Expression of phenol oxidase and heat-shock genes during the development of Agaricus bisporus fruiting bodies, healthy and infected by Lecanicillium fungicola

Largeteau, M.; Latapy, Camille; Minvielle, Nathalie; Régnault-Roger, Catherine; Savoie, Jean‐Michel

doi:10.1007/s00253-009-2186-2

Cited by 20 publications

(16 citation statements)

References 34 publications

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“…Glycoside hydrolases catalyze the hydrolysis of the glycosidic linkage to release smaller sugars and play significant role in biomass degradation by some fungi [38]. Heat shock proteins have been reported to protect the cells against stress conditions and be potentially involved in host-pathogen interaction [77]. Enolase, glucose-6-phosphate isomerase and tansketolase, associated with sugar metabolism, are important in both developmental processes.…”

Section: Resultsmentioning

confidence: 99%

Genome-Wide Transcriptome and Proteome Analysis on Different Developmental Stages of Cordyceps militaris

Yin

Chen

et al. 2012

PLoS ONE

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Background Cordyceps militaris, an ascomycete caterpillar fungus, has been used as a traditional Chinese medicine for many years owing to its anticancer and immunomodulatory activities. Currently, artificial culturing of this beneficial fungus has been widely used and can meet the market, but systematic molecular studies on the developmental stages of cultured C. militaris at transcriptional and translational levels have not been determined.Methodology/Principal FindingsWe utilized high-throughput Illumina sequencing to obtain the transcriptomes of C. militaris mycelium and fruiting body. All clean reads were mapped to C. militaris genome and most of the reads showed perfect coverage. Alternative splicing and novel transcripts were predicted to enrich the database. Gene expression analysis revealed that 2,113 genes were up-regulated in mycelium and 599 in fruiting body. Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) analysis were performed to analyze the genes with expression differences. Moreover, the putative cordycepin metabolism difference between different developmental stages was studied. In addition, the proteome data of mycelium and fruiting body were obtained by one-dimensional gel electrophoresis (1-DGE) coupled with nano-electrospray ionization liquid chromatography tandem mass spectrometry (nESI-LC-MS/MS). 359 and 214 proteins were detected from mycelium and fruiting body respectively. GO, KEGG and Cluster of Orthologous Groups (COG) analysis were further conducted to better understand their difference. We analyzed the amounts of some noteworthy proteins in these two samples including lectin, superoxide dismutase, glycoside hydrolase and proteins involved in cordycepin metabolism, providing important information for further protein studies.Conclusions/SignificanceThe results reveal the difference in gene expression between the mycelium and fruiting body of artificially cultivated C. militaris by transcriptome and proteome analysis. Our study provides an effective resource for the further developmental and medicinal research of this promising fungus.

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Section: Resultsmentioning

confidence: 99%

Genome-Wide Transcriptome and Proteome Analysis on Different Developmental Stages of Cordyceps militaris

Yin

Chen

et al. 2012

PLoS ONE

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“…Moreover, there appears to be a natural laccase substrate in the gills [274]. In A. bisporus , it is in the primordia (‘ pin stage ’) where expression of gene lcc1 increases in accordance to detected enzymatic activity [275,276] while lcc1 and lcc3 transcription was reported to decrease in the vegetative mycelium with onset of fruiting [95]. Further expressed in different stages of sporophore morphogenesis is laccase gene lcc3 .…”

Section: Functions Of Laccases Sensu Stricto In Basidiomycetesmentioning

confidence: 99%

“…Further expressed in different stages of sporophore morphogenesis is laccase gene lcc3 . This gene is also expressed in ‘ bubbles ’, masses of undifferentiated tissues produced instead of mushrooms upon fungal pathogen infection [276]. …”

Section: Functions Of Laccases Sensu Stricto In Basidiomycetesmentioning

confidence: 99%

Multiple Multi-Copper Oxidase Gene Families in Basidiomycetes – What for?

