Expression of p53 and Ki-67 in oral dysplasia and Squamous cell carcinoma: An immunohistochemical study

Patel, Sharmistha M.; Patel, Kinjal; Patel, Prashant; Gamit, Bhavna; Hathila, Rasik N.; Gupta, Swati

doi:10.5455/ijmsph.2014.030720144

Cited by 11 publications

(11 citation statements)

References 10 publications

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“…The association of pattern of staining of Ki-67 between these three groups was statisitically significant. These findings are in agreement to similar study by Humayun S et al 3 , Thomson PJ et al 23 , Santos-Garcia A et al 17 , Patel SM et al 15 , Kurokawa H et al 9 and Iamaroon et al 16 . Dwivedi N et al 10 have shown Ki-67 positive staining confined to parabasal layers, with lack of basal staining pattern of Ki-67, however, this finding was not seen in our study.…”

Section: Discussionsupporting

confidence: 92%

“…The p53 LI was more than 60.0% in 35.3% cases of OSCC. These findings are comparable with study by Humayun S et al 3 , Santos-Garcia A et al 17 , Swaminathan U et al 18 , Nasser W et al 19 , Iamaroon A et al 16 and Patel S et al 15 The increase in p53 expression through various grades of OSCC from Well differentiated SCC (WDSCC) to moderately differentiated SCC (MDSCC) to poorly differentiated SCC (PDSCC) has been highlighted by Verma R et al 20 Motta R et al have shown the importance of p53 overexpression in OSCC and regional lymph node metastasis and consequently worse prognosis 21 . Saranath D et al have depicted a 92.0% concordance between tissues showing p53 missense mutation and overexpression of p53 protein in oral cancer and leukoplakia, thus indicating p53 immunohistochemical analysis as a reliable surrogate marker for p53 missense mutation.…”

Section: Discussionsupporting

confidence: 90%

“…47.1% cases of SCC and 16.7% cases of keratosis with dysplasia showed p53 staining in all layers of epithelium, thus revealing extension of p53 expression from basal to suprabasal layers to all layers of epithelium with propagation of lesion towards carcinoma. These findings were concurrent with the study done by Humayun S et al 3 , Kurokawa H et al 9 , Patel SM et al 15 , Iamaroon A et al 16 Though a statistically significant difference between median p53 LI and keratosis without dysplasia, keratosis with dysplasia and OSCC was not observed in the study, the median p53 LI of SCC was more than those of keratosis without dysplasia and keratosis with dysplasia. The p53 LI was more than 60.0% in 35.3% cases of OSCC.…”

Section: Discussionsupporting

confidence: 88%

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Role of p53 and Ki-67 immunomarkers in oral premalignant lesions and squamous cell carcinoma: a hospital based study in BPKIHS

et al. 2018

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Background: As most of the oral squamous cell carcinoma develop from precursor premalignant lesions, it would be helpful if the malignant transformation is detected early in premalignant state. The objective of the research was to study the role of immunohistochemical expression of p53 and Ki-67 in oral premalignant lesion and squamous cell carcinoma.Materials and Methods: The expression of immunomarkers p53 and Ki67 were studied on formalin fixed paraffin embedded tissue sections from human oral squamous mucosal lesion for duration of 1 year. Results: Of total 36 cases, 80% cases of keratosis without dysplasia showed basal pattern of p53 staining while 47.1% cases of squamous cell carcinoma showed p53 staining in all layers of epithelium. The median p53 Labelling Index of squamous cell carcinoma was more than those of keratosis with and without dysplasia though the result was statistically non-significant. 50.0% cases of keratosis without dysplasia and 83.3% cases of keratosis with dysplasia displayed Ki-67 immunostaining confined to basal and suprabasal layer whereas 94.1% cases of squamous cell carcinoma showed Ki-67 positivity in all layers of epithelium. Median Ki-67 Labelling Index increased from keratosis without dysplasia to keratosis with dysplasia to squamous cell carcinoma, difference being statistically significant. A positive and insignificant correlation was observed between p53 and Ki-67 Labelling Index.Conclusion: Increased expressions of Ki-67 and p53 in oral squamous cell carcinoma compared to premalignant lesion suggest that they may be useful indicator of malignant transformation in dysplastic lesion.

