Expression of N‐cadherin mRNA during development of the mouse brain

Redies, Christoph; Takeichi, Masatoshi

doi:10.1002/aja.1001970104

Cited by 109 publications

(66 citation statements)

References 51 publications

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“…The observation that CDO associated with N-cadherin in proliferating myoblasts and throughout differentiation while it associated with M-cadherin only transiently during differentiation suggests that functional distinctions between these interactions may also exist. Finally, it is worth noting that N-cadherin, CDO, and BOC are coexpressed in additional cell types during development such as neural precursors and precartilaginous, condensing mesenchymal cells (18,19,25,26). The interactions described here may play a developmental role in these cell types as well.…”

Section: Discussionmentioning

confidence: 74%

Promyogenic members of the Ig and cadherin families associate to positively regulate differentiation

Kang

Feinleib

Knox

et al. 2003

Proc. Natl. Acad. Sci. U.S.A.

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Determination and differentiation of cells in the skeletal muscle lineage is positively regulated by cell-cell contact. Cell-surface proteins proposed to mediate this effect include both classical cadherins and Ig superfamily members; potential interactions between the promyogenic activities of these classes of protein, however, are unknown. We show here that CDO and BOC, two promyogenic Ig superfamily members that bind to each other in a cis fashion, form complexes with N-and M-cadherin. These complexes contain ␤-catenin and are enriched at sites of cell-cell contact between myoblasts. In transient expression assays, the ectodomains and intracellular regions of CDO, BOC, and N-cadherin each interact independently, suggesting that the interactions occur in a cis fashion; consistent with this conclusion, cadherin-mediated cell adhesion is not required for them to occur. Stable expression in myoblasts of a CDO deletion mutant deficient in its ability to associate with N-cadherin interferes with differentiation as assessed by biochemical, morphological, and reporter gene assays, suggesting that this interaction is functionally important in myogenesis. Thus, some of the cell-cell contact-mediated activities that are required for myogenesis seem to be based on interdependent activities of promyogenic classical cadherins and Ig superfamily members. Many types of cell-cell communication require the formation of intimate intercellular contacts (1). Cadherins play a key role in mediating such phenomena, because they are centrally involved in establishing cell-cell adhesive structures. Furthermore, cadherin-based adhesion can activate signaling pathways via two nonmutually exclusive mechanisms: (i) control of the ability of juxtacrine signaling ligands and receptors to make intercellular connections and (ii) direct regulation of signaling processes (1, 2). Among the numerous cellular activities that are regulated by cadherins is cell differentiation (1).Skeletal myogenesis is viewed as a paradigm for understanding the molecular events that link cell-lineage determination, cell differentiation, and tissue-specific gene expression (3, 4). Interestingly, determination and differentiation of cells in the skeletal muscle lineage is positively regulated by cell-cell contact (5-7). Several cadherins have been implicated in this process including N-, M-, and R-cadherin. N-cadherin has been studied most extensively; several lines of evidence indicate that it plays a positive role in skeletal myogenesis. Expression of a dominantnegative form of N-cadherin in early Xenopus laevis embryos suppresses expression of the myogenic transcription factor, MyoD, in muscle progenitor cells (8). Furthermore, several different function-perturbing antibodies to N-cadherin inhibit myogenic differentiation in vitro of chick primitive streak epiblast cells (9), primary chicken embryo myoblasts (10), and C2C12 murine myoblasts (11). Conversely, incubation of C2C12 and other myoblast cell lines with beads coated with recombinant N-cadherin extracellul...

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Section: Discussionmentioning

confidence: 74%

Promyogenic members of the Ig and cadherin families associate to positively regulate differentiation

Kang

Feinleib

Knox

et al. 2003

Proc. Natl. Acad. Sci. U.S.A.

