Expression of neurotrophin receptor trkB in rat cochlear hair cells at time of rearrangement of innervation

Knipper, Marlies; Zimmermann, Ulrike; Rohbock, Karin; Köpschall, Iris; Zenner, Hans‐Peter

doi:10.1007/s004410050545

Cited by 48 publications

(48 citation statements)

References 70 publications

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“…Interestingly p75 NTR overexpression rescued this phenotype, suggesting the presence of a cross-linked system (Michaelsen et al, 2010). In the cochlea p75 NTR is expressed in cochlear Schwann Cells (Provenzano et al, 2011) and full-length and truncated TrkB isoforms are expressed at the level of IHCs as well as SG (Knipper et al, 1996). In p75 KO mice however, SG survival is compromised after noise, suggesting p75 NTR mediates a rather protecting role (Tan et al, 2010).…”

Section: Ko Micementioning

confidence: 97%

Lack of Brain-Derived Neurotrophic Factor Hampers Inner Hair Cell Synapse Physiology, But Protects against Noise-Induced Hearing Loss

Zuccotti¹,

Kuhn²,

Johnson³

et al. 2012

Journal of Neuroscience

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120

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The precision of sound information transmitted to the brain depends on the transfer characteristics of the inner hair cell (IHC) ribbon synapse and its multiple contacting auditory fibers. We found that brain derived neurotrophic factor (BDNF) differentially influences IHC characteristics in the intact and injured cochlea. Using conditional knock-out mice (BDNF Pax2 KO) we found that resting membrane potentials, membrane capacitance and resting linear leak conductance of adult BDNF Pax2 KO IHCs showed a normal maturation. Likewise, in BDNF Pax2 KO membrane capacitance (⌬C m ) as a function of inward calcium current (I Ca ) follows the linear relationship typical for normal adult IHCs. In contrast the maximal ⌬C m , but not the maximal size of the calcium current, was significantly reduced by 45% in basal but not in apical cochlear turns in BDNF Pax2 KO IHCs. Maximal ⌬C m correlated with a loss of IHC ribbons in these cochlear turns and a reduced activity of the auditory nerve (auditory brainstem response wave I). Remarkably, a noise-induced loss of IHC ribbons, followed by reduced activity of the auditory nerve and reduced centrally generated wave II and III observed in control mice, was prevented in equally noise-exposed BDNF Pax2 KO mice. Data suggest that BDNF expressed in the cochlea is essential for maintenance of adult IHC transmitter release sites and that BDNF upholds opposing afferents in high-frequency turns and scales them down following noise exposure.

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Section: Ko Micementioning

confidence: 97%

Lack of Brain-Derived Neurotrophic Factor Hampers Inner Hair Cell Synapse Physiology, But Protects against Noise-Induced Hearing Loss

Zuccotti¹,

Kuhn²,

Johnson³

et al. 2012

Journal of Neuroscience

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120

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“…We used these low concentrations to activate specifically the high-affinity cognate receptors for BDN F and N T-3, trkB, and trkC, respectively (Pirvola et al, 1992(Pirvola et al, , 1994Ylikoski et al, 1993;Schecterson and Bothwell, 1994;Knipper et al, 1996;Mou et al, 1997;Cochran et al, 1999;Farinas et al, 2001). To confirm that these low concentrations were not also activating the low-affinity, nonspecific p75 N TR receptor we also exposed a series of cultures to 5 ng /ml of nerve growth factor (NGF; Preprotech).…”

Section: Methodsmentioning

confidence: 99%

“…To determine whether these electrophysiological features are subject to extrinsic regulation, we evaluated the effects of neurotrophins on neuronal firing patterns and on ion channel distribution of postnatal spiral ganglion neurons placed in tissue culture. We used brain-derived neurotrophic factor (BDNF) and neurotrophin-3 (NT-3), which act via high-affinity tyrosine kinase receptors, trkB and trkC, respectively (Barbacid, 1994;Patapoutian and Reichardt, 2001), and are known to be present in the peripheral auditory system (Pirvola et al, 1992(Pirvola et al, , 1994Ylikoski et al, 1993;Knipper et al, 1996;Mou et al, 1997;Cochran et al, 1999;Farinas et al, 2001). To address the issue of cell specificity, neurons from the apex (low frequency region) and base (high frequency region) of the cochlea were studied separately.…”

