Expression of osa-miR7695 against the blast fungus Magnaporthe oryzae in Vietnamese rice cultivars

Quốc, Nguyễn Bảo; Phuong, Nguyen Doan Nguyen; Trang, Ho Thi Thu; Phi, Nguyen Bang; Châu, Nguyễn Ngọc

doi:10.1007/s10658-019-01772-5

Cited by 10 publications

(9 citation statements)

References 34 publications

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“…On the other hand, a subset of MIR genes may be down-or up-regulated during PTI and/or ETI, depending on their roles as negative or positive regulators of PTI and/or ETI, whereas the positive regulators would be up-regulated during PTI and/or ETI, the negative regulators would be down-regulated. Evidence supporting this conclusion comes from functionally characterized miRNAs, including miR160, miR164, miR166, miR167, miR169, miR319, miR396, miR398, miR444 and miR7695 (Campo et al 2013;Li et al 2014;Li et al 2017;Xiao et al 2017;Wang et al 2018a;Zhang et al 2018;Chandran et al 2019;Quoc et al 2019;Zhao et al 2019). However, we still do not know how PTI and/or ETI is coupled with the miRNA signaling pathway or with each of the blast fungus-responsive miRNAs.…”

Section: Discussionmentioning

confidence: 99%

“…miR7695 seems to occur specifically in rice, particularly in the japonica subspecies and in some indica accessions. Its expression is higher in blast-resistant accessions than in blast-susceptible ones, and is further upregulated in response to infection by M. oryzae (Quoc et al 2019). Overexpression of its precursor leads to increased resistance to M. oryzae.…”

Section: The Micrornas That Play Positive Roles In Rice Immunity Agaimentioning

confidence: 99%

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The roles of rice microRNAs in rice-Magnaporthe oryzae interaction

Jeyakumar

Feng

et al. 2019

Phytopathol Res

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MicroRNAs (miRNAs) are a class of small (20-24 nucleotides (nt) long) non-coding RNAs. One mature miRNA can be transcribed from one or more gene loci known as miRNA genes (MIRs). The transcript of a MIR forms a stem-loop structure that is processed into a 20-24-nt miRNA-5p/−3p duplex by RNase III family endoribonucleases such as Dicer-like1 (DCL1). In turn, the overhang ends of the duplex are methylated by HUA ENHANCER 1 (HEN1), generating stabilized mature miRNAs. The mature miRNAs are loaded onto ARGONAUTE (AGO) proteins, forming a miRNAinduced gene silencing complex (miRISC). Then, the miRISC binds to target sites with sequences complementary to the miRNAs, leading to either cleavage or translational inhibition of the target mRNAs, or methylation of the target sequences, resulting in post-transcriptional and transcriptional gene silencing, respectively. In the past decade, more than 700 miRNAs have been identified in rice, a subset of which have been found to be responsive to the rice blast fungus, Magnaporthe oryzae, or its elicitors. Moreover, members of 10 miRNA families have been found to positively or negatively regulate rice defense against M. oryzae, namely miR160, miR164, miR166, miR167, miR169, miR319, miR396, miR398, miR444 and miR7695. This review summarizes the identification and functional characterization of the miRNAs, which respond to M. oryzae or its elicitors and describes the current understanding of the complicated but wellorganized network in the context of rice-M. oryzae interaction.

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Section: Discussionmentioning

confidence: 99%

Section: The Micrornas That Play Positive Roles In Rice Immunity Agaimentioning

confidence: 99%

The roles of rice microRNAs in rice-Magnaporthe oryzae interaction

Jeyakumar

Feng

et al. 2019

Phytopathol Res

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“…In rice, over 700 mature miRNAs have been identified in the genome (Tang and Chu, 2017 ) and at least 70 miRNAs respond to Magnaporthe oryzae (M. oryzae) infection, meaning that many miRNAs are involved in rice immunity (Li et al., 2014 ). Among them, 16 miRNAs have been characterized as fine regulators affecting rice blast resistance (Feng et al., 2021 ; Wang et al., 2021 ), as positive regulators, such as Osa‐miR1320, Osa‐miR160a, Osa‐miR162a, Osa‐miR166k‐h, Osa‐miR398b, and Osa‐miR7695 (Li et al., 2014 ; Li, Jeyakumar, et al., 2019b ; Quoc et al., 2019 ; Salvador‐Guirao et al., 2018 ; Sánchez‐Sanuy et al., 2019 ; Wang et al., 2021 ), or as negative regulators, such as Osa‐miR156, Osa‐miR164a, Osa‐miR167d, Osa‐miR168, Osa‐miR169a, Osa‐miR319b, Osa‐miR396, Osa‐miR444b.2, Osa‐miR1873, and Osa‐miR535 (Chandran et al., 2019 ; Li et al., 2017 ; Wang et al., 2016 , 2018 , 2021 ; Zhang et al., 2018 , 2020 ; Zhao et al., 2019 ; Zhou et al., 2019 ). Further investigation revealed that Osa‐miR535 targets the SQUAMOSA Promoter Binding Protein Like 14 ( OsSPL14 ) (Wang et al., 2021 ).…”

