Expression of mutant p53 in oral squamous cell carcinoma is correlated with the effectiveness of intra-arterial chemotherapy

Abstract

Abstract. The aim of the present study was to evaluate the correlation between the positive expression rate of mutant p53 and the clinical characteristics of patients with oral squamous cell carcinoma (OSCC), as well as the effectiveness of intra-arterial chemotherapy. Expression of mutant p53 in tumor tissues was determined by immunohistochemical analysis of 51 OSCC patients, prior to and following intra-arterial chemotherapy. Prior to intra-arterial chemotherapy, mutant p53 positive rates in patients with higher pathological grades were significantly higher than those of the patients with lower pathological grades. The mutant p53 positive rate in patients with lymph node metastasis was 73% (19/26), which was significantly higher than that of the patients without lymph node metastasis (20%, 5/25). Mutant p53 was expressed in 17% (3/18) of clinical phase II patients, while 64% (21/33) of clinical phase III and IV patients exhibited positive expression of mutant p53 (P<0.05). The mutant p53 positive rate in chemotherapy non-responsive patients was 69% (11/16), which was significantly higher than that in the chemotherapy-responsive patients (37%, 13/35). Mutant p53 positive rates were not significantly correlated with age, gender or the location of the tumor. The mutant p53 positive rate prior to chemotherapy was 47% (24/51), and decreased to 18% (9/51) following chemotherapy. Expression of mutant p53 was decreased in all 13 (100%) chemotherapy-responsive patients, while only 5 (45%) chemotherapy non-responsive patients exhibited reduced expression levels of mutant p53 (P<0.05). In conclusion, mutant p53 has a significant role in the differentiation, development and treatment guidance of OSCC. Intra-arterial chemotherapy with 5-fluorourcil and carboplatin potentially exerts a therapeutic effect by reducing the expression of mutant p53.

Can p53 expression and staining intensity correlate with histopathological prognostic parameter and clinical staging in head and neck squamous cell carcinoma?

“…Many researchers like Dragomir LP, Boslooper K, Geisler SA and Li Y had also concluded the same. 2,10,12,13 In the current study, p53 positivity was seen in 64% of the cases (percentage positive cells varying from 5-76% with mild, moderate and strong staining intensity and final score varying from 0 to 9 finds coherence with the work conducted by Franzman MB et al which has reported an immunoexpression of around 60percent. 8 The p53 positivity in cases of larynx and hypopharynx was relatively low (37%).…”

“…This positive correlation has been commented upon by other researchers as well. 9 The same correlation between p53 positivity and lymph node mets was found to be statistically significant in oral cavity and oropharyngeal cases in concordance with the work done by researchers like Jain A et al and Li Y et al 13,14 However, Takes RP et al records an opinion about this correlation which is diametrically opposite. 18 The significance of p53 in having a relationship with lymph node metastasis is the most important prognostic factor for overall survival of HNSCC as about two-third of the patients with HNSCC who present with lymph node metastasis have a poor five year survival rates of 40-50%.…”

PD-L1 expression correlated with p53 expression in oral squamous cell carcinoma

“…Mutated p53 loses its ability to suppress the function of oncogenes. Furthermore, mutant p53 may function as an oncogene to stimulate cell division and promote the growth of tumor cells [6]. .…”

Efficacy and Safety of Intro-Arterial Chemotherapy Combined with Radiotherapy on Head and Neck Cancer: A Systematic Review and Meta-Analysis

“…Other studies reported that mucositis and dysphagia were regarded to be inevitable but reversible 14, 32. Additionally, systemic toxicities such as anemia, thrombocytopenia and leukopenia were also reported before 60-62. Here, we summarized ten toxicities from the 34 studies, including oral diseases, mucositis, leukopenia, dermatitis, anemia, general condition, thrombocytopenia, nausea/vomiting, fever and hepatic dysfunction.…”