocorticoid, including MCP-1. Several authors have demonstrated that dexamethasone inhibits MCP-1 gene expression by various cell populations; including airway smooth muscle cells (45), eosinophils (46), and PBMC (47). In addition, dexamethasone treatment decreased MCP-1 mRNA expression and protein release in the peritoneal fluid in IL-1-stimulated mice (48).…”