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“…The expression pattern of many cadherins has been intensively studied during brain development by in situ hybridization especially in the chicken and in the mouse brain. The majority of cadherins investigated is expressed in a spatially restricted manner in fi ber tracts, brain nuclei, and neural circuits during different stages of development (Redies, 2000;Redies and Takeichi, 1993;Simonneau and Thiery, 1998;Suzuki et al, 1997;Kimura et al, 1996). Whereas cadherin mRNA localization has been mapped in detail previously, in this study we quantifi ed mRNA levels of cadherins in various regions of the adult rat brain, to our knowledge, for the fi rst time.…”

Section: Discussionmentioning

confidence: 90%

Different Regulation of N-Cadherin and Cadherin-11 in Rat Hippocampus

Bartelt-Kirbach

Langer-Fischer

Golenhofen

2010

Cell Communication & Adhesion

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Cadherin-mediated specifi c cell adhesion is an important process in brain development as well as in synaptic plasticity in the adult brain. In this study the authors quantifi ed mRNA levels of N-cadherin and cadherin-11 in different brain regions for the fi rst time. In hippocampus N-cadherin mRNA levels were very high at embryonic stages and decreased during further development, whereas cadherin-11 mRNA levels were highest at postnatal stages. However, N-cadherin protein level was not altered during hippocampal development and cadherin-11 protein was low at embryonic but high at postnatal and adult stages. In cultured hippocampal neurons both cadherins became colocalized and recruited to synaptic sites during ongoing differentiation, with especially high accumulation of cadherin-11 at synapses. These data hint at a critical role of N-cadherin at early embryonic stages and early synaptogenesis, whereas cadherin-11 might be more important for further stabilization of synapses in the postnatal period and adulthood.

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“…N-cadherin mRNA is selectively expressed in deep-layer neurons, and specifically during the perinatal period when deep and superficial neurons are segregating (Redies and Takeichi, 1993). We suggest that the reeler mutation may affect an intrinsic adhesion program in cortical neurons by hypermorphic regulation of an adhesive factor that is peculiar to deep-layer neurons such as N-cadherin.…”

Section: Discussionmentioning

confidence: 94%

The mouse mutation reeler causes increased adhesion within a subpopulation of early postmitotic cortical neurons

et al. 1995

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Early postmitotic cortical neurons are mostly corticofugal projection neurons that take up positions in deep cortical laminae. Later postmitotic neurons are preferentially localized to superficial cortical laminae. In reeler mutant mice it appears that cortical laminar positions with respect to birthdate are reversed (Caviness, 1982). In a reanalysis of reeler lamination we found that early postmitotic cortical neurons labeled by embryonic day (E) 11–13 injections of a birthdate marker, or by early postnatal day (PND) 2 retrograde labeling through their output projections, appear to take up positions both in the superficial and deep cortex. Neurons born on E11 and E12 are more likely to be situated superficially in the reeler cortex and neurons born on E13 are more likely to be situated in the deep reeler cortex. Many corticofugal projection neurons in the deep (but not superficial) reeler cortex either die or retract their axons before PND 21. We hypothesize that the earliest postmitotic (E11-E12) of the early postmitotic reeler cortical neurons are overly adhesive and act as a barrier to later postmitotic migrating neurons. In vitro cortical aggregation cultures confirmed that early postmitotic (E12) reeler neurons are more adhesive than early postmitotic (E12) wild-type neurons or late postmitotic (E16) reeler or wild-type cortical neurons. We suggest that the moderate wild-type preferential adhesion of early postmitotic cortical neurons to each other helps deep and superficially fated lineages to form cortical laminae.

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Expression of N‐cadherin mRNA during development of the mouse brain

Cited by 109 publications

References 51 publications

Promyogenic members of the Ig and cadherin families associate to positively regulate differentiation

Promyogenic members of the Ig and cadherin families associate to positively regulate differentiation

Different Regulation of N-Cadherin and Cadherin-11 in Rat Hippocampus

The mouse mutation reeler causes increased adhesion within a subpopulation of early postmitotic cortical neurons

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