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Section: Discussionsupporting

confidence: 92%

Section: Discussionsupporting

confidence: 90%

Section: Discussionsupporting

confidence: 88%

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Role of p53 and Ki-67 immunomarkers in oral premalignant lesions and squamous cell carcinoma: a hospital based study in BPKIHS

et al. 2018

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“…Squamous carcinomas represent about 3% of human cancers and more than 90% of malignant tumors at oral location are being diagnosed worldwide each year in more than 350,000 new cases. [1]…”

Section: Introductionmentioning

confidence: 99%

Analysis of Ki-67 expression in oral premalignant lesions and normal oral mucosa: An immunohistochemical study

et al. 2019

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Aim: This study aimed to evaluate the Ki-67 expression in oral premalignant lesions and normal oral mucosa. Materials and Methods: The cases were selected on the basis of the details obtained from the patients. A total of 45 specimens were divided into three groups: Group 1 (normal mucosa), Group 2 (clinically and histologically diagnosed as oral lichen planus), and Group 3 (clinically and histologically diagnosed as leukoplakia). Specimens were prepared and the slides for Ki-67 were observed under light microscope with a magnification of ×400. The tissue samples were thoroughly examined, and the pattern of expression was analyzed semiquantitatively by counting the number of positive cells. Results: The mean positive cell count of normal mucosa was 23.20 ± 2.89, of oral lichen planus was 42.82 ± 2.65, and of leukoplakia was 82.14 ± 3.10. There was a statistically significant difference of expression observed between the groups ( P < 0.001). On multiple comparisons using Tukey post hoc test, a statistical difference was found between all the three groups. Conclusion: Ki-67 is an easily applicable marker of cell proliferation whose expression correlates well with the disease progression.

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“…The percentage of leukoplakia that progresses to invasive OSCC is accepted to be directly related to the severity of dysplastic changes presumably due to the accumulation of genomic and molecular alterations. [5]…”

Section: Introductionmentioning

confidence: 99%

Expression of p53 protein and Ki-67 antigen in oral leukoplakia with different histopathological grades of epithelial dysplasia

Suwasini¹,

Chatterjee²,

Purkait³

et al. 2018

J Int Soc Prevent Communit Dent

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Aims and Objective:The aim of this study is to investigate the expression of p53 protein and Ki-67 antigen in normal oral mucosa and oral leukoplakia with different grades of dysplasia using immunohistochemistry and to clarify the correlation of the expression of these cell cycle regulatory proteins.Materials and Methods:A total of 20 archival tissue blocks obtained from our department which were diagnosed as mild (n = 5), moderate (n = 5), and severe dysplasia (n = 5) with normal mucosa (n = 5) as a control. Positivity of Ki-67 and p53 was analyzed using Statistical Package for the Social Sciences (IBM Corp. Released 2013. IBM SPSS Statistics for Windows, Version 22.0 Armonk, NY:IBM Corp) software.Results:All samples showed positive staining for p53 and Ki-67. Statistically significant difference (P < 0.05) was seen between the frequency of occurrence of p53 and Ki-67 pattern of expression among all the groups. The intensity of staining was mild to intense in basal layer as there was a progression toward the severity of the disease. Almost 81.1% correlation existed between p53 and Ki-67 with high correlation and marked relationship.Conclusion:Oral leukoplakia represents the most common oral potentially malignant disorder (OPMD). Molecular biological markers such as p53 and Ki-67 are considered to be of great value in the diagnosis and prognostic evaluation of OPMD. Our results emphasize the potential use of p53 protein and Ki-67 antigen as significant molecular markers for early detection of PMDs and its risk of developing oral squamous cell carcinoma.

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Expression of p53 and Ki-67 in oral dysplasia and Squamous cell carcinoma: An immunohistochemical study

Cited by 11 publications

References 10 publications

Role of p53 and Ki-67 immunomarkers in oral premalignant lesions and squamous cell carcinoma: a hospital based study in BPKIHS

Role of p53 and Ki-67 immunomarkers in oral premalignant lesions and squamous cell carcinoma: a hospital based study in BPKIHS

Analysis of Ki-67 expression in oral premalignant lesions and normal oral mucosa: An immunohistochemical study

Expression of p53 protein and Ki-67 antigen in oral leukoplakia with different histopathological grades of epithelial dysplasia

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