Expression of myogenic regulatory factor genes in skeletal muscle satellite cells from Wuzhumuqin sheep in vitro

Wu, Rihan; Yun, Yueying; Qinghui, Feng; Xia, Qin; He, Xige; Lu, Ciyong; Sha, Lei; Nishiumi, Tadayuki; Borjigin, Gerelt

doi:10.1016/j.smallrumres.2020.106251

Cited by 6 publications

(4 citation statements)

References 35 publications

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“…In livestock and poultry, myogenin is one of the important players in myogenesis and meat production [9,10]. Some studies [3,[42][43][44] reported that in early skeletal muscle satellite cells, myogenic factor 5 (MYF5) or myoblast determination protein (MYOD) is the first of the myogenic regulatory factors to be expressed while myogenin is expressed at later stages. Zhao et al [5] showed that myogenin expression in the breast muscles and in the leg muscles of geese had a positive association with body weight and concluded that myogenin can act as a mediator of muscle growth.…”

Section: Discussionmentioning

confidence: 99%

“…In order to perform each of these specific roles accurately, the expression of different genes must be controlled [2]. Myogenin is a protein in the myogenic regulatory factor family that plays an important role in determining carcass and meat traits and is vital for the growth and development of livestock muscles [3,4]. Myogenin is one of the most important regulatory factors associated with myoblast differentiation [5].…”

Section: Introductionmentioning

confidence: 99%

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An Origanum majorana Leaf Diet Influences Myogenin Gene Expression, Performance, and Carcass Characteristics in Lambs

Safaei

Dadpasand

Mohammadabadi

et al. 2022

Animals

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Myogenin is a protein in the myogenic regulatory factor family that plays an important role in determining carcass and meat traits and is vital for the growth and development of livestock muscles. The objective of this study was to determine the impact of Origanum majorana leaf (MOL) on the myogenin gene expression of lambs. Twenty-four male Kermani lambs were used in a completely randomized design using two experimental groups (0% Origanum majorana L. = MOL0 and 4% Origanum majorana L. = MOL4). Final weight, average daily gain, hot and cold carcass weight, feed conversion ratio, empty body weight, hot and cold dressing percentage, the weight of the shoulder, loin, leg, and lean meat, and the lean/bone ratio were measured. A standard kit was used for extracting total RNA from the loin, leg, and shoulder muscles of the lambs’ tissues. The cDNA was synthesized, a real-time PCR was performed, and the obtained data were analyzed. The results of this study showed that the effect of MOL4 on dry matter intake is not significant. The MOL4 diet increased final weight by 8.22%, average daily gain by 28.57%, hot carcass weight by 11.38%, cold carcass weight by 13.43%, feed conversion ratio by 31.03%, empty body weight by 9.38%, hot dressing percentage by 2.92%, cold dressing percentage by 3.75%, shoulder weight by 56.70%, loin weight by 8.98%, leg weight by 10.90%, lean meat weight by 14.62%, and the lean/bone ratio by 2.85% (p < 0.05) compared to the MOL0. Along with adding MOL4 in the lambs’ diets, in comparison with MOL0, there was higher expression of myogenin in the loin (3.5 times), leg (3.9 times), and shoulder (3.6 times) muscles of the lambs. Due to the fact that adding Origanum majorana to the diet of the lambs enhanced the expression of the myogenin gene in the loin, leg, and shoulder muscles and increased parameters related to growth, it can be used to improve the parameters related to growth and to increase myogenin gene expression in the muscle of lambs.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

An Origanum majorana Leaf Diet Influences Myogenin Gene Expression, Performance, and Carcass Characteristics in Lambs

Safaei

Dadpasand

Mohammadabadi

et al. 2022

Animals

View full text Add to dashboard Cite

show abstract

“…The fusion of myoblasts is required to produce multinucleated myo brils (Krauss et al, 2017) during the formation of skeletal muscles, called myogenesis, which play an active role in intranatal and postnatal growth (Braun and Gautel, 2011). The myogenic regulatory factor (MRF) gene family, which includes myogenin (MYOG), myoblast detection protein-1 (MYOD1), myogenic factor 5 (MYF5) and myogenic factor 6 (MYF6), has very important roles in the muscle growth and development (Cheng et al, 2020;Rihan et al, 2020). During this process, MYOD1, MYF5 and MRF6 control the formation of myogenic cells (Hernández-Hernández et al, 2017), while MYOG is required for terminal differentiation of skeletal muscle (Cheng et al, 2020).…”

Section: Introductionmentioning

confidence: 99%

“…The expression levels of the genes in the MRF family vary according to the developmental stage of the embryo in the intrauterine period (Rihan et al, 2020). For example, while the expression level of MYF5 is high in sheep fetuses in mid-pregnancy, the expression level of MYF6 is higher in the later stages of pregnancy (Quigley et al, 2014).…”

Section: Introductionmentioning

confidence: 99%

Investigation of Expression Levels of Transcription Factor Genes in Native Sheep Breeds of Turkey

Arslan

Daldaban

Bayram

et al. 2022

Preprint

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This study, it was aimed to investigate the expression levels of transcription factor (TF) genes (MYF6, MYOD1, MYF5, MYOG) and proteins associated with muscle growth in the longissimus dorsi (LD) and gluteal (GL) muscles in sheep. For this purpose, two fat-tailed sheep breeds (Akkaraman (n = 10), İvesi (n = 10)) and two thin-tailed sheep breeds (Kivircik (n = 10) and Karayaka (n = 10)) from Turkey's native sheep breeds were examined. The expression level of RNAs and proteins isolated from fresh tissues and MYF6, MYOD1, MYF5, MYOG proteins were analyzed. As a result of the statistical analysis, in the LD tissue, respectively, MYOG and MYF5 genes in the Karayaka sheep breed; MYOD1 gene in Akkaraman sheep breed; MYF5 gene in Awassi sheep breed were found to be statistically significant (P < 0.05). In GL tissue, respectively, MYOG and MYF6 genes in Akkaraman sheep breed; MYOD1 gene in Karayaka sheep breed; MYF6 gene in Akkaraman and Awassi sheep breed were found to be statistically significant (P < 0.05). In the present study, it was found that the MYOG (fold change 6.87) and MYOD1 (fold change 15.41) genes were upregulated in the GL muscle of the fat-tailed Akkaraman sheep breed. In addition, in the thin-tailed Karayaka sheep breed, down-regulation of MYOD1 (fold change − 0.22) gene in LD muscle and up-regulation of MYOD1 (fold change 6.67) gene in GL muscle was found. As a result, it can be considered that MYOG and MYOD1 genes as candidate genes in molecular selection studies for our fat-tailed and thin-tailed indigenous breeds in terms of muscle development.

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Single nucleotide polymorphisms in the MRFs gene family associated with growth in Nile tilapia

Wei,

Xiao,

Chen

et al. 2024

Mol Biol Rep

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Expression of myogenic regulatory factor genes in skeletal muscle satellite cells from Wuzhumuqin sheep in vitro

Cited by 6 publications

References 35 publications

An Origanum majorana Leaf Diet Influences Myogenin Gene Expression, Performance, and Carcass Characteristics in Lambs

An Origanum majorana Leaf Diet Influences Myogenin Gene Expression, Performance, and Carcass Characteristics in Lambs

Investigation of Expression Levels of Transcription Factor Genes in Native Sheep Breeds of Turkey

Single nucleotide polymorphisms in the MRFs gene family associated with growth in Nile tilapia

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