Expression of mRNAs of the Aquaporin Family in Mouse Oocytes and Embryos

Edashige, Keisuke; Sakamoto, Megumi; Kasai, Magosaburo

doi:10.1006/cryo.1999.2228

Cited by 72 publications

(54 citation statements)

References 16 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Actually, it has been shown that mRNAs of aquaporin 3 and aquaporin 7 are expressed in mouse oocytes and embryos [48], although the level of protein is not known. If such a channel actually affects permeation by glycerol, it might be reasonable to assume that the channel also transports ethylene glycol, a smaller polyhydric alcohol.…”

Section: Discussionmentioning

confidence: 99%

Permeability of Mouse Oocytes and Embryos at Various Developmental Stages to Five Cryoprotectants

Pedro

Yokoyama

Zhu

et al. 2005

Journal of Reproduction and Development

Self Cite

100

View full text Add to dashboard Cite

Abstract.To assess the permeability of mouse oocytes and embryos, matured oocytes and embryos at various stages of development were placed in five cryoprotectant solutions at 25 C for 25 min. From the cross-sectional areas of the oocytes/embryos, the relative change in volume was analyzed. In oocytes, shrinkage was least extensive and recovery was quickest in the propylene glycol solution, showing that propylene glycol permeates the oocytes most rapidly. Dimethyl sulfoxide, acetamide, and ethylene glycol permeated the oocytes slightly more slowly than propylene glycol. The oocytes in glycerol shrunk extensively and then expanded marginally, indicating slow permeation. The volume changes of 1-cell and 2-cell embryos were similar to those of oocytes, showing little change in permeability. In 8-cell embryos, the volume recovered much faster than in the earlier stages especially in glycerol and acetamide. In morulae, the volume recovery was much faster in glycerol and in ethylene glycol; in ethylene glycol, the extent of shrinkage was small and the recovery was fast, indicating an extremely rapid permeation. Although the permeability of oocytes/embryos generally increased as embryo development proceeded, the degree of increase varied greatly among the cryoprotectants. Interestingly, the volume change in propylene glycol was virtually unaffected by the stage of development. Such information will be valuable for determining a suitable protocol for the cryopreservation of oocytes/embryos at different stages of development. Key words: Mouse, Embryo, Oocyte, Permeability, Cryoprotectant (J. Reprod. Dev. 51: 235-246 , 2005) he survival of mammalian oocytes and embryos after cryopreservation varies with the stage of maturation and development. In general, oocytes/embryos at earlier stages appear to be more sensitive to cryopreservation. In the mouse, the rate of survival after cryopreservation is lower for unfertilized oocytes than embryos and increases as development proceeds up to the 8-cell or morula stage, although it may decrease as the blastocoel enlarges Cryoprotectants for mammalian oocytes and embryos are virtually limited to these five agents both in slow freezing and in vitrification [8].Although the protective action of cryoprotectants is considered colligative [9], each agent has its own specific properties. Among them, the permeating p r o p e r t y i s o f g r e a t i m p o r t a n c e , b e c a u s e permeation of the cell with a cryoprotectant is critical for the successful cryopreservation of

show abstract

Section: Discussionmentioning

confidence: 99%

Permeability of Mouse Oocytes and Embryos at Various Developmental Stages to Five Cryoprotectants

Pedro

Yokoyama

Zhu

et al. 2005

Journal of Reproduction and Development

Self Cite

100

View full text Add to dashboard Cite

show abstract

“…Expression of AQP1 gene has already been shown in different tissues and its role is to transport water through cell membrane (reviewed by Borgnia et al, 1999). In mice blastocysts, Offenberg & Thomsen (2005) and Edashige et al (2000) using the same primers but different mice strains did not found AQP1 transcripts, suggesting that AQP1 gene expression might differ among different strains. The current study has amplified mRNA in an attempt to ensure that minimal amounts of transcripts could be detected by Real-Time PCR, but it was also unable (Figure 1).…”

