Expression of p53, Ki-67 and c-erb B2 in growth hormone-and/or prolactin-secreting pituitary adenomas

Botelho, Carlos Henrique Aguiar; Magalhães, Albino Verçosa de; Mello, Paulo Andrade de; Schmitt, Fernando; Casulari, Luíz Augusto

doi:10.1590/s0004-282x2006000100013

Cited by 33 publications

(21 citation statements)

References 20 publications

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“…The presence of p53 mutations in pituitary tumors is rarely described (28), but some authors found a relationship with proliferative states (29,30). In our patient we detected positivity in 10% of the tumoral cells.…”

Section: Discussionsupporting

confidence: 52%

Aggressive prolactinoma in a child related to germline mutation in the ARYL hydrocarbon receptor interacting protein (AIP) gene

Naves

Jaffrain-Rea

Vencio

et al. 2010

Arq Bras Endocrinol Metab

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SUMMARYThe objective of this study was to describe a familial screening for AIP mutations in the context of aggressive prolactinoma in childhood. A 12-year-old boy, presented headaches and bilateral hemianopsia. He had adequate height and weight for his age (50 th percentile), Tanner stage G1 P1. His bone age was 10 years. Prolactin was 10.560 ng/mL (3-25), FSH and LH were undetectable, IGF-1, TSH, Free T4, ACTH, and cortisol were within normal ranges. MRI showed a pituitary macroadenoma, 5.3 X 4.0 X 3.5 cm with compression of the optic chiasm, bilateral cavernous sinus invasion, encasement of carotids, and extension to clivus. Surgical debulking was performed. Resistance to cabergoline was characterized and he was submitted to two surgeries and radiotherapy. Immunohistochemical evaluation included prolactin, ACTH, GH, FSH, LH,AIP, c-erb B2, Ki-67, and p53. Genomic DNA was isolated from the index case and 48 relatives, PCR and sequencing were performed. A germline A195V mutation in AIP was identified in the index case and in five asymptomatic relatives. Germline mutations in the AIP gene may be involved in the predisposition to pituitary adenoma formation, as cause or co-factor in pathogenesis of aggressive tumors in young patients. Arq Bras Endocrinol Metab. 2010;54(8):761-7 SUMÁRIOO objetivo deste estudo foi descrever o rastreamento familiar para mutações AIP em paciente portador de prolactinoma agressivo e resistente na infância. Um menino de 12 anos foi avaliado com queixa de cefaleia e hemianopsia bitemporal. Apresentava peso e altura adequados para a idade (percentil 50), estádio puberal Tanner G1 P1 e idade óssea de 10 anos. Prolactina 10.560 ng/mL (3-25), FSH e LH indetectáveis, IGF-1, TSH, T4 livre, ACTH, e cortisol normais. A ressonância magnética de sela evidenciou macroadenoma hipofisário, 53 X 40 X 35 mm com compressão de quiasma ótico, invasão de seios cavernosos, envolvimento de carótidas internas e extensão para o clivus. Foi realizada descompressão cirúrgica por via transesfenoidal e caracterizada resistência a doses máximas de cabergolina, sendo o paciente operado por mais duas vezes e submetido à radioterapia. Realizou-se imuno-histoquímica para prolactina, ACTH, GH, FSH, LH, AIP, c-erb B2, Ki-67 e p53. O DNA genômico foi extraído do caso índice e de 48 familiares, e PCR e sequenciamento. Uma mutação A195V na AIP foi detectada no paciente e em cinco parentes assintomáticos. As mutações no gene da AIP podem estar envolvidas na predisposição à formação de adenomas, como causa ou cofator na patogênese de tumores agressivos em jovens. Arq Bras Endocrinol Metab. 2010;54(8):761-7

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Section: Discussionsupporting

confidence: 52%

Aggressive prolactinoma in a child related to germline mutation in the ARYL hydrocarbon receptor interacting protein (AIP) gene

Naves

Jaffrain-Rea

Vencio

et al. 2010

Arq Bras Endocrinol Metab

Self Cite

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“…Other studies have counted randomly selected areas [27] or used a method including 3 areas of the tumor with an even distribution of labeled cells [25]. Some studies have used only a small piece of the biopsy included in tissue microarrays [33,34].…”

