Identification of PDL-1 as a novel biomarker of sensitizer exposure in dendritic-like cells

Abstract

We recommend you cite the published version. The publisher's URL is http://dx.doi.org/10.1016/j.tiv.2010.05.008Refereed: Yes (no note) Disclaimer UWE has obtained warranties from all depositors as to their title in the material deposited and as to their right to deposit such material. UWE makes no representation or warranties of commercial utility, title, or fitness for a particular purpose or any other warranty, express or implied in respect of any material deposited.UWE makes no representation that the use of the materials will not infringe any patent, copyright, trademark or other property or proprietary rights. UWE accepts no liability for any infringement of intellectual property rights in any material deposited but will remove such material from public view pending investigation in the event of an allegation of any such infringement. This is a PDF file of an unedited manuscript that has been accepted for publication. As a service to our customers we are providing this early version of the manuscript. The manuscript will undergo copyediting, typesetting, and review of the resulting proof before it is published in its final form. Please note that during the production process errors may be discovered which could affect the content, and all legal disclaimers that apply to the journal pertain. PLEASE SCROLL DOWN FOR TEXT. Accepted Manuscript ACCEPTED MANUSCRIPT ACCEPTED MANUSCRIPT Abstract.The and Cin. Our findings highlight novel biomarkers which, in MUTZ-3 cells, could be taken forward within a multiple biomarker in vitro assay ensuring strong and reliable predictability.

Ectosomes from neutrophil-like cells down-regulate nickel-induced dendritic cell maturation and promote Th2 polarization

“…We evidenced LPS-and NiSO 4 -induced PDL-1 up-regulation at the moDC surface, confirming data from a recent study of Langerhans cells [67]. These findings are consistent with other works, showing that some chemical sensitizers, such as cinnamaldehyde, eugenol, and isogenol, can also induce PDL-1 up-regulation at the DC surface [68]. Moreover, we found, for the first time, that PLB-Ect can decrease LPS-and NiSO 4 -induced PDL-1 up-regulation, suggesting that ectosomes might influence T cell activation and polarization during ACD.…”

Perspectives on Non-Animal Alternatives for Assessing Sensitization Potential in Allergic Contact Dermatitis

“…Further details of cell based priming assays elucidated in the literature 39,160 conclude that IL-8, 116,126 CD86, 119,153,165,168,169 and P38 MAP kinase 6,52,81,95–97,102,103,114,154 are the three commonly used metrics to distinguish sensitizer from irritant in various DC primary cells and cell line models.…”

In vitro models for immunogenicity prediction of therapeutic proteins

“…However, MUTZ-3 cell line was proven to be positive for antigens 525 HLA-A2, HLA-A3, HLA-B44, HLA-DR10, HLA-DR11, HLA-DR52, HLADQ5, and HLA-DQ7 [98]. More information on studies using the MUTZ-3 cell line model for detection of skin sensitizers [99][100][101][102][103][104], vaccine development [105], or to induce antitumor T cell immunity can be found in literature [106].…”