Expression of plasma membrane H<sup>+</sup>‐ATPase in cluster roots of white lupin under phosphorus deficiency

To understand the regulation of roots plasma membrane H+-ATPase in Masson pine responding to acid deposition, the changes in biomass, plant morphology, intracellular H+, enzyme activity and H+-ATPase genes expression in Masson pine seedlings exposed to simulated acid rain (SAR, pH 5.6 and 4.6) with and without vanadate were studied. SAR exposure for 60 days increased the intracellular H+ in pine roots whether added with 0.1 mM Na3VO4 or not. The growth of seedlings treated with SAR maintained well, even the primary lateral root length, root dry weight, and number of root tips in seedlings exposed to SAR at pH 4.6 were higher than that of the control (pH 6.6). However, the addition of vanadate resulted in the severe growth inhibition and obvious decline in morphological parameters. Similarly, ATP hydrolytic activity and H+ transport activity of roots plasma membrane H+-ATPase both were stimulated by SAR whereas inhibited by vanadate, and the highest activity stimulation was observed in pine roots subjected to SAR at pH 4.6. In addition, SAR also induced the expression of the investigated H+-ATPase subunits (atpB, atpE, atpF, atpH, and atpI). Therefore, the roots plasma membrane H+-ATPase is instrumental in the growth of Masson pine seedlings adapting to acid rain by a manner of pumping more protons across the membrane through enhancing its activity, and which involves the up-regulated gene expression of roots H+-ATPase subunits at transcriptional level.

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Multi-locus genome-wide association study for phosphorus use efficiency in a tropical maize germplasm

Zeffa,

Júnior,

de Assis

et al. 2024

Front. Plant Sci.

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Phosphorus (P) is an essential macronutrient for maize (Zea mays L.) growth and development. Therefore, generating cultivars with upgraded P use efficiency (PUE) represents one of the main strategies to reduce the global agriculture dependence on phosphate fertilizers. In this work, genome-wide association studies (GWAS) were performed to detect quantitative trait nucleotide (QTN) and potential PUE-related candidate genes and associated traits in greenhouse and field trials under contrasting P conditions. The PUE and other agronomy traits of 132 maize inbred lines were assessed in low and normal P supply through the greenhouse and field experiments and Multi-locus GWAS was used to map the associated QTNs. Wide genetic variability was observed among the maize inbred lines under low and normal P supply. In addition, we confirm the complex and quantitative nature of PUE. A total of 306 QTNs were associated with the 24 traits evaluated using different multi-locus GWAS methods. A total of 186 potential candidate genes were identified, mainly involved with transcription regulator, transporter, and transference activity. Further studies are still needed to elucidate the functions and relevance of these genes regarding PUE. Nevertheless, pyramiding the favorable alleles pinpointed in the present study can be considered an efficient strategy for molecular improvement to increase maize PUE.

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Expression of plasma membrane H⁺‐ATPase in cluster roots of white lupin under phosphorus deficiency

Cited by 3 publications

References 12 publications

Transcriptional and physiological analyses uncover the mineralization and uptake mechanisms of phytic acid in symbiotically grown Vicia faba plants

Transcriptional and physiological analyses uncover the mineralization and uptake mechanisms of phytic acid in symbiotically grown Vicia faba plants

Modification of plasma membrane H+-ATPase in Masson pine (Pinus massoniana Lamb.) seedling roots adapting to acid deposition

Multi-locus genome-wide association study for phosphorus use efficiency in a tropical maize germplasm

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Expression of plasma membrane H+‐ATPase in cluster roots of white lupin under phosphorus deficiency

Cited by 3 publications

References 12 publications

Transcriptional and physiological analyses uncover the mineralization and uptake mechanisms of phytic acid in symbiotically grown Vicia faba plants

Transcriptional and physiological analyses uncover the mineralization and uptake mechanisms of phytic acid in symbiotically grown Vicia faba plants

Modification of plasma membrane H+-ATPase in Masson pine (Pinus massoniana Lamb.) seedling roots adapting to acid deposition

Multi-locus genome-wide association study for phosphorus use efficiency in a tropical maize germplasm

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Expression of plasma membrane H⁺‐ATPase in cluster roots of white lupin under phosphorus deficiency