Kües¹,

Rühl

2011

124

125

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Genome analyses revealed in various basidiomycetes the existence of multiple genes for blue multi-copper oxidases (MCOs). Whole genomes are now available from saprotrophs, white rot and brown rot species, plant and animal pathogens and ectomycorrhizal species. Total numbers (from 1 to 17) and types of mco genes differ between analyzed species with no easy to recognize connection of gene distribution to fungal life styles. Types of mco genes might be present in one and absent in another fungus. Distinct types of genes have been multiplied at speciation in different organisms. Phylogenetic analysis defined different subfamilies of laccases sensu stricto (specific to Agaricomycetes), classical Fe2+-oxidizing Fet3-like ferroxidases, potential ferroxidases/laccases exhibiting either one or both of these enzymatic functions, enzymes clustering with pigment MCOs and putative ascorbate oxidases. Biochemically best described are laccases sensu stricto due to their proposed roles in degradation of wood, straw and plant litter and due to the large interest in these enzymes in biotechnology. However, biological functions of laccases and other MCOs are generally little addressed. Functions in substrate degradation, symbiontic and pathogenic intercations, development, pigmentation and copper homeostasis have been put forward. Evidences for biological functions are in most instances rather circumstantial by correlations of expression. Multiple factors impede research on biological functions such as difficulties of defining suitable biological systems for molecular research, the broad and overlapping substrate spectrum multi-copper oxidases usually possess, the low existent knowledge on their natural substrates, difficulties imposed by low expression or expression of multiple enzymes, and difficulties in expressing enzymes heterologously.

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“…EF1-a gene (Genbank 997204) encoding the basidiomycete elongation factor 1-alpha was used as the reference gene (Largeteau et al 2010). Specific primers for PPO1 gene and EF1-a gene were: PPO1-forward (5 0 -GGGTGTGAACGCAAAGGATA A-3 0 ) and PPO1-reverse (5 0 -GTATGGCTGCTGAA ATGAGGC-3 0 ); EF1-a-forward (5 0 -GGTCGTGTTG AGACTGGTA-3 0 ) and EF1-a-reverse (5 0 -GGGTCGT TCTTGGAATCAGA-3 0 ).…”

Section: Estimation Of Rna Purity Yield and Integritymentioning

confidence: 99%

Isolation of high quality and yield of RNA from Agaricus bisporus with a simple, inexpensive and reliable method

et al. 2012

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A method for isolating high purity and quantity RNA from Agaricus bisporus which is rich in proteins, carbohydrate, fiber and secondary metabolites, is described. RNA was extracted from mycelium, primordia, sporophores at two development stages and two post-harvest storage stages as well as from pileipellis, inner cap, gill and stipe of the mature sporophore. The A(260)/A(230) and A(260)/A(280) ratios of isolated RNA from fruiting bodies were both ~2 and the yield was about 200 μg/g fresh wt (FW). The yield of RNA from mycelium was approx. 100 μg/g FW. High quality RNA was also extracted from fruiting body tissues of Lentinus edodes, Pleurotus ostreatus, Flammulina velutipes and Pleurotus eryngii with yields from 130 to 225 μg/g FW. RNA extracted from all samples was intact, as demonstrated by gel electrophoresis and was suitable for downstream molecular applications, including RT-PCR and qPCR.

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Expression of phenol oxidase and heat-shock genes during the development of Agaricus bisporus fruiting bodies, healthy and infected by Lecanicillium fungicola

Cited by 20 publications

References 34 publications

Genome-Wide Transcriptome and Proteome Analysis on Different Developmental Stages of Cordyceps militaris

Genome-Wide Transcriptome and Proteome Analysis on Different Developmental Stages of Cordyceps militaris

Multiple Multi-Copper Oxidase Gene Families in Basidiomycetes – What for?

Isolation of high quality and yield of RNA from Agaricus bisporus with a simple, inexpensive and reliable method

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