Section: Introductionmentioning

confidence: 99%

Osa‐miR535 targets SQUAMOSApromoter binding protein‐like 4 to regulate blast disease resistance in rice

et al. 2022

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SUMMARY Many rice microRNAs have been identified as fine‐tuning factors in the regulation of agronomic traits and immunity. Among them, Osa‐miR535 targets SQUAMOSA promoter binding protein‐like 14 (OsSPL14) to positively regulate tillers but negatively regulate yield and immunity. Here, we uncovered that Osa‐miR535 targets another SPL gene, OsSPL4, to suppress rice immunity against Magnaporthe oryzae. Overexpression of Osa‐miR535 significantly decreased the accumulation of the fusion protein SPL4TBS‐YFP that contains the target site of Osa‐miR535 in OsSPL4. Consistently, Osa‐miR535 mediated the cleavage of OsSPL4 mRNA between the 10th and 11th base pair of the predicted binding site at the 3′ untranslated region. Transgenic rice lines overexpressing OsSPL4 (OXSPL4) displayed enhanced blast disease resistance accompanied by enhanced immune responses, including increased expression of defense‐relative genes and up‐accumulated H2O2. By contrast, the knockout mutant osspl4 exhibited susceptibility. Moreover, OsSPL4 binds to the promoter of GH3.2, an indole‐3‐acetic acid‐amido synthetase, and promotes its expression. Together, these data indicate that Os‐miR535 targets OsSPL4 and OsSPL4‐GH3.2, which may parallel the OsSPL14‐WRKY45 module in rice blast disease resistance.

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“…For example, overexpression of miR1873 enhanced the susceptibility of rice to M. oryzae by regulating its target gene LOC_Os05g01790 (Zhou et al 2020 ). In addition, miR396 , miR169 , miR164a , miR319b , and miR167d negatively regulate immunity against M. oryzae in rice (Li et al 2017a ; Wang et al 2018 ; Zhang et al 2018 ; Chandran et al 2019 ; Zhao et al 2019 ), whereas miR398b , miR160a , miR166k-miR166h , miR7695 , and miR162a positively regulate the response against M. oryzae in rice (Achard et al 2004 ; Salvador-Guirao et al 2018 ; Li et al 2019a ; Li et al 2019b ; Quoc et al 2019 ; Li et al 2020a ).…”

Section: Introductionmentioning

confidence: 99%

Fine-Tuning Roles of Osa-miR159a in Rice Immunity Against Magnaporthe oryzae and Development

Chen

et al. 2021

Rice

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Background Rice blast caused by Magnaporthe oryzae is one of the most destructive diseases of rice. An increasing number of microRNAs (miRNAs) have been reported to fine-tune rice immunity against M. oryzae and coordinate with growth and development. Results Here, we showed that rice microRNA159a (Osa-miR159a) played a positive role in rice resistance to M. oryzae. The expression of Osa-miR159a was suppressed in a susceptible accession at 12, 24, and 48 h post-inoculation (hpi); it was upregulated in a resistant accession of M. oryzae at 24 hpi. The transgenic rice lines overexpressing Osa-miR159a were highly resistant to M. oryzae. In contrast, the transgenic lines expressing a short tandem target mimic (STTM) to block Osa-miR159a showed enhanced susceptibility. Knockout mutations of the target genes of Osa-miR159a, including OsGAMYB, OsGAMYBL, and OsZF, led to resistance to M. oryzae. Alteration of the expression of Osa-miR159a impacted yield traits including pollen and grain development. Conclusions Our results indicated that Osa-miR159a positively regulated rice immunity against M. oryzae by downregulating its target genes. Proper expression of Osa-miR159a was critical for coordinating rice blast resistance with grain development.

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Expression of osa-miR7695 against the blast fungus Magnaporthe oryzae in Vietnamese rice cultivars

Cited by 10 publications

References 34 publications

The roles of rice microRNAs in rice-Magnaporthe oryzae interaction

The roles of rice microRNAs in rice-Magnaporthe oryzae interaction

Osa‐miR535 targets SQUAMOSApromoter binding protein‐like 4 to regulate blast disease resistance in rice

Fine-Tuning Roles of Osa-miR159a in Rice Immunity Against Magnaporthe oryzae and Development

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