Expression of Multiple Stem Cell Markers in Dental Pulp Cells Cultured in Serum-free Media

Hirata, Thais Miyuki; Ishkitiev, Nikolay; Yaegaki, Ken; Calenic, Bogdan; Ishikawa, Hiroshi; Nakahara, Taka; Mitev, Vanio; Tanaka, Tomoko; Haapasalo, Markus

doi:10.1016/j.joen.2010.03.002

Cited by 43 publications

(32 citation statements)

References 27 publications

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“…NANOG has been reported to be a key gene for maintaining pluripotency, as shown by the capacity for multilineage differentiation and perpetual selfrenewal of cells expressing this gene. Although DPPSCs share some features with other populations of stem cells in the tooth, they differ in other aspects, such as gene expression and differentiation potential (Hirata et al, 2010) due to their embryonic-like properties. In this work, we observed the formation of EB-like structures with characteristics similar to embryonic stem cells and MAPCs (Braccini et al, 2005;Verfaillie, 2005).…”

Section: Discussionmentioning

confidence: 99%

Dental Pulp of the Third Molar: A New Source of Pluripotent-like Stem Cells

Atari

Gil-Recio

Fabregat

et al. 2012

Journal of Cell Science

109

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SummaryDental pulp is particularly interesting in regenerative medicine because of the accessibility and differentiation potential of the tissue. Dental pulp has an early developmental origin with multi-lineage differentiation potential as a result of its development during childhood and adolescence. However, no study has previously identified the presence of stem cell populations with embryonic-like phenotypes in human dental pulp from the third molar. In the present work, we describe a new population of dental pulp pluripotent-like stem cells (DPPSCs) that were isolated by culture in medium containing LIF, EGF and PDGF. , and they show genetic stability in vitro based on genomic analysis with a newly described CGH technique. Interestingly, DPPSCs were able to form both embryoid-body-like structures (EBs) in vitro and teratoma-like structures that contained tissues derived from all three embryonic germ layers when injected in nude mice. We examined the capacity of DPPSCs to differentiate in vitro into tissues that have similar characteristics to mesoderm, endoderm and ectoderm layers in both 2D and 3D cultures. We performed a comparative RT-PCR analysis of GATA4, GATA6, MIXL1, NANOG, OCT3/4, SOX1 and SOX2 to determine the degree of similarity between DPPSCs, EBs and human induced pluripotent stem cells (hIPSCs). Our analysis revealed that DPPSCs, hIPSC and EBs have the same gene expression profile. Because DPPSCs can be derived from healthy human molars from patients of different sexes and ages, they represent an easily accessible source of stem cells, which opens a range of new possibilities for regenerative medicine.

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Section: Discussionmentioning

confidence: 99%

Dental Pulp of the Third Molar: A New Source of Pluripotent-like Stem Cells

Atari

Gil-Recio

Fabregat

et al. 2012

Journal of Cell Science

109

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“…It has also been reported that it is possible to culture deciduous dental pulp cells in serum-free medium 11) . STK2, which contains various protein growth factors and transferrin, is a culture medium that has been developed for mesenchymal stem cells.…”

Section: Introductionmentioning

confidence: 99%

Alterations in Deciduous Dental Pulp Cells Cultured with Serum-free Medium

Harada

Kawai

et al. 2015

J. Hard Tissue Biology.

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Human mesenchymal stem cells are capable of differentiating into various cell types and are useful for applications in regenerative medicine. Previous studies have indicated that stem cells can be isolated from dental pulp, and dental pulp exfoliated from deciduous teeth has become a useful alternative for dental tissue engineering because of its higher proliferation rate. For clinical application, it is necessary to culture cells in vitro and to obtain sufficient numbers of cells as quickly as possible. Furthermore, it is necessary to confirm the change in cells cultured in vitro in order to ensure that they have suitable characteristics. In this study, to investigate the changes in human deciduous dental pulp cells cultured with serum-free media, we analyzed cell proliferation, cell morphology and gene expression changes by microarray analysis. We found a high cell proliferation rate in cells that were cultured in STK2 that is a serum-free medium, and cells tended to remain in close contact with one another in cell morphology. In addition, 3248 genes were expressed at >2-fold higher levels in dental pulp cells from deciduous teeth cultured with STK2 compared with medium containing fetal bovine serum for 3 days.

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“…In regenerative medicine, we must lower the possibility of such an obstruction as much as possible. Therefore, we have recently developed a serum-free medium (SFM) for the differentiation of human DPSCs (13).…”

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confidence: 99%