Expression of placenta-specific 8 in human oocytes, embryos, and models of in vitro implantation

Li, Min; Liu, Dong; Wang, Li; Wang, Weizhou; Wang, Aiming; Yao, Yuanqing

doi:10.1016/j.fertnstert.2016.05.018

Cited by 22 publications

(19 citation statements)

References 27 publications

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“…The downregulated BIRC5 induced by BoNTA indicated the decrease of apoptosis in HDFs. PLAC8 has been proved to regulate cell cycle and participate in the regulation of apoptosis and cell division [ 29 – 31 ].…”

Section: Discussionmentioning

confidence: 99%

The Effect of Botulinum Toxin Type A on Expression Profiling of Long Noncoding RNAs in Human Dermal Fibroblasts

Miao

Liu

Zhu

et al. 2017

BioMed Research International

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Objective. This study was aimed at analyzing the expressions of long noncoding RNAs (lncRNAs) in Botulinum Toxin Type A (BoNTA) treated human dermal fibroblasts (HDFs) in vitro. Methods. We used RNA sequencing to characterize the lncRNAs and mRNAs transcriptome in the control and BoNTA treated group, in conjunction with application of GO (gene ontology) analysis and KEGG (kyoto encyclopedia of genes and genomes) analysis to delineate the alterations in gene expression. We also obtained quantitative real time polymerase chain reaction (qRT-PCR) to confirm some differentially expressed genes. Results. Numerous differentially expressed genes were observed by microarrays between the two groups. qRT-PCR confirmed the changes of six lncRNAs (RP11-517C16.2-001, FR271872, LOC283352, RP11-401E9.3, FGFR3P, and XXbac-BPG16N22.5) and nine mRNAs (NOS2, C13orf15, FOS, FCN2, SPINT1, PLAC8, BIRC5, NOS2, and COL19A1). Farther studies indicated that the downregulating effect of BoNTA on the expression of FGFR3P was time-related and the dosage of BoNTA at a range from 2.5 U/106 cells to 7.5 U/106 cells increased the expression of FGFR3P and COL19A1 in HDFs as well. Conclusion. The expression profiling of lncRNAs was visibly changed in BoNTA treated HDFs. Further studies should focus on several lncRNAs to investigate their functions in BoNTA treated HDFs and the underlying mechanisms.

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Section: Discussionmentioning

confidence: 99%

The Effect of Botulinum Toxin Type A on Expression Profiling of Long Noncoding RNAs in Human Dermal Fibroblasts

Miao

Liu

Zhu

et al. 2017

BioMed Research International

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“…In plants, PCR proteins have been found to associate to the plasma membrane, where they regulate metal transport 17,18 . More complex is the localization of PLAC8 proteins in mammalian cells, where Onzin in particular has been observed in the nucleus 22 , in the cytoplasm 7,23 , in the lysosomal compartment 24 or associated to the plasma membrane 4,25 . Interestingly, Onzin could be dynamically redistributed between the nucleus, the cytoplasm and the plasma membrane depending on the growth conditions and the relative abundance of interacting proteins 2 .…”

Section: Resultsmentioning

confidence: 99%

Yeast expression of mammalian Onzin and fungal FCR1 suggests ancestral functions of PLAC8 proteins in mitochondrial metabolism and DNA repair

Daghino

Vietro

Petiti

et al. 2019

Sci Rep

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The cysteine-rich PLAC8 domain of unknown function occurs in proteins found in most Eukaryotes. PLAC8-proteins play important yet diverse roles in different organisms, such as control of cell proliferation in animals and plants or heavy metal resistance in plants and fungi. Mammalian Onzin can be either pro-proliferative or pro-apoptotic, depending on the cell type, whereas fungal FCR1 confers cadmium tolerance. Despite their different role in different organisms, we hypothesized common ancestral functions linked to the PLAC8 domain. To address this hypothesis, and to investigate the molecular function of the PLAC8 domain, murine Onzin and fungal FCR1 were expressed in the PLAC8-free yeast Saccharomyces cerevisiae . The two PLAC8-proteins localized in the nucleus and induced almost identical phenotypes and transcriptional changes when exposed to cadmium stress. Like FCR1, Onzin also reduced DNA damage and increased cadmium tolerance by a DUN1-dependent pathway. Both proteins activated transcription of ancient mitochondrial pathways such as leucine and Fe-S cluster biosynthesis, known to regulate cell proliferation and DNA repair in yeast. These results strongly suggest a common ancestral function of PLAC8 proteins and open new perspectives to understand the role of the PLAC8 domain in the cellular biology of Eukaryotes.