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confidence: 99%

Opposite Actions of Brain-Derived Neurotrophic Factor and Neurotrophin-3 on Firing Features and Ion Channel Composition of Murine Spiral Ganglion Neurons

2002

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It is now well established that sensory neurons and receptors display characteristic morphological and electrophysiological properties tailored to their functions. This is especially evident in the auditory system, where cells are arranged tonotopically and are highly specialized for precise coding of frequency-and timing-dependent auditory information. Less well understood, however, are the mechanisms that give rise to these biophysical properties. We have provided insight into this issue by using whole-cell current-clamp recordings and immunocytochemistry to show that BDNF and NT-3, neurotrophins found normally in the cochlea, have profound effects on the firing properties and ion channel distribution of spiral ganglion neurons in the murine cochlea. Exposure of neurons to BDNF caused all neurons, regardless of their original cochlear position, to display characteristics of the basal neurons. Conversely, NT-3 caused cells to show the properties of apical neurons. These results are consistent with oppositely oriented gradients of these two neurotrophins and/or their high-affinity receptors along the tonotopic map, and they suggest that a combination of neurotrophins are necessary to establish the characteristic firing features of postnatal spiral ganglion neurons.

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“…The elevated levels of TrkB, TrkC and p75NTR could be significant in ensuring the survival of maturing inner ear neurons. TrkB could also be critical for a later rearrangement of innervation as observed in murines (Knipper et al 1996), while both TrkC and TrkB upregulation could be significant in the normal development of the afferent innervation processes as observed in murines (Fritzsch et al 1999).…”

Section: Discussionmentioning

confidence: 99%

Role of BDNF and neurotrophic receptors in human inner ear development

et al. 2017

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The expression patterns of the neurotrophin, brain-derived neurotrophic factor, BDNF, and the neurotrophic receptors-p75NTR and Trk receptors-in the developing human fetal inner ear between the gestational weeks (GW) 9 to 12 are examined via in situ hybridization and immunohistochemistry. BDNF mRNA expression was highest in the cochlea at GW 9 but declined in the course of development. In contrast to embryonic murine specimens, a decline in BDNF expression from the apical to the basal turn of the cochlea could not be observed. p75NTR immunostaining was most prominent in the nerve fibers that penetrate into the sensory epithelia of the cochlea, the urticule and the saccule as gestational age progresses. TrkB and TrkC expression intensified towards GW 12, at which point the BDNF mRNA localization was at its lowest. TrkA expression was limited to fiber subpopulations of the facial nerve at GW 10. In the adult human inner ear, we observed BDNF mRNA expression in the apical poles of the cochlear hair cells and supporting cells, while in the adult human utricle, the expression was localized in the vestibular hair cells. We demonstrate the highly specific staining patterns of BDNF mRNA and its putative receptors over a developmental period in which multiple hearing disorders are manifested. Our findings suggest that BDNF and neurotrophin receptors are important players during early human inner ear development. In particular, they seem to be important for the survival of the afferent sensory neurons.

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Expression of neurotrophin receptor trkB in rat cochlear hair cells at time of rearrangement of innervation

Cited by 48 publications

References 70 publications

Lack of Brain-Derived Neurotrophic Factor Hampers Inner Hair Cell Synapse Physiology, But Protects against Noise-Induced Hearing Loss

Lack of Brain-Derived Neurotrophic Factor Hampers Inner Hair Cell Synapse Physiology, But Protects against Noise-Induced Hearing Loss

Opposite Actions of Brain-Derived Neurotrophic Factor and Neurotrophin-3 on Firing Features and Ion Channel Composition of Murine Spiral Ganglion Neurons

Role of BDNF and neurotrophic receptors in human inner ear development

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