Section: Discussionmentioning

confidence: 99%

Role of Ki-67 proliferation index and p53 expression in predicting progression of pituitary adenomas

2008

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“…Invasive adenomas can infiltrate the bone and rarely the brain (8). There is no parameter for treatment options and behavior pattern of this type of invasive pituitary adenoma.…”

Section: Discussionmentioning

confidence: 99%

Cavernous sinus invasion and effect of immunohistochemical features over remission in growth hormone secreting pituitary adenomas

Yılmaz

Vural²,

Koç³

et al. 2014

Turkish Neurosurgery

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AIM:We examined the cavernous sinus invasion and tumor biological markers that influence the remission rate. Cavernous sinus (CS) invasion was evaluated radiologically. Tumor biological markers consisting of the tumor cell growth parameter Ki-67 and the cancer cell vasculature marker of vascular endothelial growth factor (VEGF) were evaluated. MATErIAL and METHods:We examined 28 immunohistochemically proven GH secreting pituitary adenoma patients who had been operated via endoscopic transsphenoidal surgery at Department of Neurosurgery, Kocaeli University Hospital between 2003 and 2008. Pathology preparations were stained with Ki-67 and VEGF. We evaluated remission at the postoperative 6 th week. The basal GH level, nadir GH level and IGF-1 levels were evaluated to determine remission. rEsuLTs:Remission was achieved in 6 of 18 patients (33%) who had cavernous sinus invasion. Remission was achieved in 7 of 10 patients (70%) who did not have cavernous sinus invasion. There was no correlation between the Ki-67 proliferation index and cavernous sinus invasion (p=0.593). There was a positive correlation between VEGF expression and cavernous sinus invasion (p=0.03). CoNCLusIoN:The remission rate found less in the cavernous sinus invasion group. No association was found between Ki 67 proliferation index and cavernous sinus invasion. We found that a positive correlation between VEGF expression and cavernous sinus invasion. VEGF expression can therefore indirectly affect remission via cavernous sinus invasion.KEywords: GH adenoma, VEGF, Ki-67, Cavernous sinus invasion, Remission ÖZ AMAÇ: Çalışmada remisyona etkisi olduğu öngörülen faktörlerden; kavernöz sinüs invazyonu ve tümör biyolojisi ile ilgili özellikler gözden geçirildi. Bu amaçla kavernöz sinüs invazyonu radyolojik olarak değerlendirildi. Ayrıca tümör biyolojisini gösteren; tümör büyüme potansiyelinde etkili Ki-67 ve tümör damarlanmasını gösteren VEGF immünohistokimyasal olarak incelendi. BuLGuLAr: Kavernöz sinüs invazyonu olan hastalarda kısa dönem remisyon oranı 6/18 (%33) idi. Kavernöz sinüs invazyonu olmayan 10 hastanın 7'sinde remisyon sağlandı (%70). Ki-67 proliferasyon indeksi ile kavernöz sinüs invazyonu arasında anlamlı bir korelasyon olmadığı (p=0,593), VEGF ekspresyonunun kavernöz sinüs invazyonu açısından önemli bir gösterge olduğu (p=0,03) saptandı. soNuÇ: Kavernöz sinüs invazyonu gösteren GH adenomu olgularında kısa dönem remisyon oranlarının daha düşük görüldüğü, Ki-67'nin kavernöz sinüs invazyonu ve remisyonu etkilemediği, VEGF ekspresyonunun kavernöz sinüs invazyonunu artırarak indirekt yolla remisyon üzerinde etkili olabileceği sonucuna varıldı.

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Expression of p53, Ki-67 and c-erb B2 in growth hormone-and/or prolactin-secreting pituitary adenomas

Cited by 33 publications

References 20 publications

Aggressive prolactinoma in a child related to germline mutation in the ARYL hydrocarbon receptor interacting protein (AIP) gene

Aggressive prolactinoma in a child related to germline mutation in the ARYL hydrocarbon receptor interacting protein (AIP) gene

Role of Ki-67 proliferation index and p53 expression in predicting progression of pituitary adenomas

Cavernous sinus invasion and effect of immunohistochemical features over remission in growth hormone secreting pituitary adenomas

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