Expression of mucin-1 in oral squamous cell carcinoma and normal oral mucosa: An immunohistochemical study

Abstract

Background:Mucins are high-molecular-weight glycoproteins with a high O-linked carbohydrate content, which are synthesized by many secretory epithelial cells as membrane-bound and/or secreted products. Mucin-1 (MUC1) is a transmembrane mucin that protects and lubricates the mucous membranes of the human body and involves itself in various cellular functions such as growth, differentiation and signaling. An aberrant expression of MUC1 has been demonstrated in various human cancers. Many studies on MUC1 expression have been conducted on a variety of neoplastic lesions other than head-and-neck region. In this study, an attempt has been made to evaluate the MUC1 immunoexpression in oral squamous cell carcinoma (OSCC) and normal oral mucosa (NOM).Aims:This study aims to compare and correlate the immunoexpression of MUC1 in NOM and OSCC using immunohistochemical technique.Settings and Design:Thirty patients of OSCC formed the study group and thirty patients were included in the control group (NOM). Formalin-fixed paraffin wax blocks were prepared from the tissue samples obtained.Materials and Methods:Immunohistochemistry (IHC) for MUC1 was performed, and the overall percentage of positive cells along with distribution and localization of immunoexpression was studied.Statistical Analysis:Pearson's Chi-square test was used. P <0.05 was considered to be statistically significant.Results:In OSCC study group, MUC1-positive immunoreaction was observed in 21 (70%) cases out of 30. All the samples in control group were negative for MUC1 immunoexpression. The immunohistochemical expression of MUC1 in OSCC group was statistically significant when compared with normal control group, as P < 0.05 (Pearson's Chi-square).Conclusions:MUC1 is a reliable biomarker for the diagnosis of OSCC, but further studies are required to prove its role in prognosis.

Expression of Mucin1 in saliva in oral squamous cell carcinoma and oral potentially malignant disorders (case control study)

“…The strong association between MUC1 expression level in OPMDs in comparison with OSCC group may be possibly explained by involvement of other factors in the progression of the highly complex oral malignancy after the initial stage. The latter results are consistent with the results reported by other studies (Nitta et al, 2000;Thakur et al, 2018;Utispan & Koontongkaew, 2021) which reported no statistically significant difference of MUC1 expression between the different clinical stages of OSCC. However, positive associations between expression of MUC1/DF3 and clinicopathologic characteristics (tumor aggressiveness) were found in the study by Hamada et al (Hamada et al, 2012).…”

Optimization of an oral mucosa in vitro model based on cell line TR146

“…The expression of MUC1 in the buccal area and salivary glands has been described before. 57 - 59 MUC3 has shown to be upregulated in mucosal tissue (intestine) in a stress response, 60 but no data about expression in the oral mucosa has been described yet. MUC15 was shown to be expressed in explants of human oral mucosal epithelial cells.…”

N-acetylaspartate-Like Peak on MR Spectroscopy – A Useful Clue For Brain Metastases that Originate from Mucin- Expressing Primary?

“…In melanoma, mucin-conforiong amino acid threonine is affecting the metaststic activity and it might be reasonable to attribute the presence of the NAA-like peak observed on MRS in our two melanoma patients to this particular mucin component. Furthermore, a recent immunohistochemical study con rmed the presence of mucin-1 (MUC-1) in around 70% of oral squamous cell carcinomas [14].…”