Expression of MRP14 gene is frequently down-regulated in Chinese human esophageal cancer

Abstract

Migration inhibitory factor-related protein 14 (MRP14) is one of calcium-binding proteins, referred as S100A9. The heterodimeric molecule formed by MRP14 with its partner MRP8 (S100A8) is the major fatty acid carrier in neutrophils. The MRP8/14 complex has been also implicated in the intracellular transport of arachidonic acid and its precursors in keratinocytes. We show here the involvement of MRP14 in human esophageal cancer. In an initial study, mRNA differential display -reverse transcription polymerase chain reaction (DD-PCR) was performed with two esophageal carcinomas, one esophageal adenocarcinoma and matched normal adjacent mucosa. DD-PCR with the arbitrary primer OPA3 showed that one cDNA band was highly expressed in normal tissues, but disappeared or substantially decreased in tumor counterparts. It was later identified to be the 3'-end of migration inhibitory factor-related protein 14 (MRP14). Northern blotting, RT-PCR and Western blotting corroborated the down-regulation of MRP14 in 58/64 squamous cell carcinomas and 2/2 adenocarcinomas as compared with adjacent normal epithelia of the esophagus. MRP14 was undetectable in 3/3 esophageal-carcinoma cell lines. Immunochemistry demonstrated that expression of MRP14 was restricted to normal esophageal epithelia. No mutation was found in the genomic DNA of the MRP14 gene by PCR and directed DNA sequencing. Our finding suggested that the reduction of MRP14 expression is a frequent event in Chinese human esophageal cancer.

Identification of the up-regulation of TP-alpha, collagen alpha-1(VI) chain, and S100A9 in esophageal squamous cell carcinoma by a proteomic method

“…The present work indicates that protein S100A9 is up-regulated in 59.65% of the ESCC samples and down-regulated only in 10.17% of the ESCC cases. However, reduction of S100A9 expression is a frequent finding in Chinese human esophageal adenocarcinoma cancer [36,37]. This difference may arise from variations in the sample group, such as pathological typing and TNM stage, among others.…”

MicroRNA-196a Is a Potential Marker of Progression during Barrett's Metaplasia-Dysplasia-Invasive Adenocarcinoma Sequence in Esophagus

“…Although in squamous cell esophageal carcinomas, the loss of S100A8 and S100A9 is very well documented. 29 It has also been shown that the S100A9 levels correlate with the extent of tumor differentiation. 30 In a recent study, we have demonstrated that annexin A1, a potential tumor suppressor is an in vivo target of miR-196a in EA.…”

S100A8/A9 Induces Apoptosis and Inhibits Metastasis of CasKi Human Cervical Cancer Cells

“…They could resist microorganisms, induce apoptosis, and are the markers for various inflammatory diseases [10]. Recent studies demonstrate decreased expression of S100A8/A9 heterodimer in various squamous epithelial tumors (e.g., esophageal squamous carcinoma and head/neck squamous carcinoma) [11,12], consistent with a role of the heterodimer in the pathogenesis of squamous carcinomas. However, the expression of S100A8/A9 increases in various gland cell tumors and is associated with low differentiation of tumors [13,14].…”