Expression of NADPH oxidase homologues and accessory genes in human cancer cell lines, tumours and adjacent normal tissues

Juhász, Ágnes; Ge, Yun; Markel, Susan; Chiu, Alice; Matsumoto, Linda; Balgooy, Josephus van; Roy, Krishnendu; Doroshow, James H.

doi:10.1080/10715760902918683

Cited by 135 publications

(169 citation statements)

References 37 publications

(43 reference statements)

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“…38,39 Moreover, a microarray analysis indicated that Noxo1 expression is upregulated in a subpopulation of colon cancers. 40 Accordingly, it is possible that Noxo1 is induced in cancer cells, together with NOX1, in the inflammatory microenvironment, contributing to an oncogeneinduced transformation phenotype through the generation of reactive oxygen species.…”

Section: Discussionmentioning

confidence: 99%

TNF-α/TNFR1 signaling promotes gastric tumorigenesis through induction of Noxo1 and Gna14 in tumor cells

et al. 2013

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Helicobacter pylori infection induces chronic inflammation that contributes to gastric tumorigenesis. Tumor necrosis factor (TNF-a) is a proinflammatory cytokine, and polymorphism in the TNF-a gene increases the risk of gastric cancer. We herein investigated the role of TNF-a in gastric tumorigenesis using Gan mouse model, which recapitulates human gastric cancer development. We crossed Gan mice with TNF-a (Tnf) or TNF-a receptor TNFR1 (Tnfrsf1a) knockout mice to generate Tnf À / À Gan and Tnfrsf1a À / À Gan mice, respectively, and examined their tumor phenotypes. Notably, both Tnf À / À Gan mice and Tnfrsf1a À / À Gan mice showed similar, significant suppression of gastric tumor growth compared with control Tnf þ / þ or Tnfrsf1a þ / þ Gan mice. These results indicate that TNF-a signaling through TNFR1 is important for gastric tumor development. Bone marrow (BM) transplantation experiments showed that TNF-a expressed by BM-derived cells (BMDCs) stimulates the TNFR1 on BMDCs by an autocrine or paracrine manner, which is important for gastric tumor promotion. Moreover, the microarray analysis and colony formation assay indicated that NADPH oxidase organizer 1 (Noxo1) and Gna14 are induced in tumor epithelial cells in a TNF-a-dependent manner, and have an important role in tumorigenicity and tumor-initiating cell property of gastric cancer cells. Accordingly, it is possible that the activation of TNF-a/TNFR1 signaling in the tumor microenvironment promotes gastric tumor development through induction of Noxo1 and Gna14, which contribute to maintaining the tumor cells in an undifferentiated state. The present results indicate that targeting the TNF-a/TNFR1 pathway may be an effective preventive or therapeutic strategy for gastric cancer.

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Section: Discussionmentioning

confidence: 99%

TNF-α/TNFR1 signaling promotes gastric tumorigenesis through induction of Noxo1 and Gna14 in tumor cells

et al. 2013

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“…Although at high concentrations ROS can be cytotoxic, physiologic ROS levels play a critical role in the control of tumor cell growth, differentiation, and signal transduction (7). Although ROS are primarily a by-product of aerobic metabolism in mammalian cells, the recently discovered family of epithelial NADPH oxidases (reduced nicotinamide adenine dinucleotide phosphate oxidases (Noxs)), contributes significantly to intracellular ROS production in tumor cells (8,9). Dual oxidase 2 (Duox2) belongs to the Nox family of oxidases that is composed of six additional enzymes, including Nox1-5 and dual oxidase 1 (Duox1) (10).…”

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confidence: 99%

Up-regulation and Sustained Activation of Stat1 Are Essential for Interferon-γ (IFN-γ)-induced Dual Oxidase 2 (Duox2) and Dual Oxidase A2 (DuoxA2) Expression in Human Pancreatic Cancer Cell Lines

Antony

Juhász

et al. 2011

Journal of Biological Chemistry

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“…Elevated levels of lipid rafts have been reported in breast cancer cells (27). Recently, expression of Nox isoforms (Nox1-5) has been reported in some breast cancer cell lines, including MCF-7 (46). Therefore, we speculated that there is an association between lipid rafts and NADPH oxidases in breast cancer cells.…”

Section: Discussionmentioning

confidence: 95%

Regulation of NADPH oxidase (Nox2) by lipid rafts in breast carcinoma cells

Malla

Raghu²,

Rao³

2010

Int J Oncol

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Abstract. Oxidative stress has emerged as an important pathogenic factor in the development of breast cancer. Cholesterol-rich membrane rafts or lipid rafts (LRs) are reported to play an important role in oxidative stress-induced signal transduction. NADPH oxidase-dependent reactive oxygen species (ROS) production is implicated in oxidative stress in human mammary epithelial cells. In the present study, we determined the expression and regulation of membranebound subunits by LRs in human breast cancer cells. We report that basal levels of gp91 phox and p22 phox are expressed in breast cancer cells. We demonstrate for the first time that disruption of LRs resulted in the downregulation of NADPH oxidase subunits in breast cancer cells. Cholesterol depletion by 10 mM methyl-ß-cyclodextrin (MßCD) translocated both gp91 phox and p22 phox out of LRs. Moreover, lipid raft disruption decreased NADPH oxidase activity (21.1±0.5% in MCF-7 and 28.9±1.0 in BT-549 cells), which was reversed by cholesterol repletion (95%). Therefore, the results suggest that the integrity of LRs plays an important role in the regulation of NADPH oxidase activity in breast cancer cells.

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Expression of NADPH oxidase homologues and accessory genes in human cancer cell lines, tumours and adjacent normal tissues

Cited by 135 publications

References 37 publications

TNF-α/TNFR1 signaling promotes gastric tumorigenesis through induction of Noxo1 and Gna14 in tumor cells

TNF-α/TNFR1 signaling promotes gastric tumorigenesis through induction of Noxo1 and Gna14 in tumor cells

Up-regulation and Sustained Activation of Stat1 Are Essential for Interferon-γ (IFN-γ)-induced Dual Oxidase 2 (Duox2) and Dual Oxidase A2 (DuoxA2) Expression in Human Pancreatic Cancer Cell Lines

Regulation of NADPH oxidase (Nox2) by lipid rafts in breast carcinoma cells

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