Expression of MUC2 and MUC3 mRNA in human normal, malignant, and inflammatory intestinal tissues.

Abstract

MUCZ and MUC3 are prominent mucin genes expressed in the human intestine. Using in situ hybridization with RNA probes, we examined the cellular distribution of MUC2 and MUC3 mRNA in normal, malignant, and inflamma tory human intestinal tissues. In normal small intestine and colon, MUCZ mRNA was expressed exclusively in goblet cells and occurred throughout the entice height of the mucosa. MUC3 mRNA was expressed by goblet and columnar cells but was restricted to the villous com tment of the s m a l l intestine and MUC3 mRNA were both markedly decreased in poorly, moderately, and well-di&rentiated colon cancers but were preserved in mucinous colon cancers. In ulcerative colitis and Crohn's colitis tissues, MUC2 and MUC3 "A expression and the surface epithelium o r the colon. Expression of MUCZ

MUC13, a Novel Human Cell Surface Mucin Expressed by Epithelial and Hemopoietic Cells

“…Presence on the apical membrane of crypt epithelium mirrors the expression of two other cell surface mucins, MUC1 and MUC4, which also predominate in the crypts and are expressed by both major cell types (25). In contrast, MUC3 (26), and MUC12 2 appear to be most highly expressed by cells of the surface epithelium. MUC13 also appears to be secreted, as we have demonstrated its presence within goblet cell thecae and in secreted material in the large intestine and stomach, and within supranuclear vacuoles, but not on the apical membrane surface, of absorptive cells in the small intestine.…”

“…For example, although MUC1 and MUC13 appear to be expressed similarly in the colon (27) and in the stomach (28), staining of serial sections with a MUC1-reactive antibody revealed differing patterns of expression of MUC13 and MUC1 in gastric tissue (data not shown). As an additional example, in the surface epithelium MUC3 is expressed most highly in the small intestine and decreases distally with only low expression in the distal large intestine (26). MUC12 expression increases progressively from small intestine to rectum 2 ; similarly, MUC13 mRNA expression increases from small intestine to rectum.…”

Biomarker selection for detection of occult tumour cells in lymph nodes of colorectal cancer patients using real-time quantitative RT–PCR

“…Of all biomarkers CEA mRNA was expressed at the highest levels in tumours followed by CEACAM6. The high levels of these two markers could possibly be explained by the fact that CEA and CEACAM6 are expressed both in columnar epithelial cells and goblet cells in normal colon while CEACAM1 and CEACAM7 are expressed in columnar epithelial cells only and MUC2 in goblet cells only (Weiss et al, 1996;Frängsmyr et al, 1999). It can be argued that no or only marginal expression of the biomarker in immune cells should be of utmost importance for successful detection of tumour cells in lymph nodes.…”

“…Little is known about their relative expression levels and possible changes in CRC. Mucin 2 (MUC2) is the major mucin in colon (Weiss et al, 1996). It is a candidate tumour marker since its expression is restricted to epithelial cells.…”

Alterations in the Composition of the Supramucosal Defense Barrier in Relation to Disease Severity of Ulcerative Colitis

“…Alterations, however, in the expression of the mucin genes MUC1-4 have not been conclusively identified (Myerscough et al 1995;Weiss et al 1996). Sulfation of mucins appears to be reduced in UC (Corfield et al 1992(Corfield et al ,1996.…”