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“…The estrogens are secreted at two periods during implantation: (1) an increase in secretion between days 11 and 12 of gestation, and (2) a sustained increase in secretion from day 15 to 25 of gestation to initial attachment of conceptus to the endometrial luminal epithelium [66][67][68]. Previous studies showed that E2 can induce the expression of SULT2A1 and SPP1 but has an effect to downregulate the expression of MEP1B, and the effect of the estrogens is restricted to the region in close proximity to conceptus [10,35,[69][70][71]. These results may explain the spatial-restricted expression manner of SULT2A1 and SPP1 in the uterus.…”

Section: Discussionmentioning

confidence: 99%

“…Therefore, six types of samples (3 compartments per gestational day) were used for RNA-sequencing and a total of 15,062 genes were detected. Genes that have been reported to be expressed in pig endometrial luminal epithelium (such as SPP1 (Secreted phosphoprotein 1) [27,28], MUC1 (Mucin 1) [29] and STC1 (Stanniocalcin 1) [30]) were included, and pig conceptus-expressed genes including IFNG (Interferon gamma) [31], PLET1 (Placenta expressed transcript 1) [32], PLAC1 (Placenta-specific 1) [33] and PLAC8 (Placenta-specific 8) [34,35] were also detected. Descriptive statistics of the RNA-seq data are given in Table S1.…”

Section: Transcriptomic Profiles Of Three Spatially Distinct Compartmmentioning

confidence: 99%

Integrating LCM-Based Spatio-Temporal Transcriptomics Uncovers Conceptus and Endometrial Luminal Epithelium Communication that Coordinates the Conceptus Attachment in Pigs

Wang

Zhao

Deng

et al. 2021

IJMS

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Attachment of conceptus to the endometrial luminal epithelium (LE) is a critical event for early placentation in Eutheria. Since the attachment occurs at a particular site within the uterus, a coordinated communication between three spatially distinct compartments (conceptus and endometrial LE from two anatomical regions of the uterus to which conceptus attaches and does not attach) is essential but remains to be fully characterized. Using the laser capture microdissection (LCM) technique, we firstly developed an approach that can allow us to pair the pig conceptus sample with its nearby endometrial epithelium sample without losing the native spatial information. Then, a comprehensive spatio-temporal transcriptomic profile without losing the original conceptus-endometrium coordinates was constructed. The analysis shows that an apparent difference in transcriptional responses to the conceptus exists between the endometrial LE from the two anatomically distinct regions in the uterus. In addition, we identified the communication pathways that link the conceptus and endometrial LE and found that these pathways have important roles in conceptus attachment. Furthermore, a number of genes whose expression is spatially restricted in the two different anatomical regions within the uterus were characterized for the first time and two of them (SULT2A1 and MEP1B) may cooperatively contribute to establish conceptus attachment in pigs. The results from our study have implications in understanding of conceptus/embryo attachment in pigs and other large polytocous species.

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Expression of placenta-specific 8 in human oocytes, embryos, and models of in vitro implantation

Cited by 22 publications

References 27 publications

The Effect of Botulinum Toxin Type A on Expression Profiling of Long Noncoding RNAs in Human Dermal Fibroblasts

The Effect of Botulinum Toxin Type A on Expression Profiling of Long Noncoding RNAs in Human Dermal Fibroblasts

Yeast expression of mammalian Onzin and fungal FCR1 suggests ancestral functions of PLAC8 proteins in mitochondrial metabolism and DNA repair

Integrating LCM-Based Spatio-Temporal Transcriptomics Uncovers Conceptus and Endometrial Luminal Epithelium Communication that Coordinates the Conceptus Attachment in Pigs

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