Section: Resultsmentioning

confidence: 98%

“…Blastocoel formation is essential for the preimplantation embryo development, and some aquaporins have been involved in this cavitation process (Barcroft et al, 2003;Edashige et al, 2000;Offenberg & Thomsen, 2005). Water and small solutes can easily flow across membrane but the presence of these aquaporin channels may facilitate the flow of liquids and cryoprotectors.…”

Section: Introductionmentioning

confidence: 99%

Relative expression of mRNAs related to cavitation process in bovine embryos produced in vivo and in vitro

et al. 2011

View full text Add to dashboard Cite

-The objectives of this work were to identify and to evaluate possible differences on gene expression of aquaporins and Na/K-ATPases transcripts between embryos in vivo and in vitro produced. For each group, 15 blastocysts distributed in three pools were used for RNA extraction followed by amplification and reverse transcription. The resulting cDNAs were submitted to Real-Time PCR, using the GAPDH gene as endogenous control. It was not possible to identify AQP1 transcripts. Relative expression of AQP3 (1.33 ± 0.78) and AQP11 (2.00 ± 1.42) were not different in blastocysts in vitro and in vivo produced. Na/K-ATPase α1 gene (2.25 ± 1.07) was overregulated whereas Na/K-ATPase β2 transcripts (0.40 ± 0.30) did not differ among blastocysts produced in vitro from those produced in vivo. Transcripts for gene AQP1 are not present in bovine blastocysts. In vitro culture system does not alter expression of genes AQP3, AQP11 and Na/K-ATPase β2 genes, however, it affects expression of Na/K-ATPase α1. encontrou-se sobrerregulado, enquanto o gene ATPase-Na/K β2 (0,40 ± 0,30) não diferiu entre os blastocistos produzidos in vitro e aqueles produzidos in vivo. Transcritos para o gene AQP1 não estão presentes em blastocistos bovinos. O sistema de cultivo in vitro não influencia a expressão dos genes AQP3, AQP11 e ATPase-Na/K β2, porém altera a expressão do gene ATPase-Na/K α1.Palavras-chave: blastocele, canal de água, expressão gênica, gradiente osmótico Revista Brasileira de Zootecnia

show abstract

“…The role of aquaporins (AQPs), water channel proteins involved in regulation of cellular water homeostasis, was studied in sea urchin, mouse, and human embryos, demonstrating their expression and implication in mammalian preimplantation embryo development (Edashige et al, 2000;Amaroli et al, 2013;Nong et al, 2013;Xiong et al, 2013). Members of the AQPs family take part in the processes of blastocyst formation as physiological mediators of fluid movement across the trophectoderm during cavitation and implantation (Cho et al, 2003;Richard et al, 2003;Watson et al, 2004).…”

Section: Mammalsmentioning

confidence: 99%

Ion currents in embryo development

Tosti

Boni

Gallo

2016

Birth Defects Research Pt C

View full text Add to dashboard Cite

Ion channels are proteins expressed in the plasma membrane of electrogenic cells. In the zygote and blastomeres of the developing embryo, electrical modifications result from ion currents that flow through these channels. This phenomenon implies that ion current activity exerts a specific developmental function, and plays a crucial role in signal transduction and the control of embryogenesis, from the early cleavage stages and during growth and development of the embryo. This review describes the involvement of ion currents in early embryo development, from marine invertebrates to human, focusing on the occurrence, modulation, and dynamic role of ion fluxes taking place on the zygote and blastomere plasma membrane, and at the intercellular communication between embryo cell stages.Birth Defects Research (Part C) 108:6-18, 2016.V C 2016 Wiley Periodicals, Inc.

show abstract

Expression of mRNAs of the Aquaporin Family in Mouse Oocytes and Embryos

Cited by 72 publications

References 16 publications

Permeability of Mouse Oocytes and Embryos at Various Developmental Stages to Five Cryoprotectants

Permeability of Mouse Oocytes and Embryos at Various Developmental Stages to Five Cryoprotectants

Relative expression of mRNAs related to cavitation process in bovine embryos produced in vivo and in vitro

Ion currents in embryo development

Contact Info

Product

Resources

About