Expression of Potential Regulatory Genes in Abdominal Adipose Tissue of Broiler Chickens during Early Development

Abstract

The identities of genes that underlie population variation in adipose tissue development in farm animals are poorly understood. Previous studies in our laboratory have suggested that increased fat tissue involves the expression modulation of an array of genes in broiler chickens. Of special interest are eight genes, FGFR3, EPHB2, IGFBP2, GREM1, TNC, COL3A1, ACBD7, and SCD. To understand their expression regulation and response to dietary manipulation, we investigated their mRNA levels after dietary manipulation during early development. Chickens were fed either a recommended standard or a high caloric diet from hatch to eight weeks of age (WOA). The high caloric diet markedly affected bodyweight of the broiler birds. mRNA levels of the eight genes in the abdominal adipose tissue were assayed at 2, 4, 6, and 8 WOA using RT-qPCR. Results indicate that (1) FGFR3 mRNA level was affected significantly by diet, age, and diet:age interaction; (2) COL3A mRNA level was repressed by high caloric diet; (3) mRNA levels of EPHB2, ACBD7, and SCD were affected by age; (4) mRNA level of TNC was modulated by age:diet interaction; (5) changes in GREM1 and IGFBP2 mRNA levels were not statistically different.

Genomic Insights Into the Multiple Factors Controlling Abdominal Fat Deposition in a Chicken Model

“…The candidate gene approach is another popular approach for QTL identification. Functions of genes are excellent candidates for linkage relationships with quantitative traits of important traits (Zhu and Zhao, 2007 ; Bohannon-Stewart et al, 2014 ). In general, the candidate gene approach has been widely used in the detection of genes responsible for disease and quantitative traits as well as evolutionary genomics, and has revealed important findings.…”

“…A previous study on broiler adipose tissue development investigated the effect of high-caloric diet vs. standard diet on mRNA expression of 8 candidate genes [ fibroblast growth factor receptor 3 ( FGFR3), EPH receptor B2 (EPHB2), insulin like growth factor binding protein 2 ( IGFBP2 ) , gremlin 1, DAN family BMP antagonist ( GREM1 ) , tenascin C ( TNC ) , collagen type III alpha 1 chain ( COL3A1 ) , acyl-CoA binding domain containing 7 ( ACBD7 ), and stearoyl-CoA desaturase ( SCD )] in abdominal fat at 2, 4, 6, and 8 wk of age. FGFR3 expression was affected significantly by diet, age, and diet:age interaction; COL3A expression was down-regulated by high-caloric diet; expression of EPHB2, ACBD7 , and SCD were affected by age; expression of TNC was modulated by age:diet interaction; changes in GREM1 and IGFBP2 expressions were statistically similar (Bohannon-Stewart et al, 2014 ).…”

The effects of conjugated linoleic acid isomers on the morphological changes in adipose tissue and adipogenic genes expressions on primary adipose tissue

“…These target genes were selected as they play central roles in fat deposition where LPL is involved in the regulation of lipid metabolism (Hermier, 1997). ACBD families act as an intracellular acyl-CoA pool formers and transporters that play critical roles in lipid metabolism and energy homeostasis (Stewart et al, 2014).…”

Involvement of IGFBP5 in the Development of Goose Fatty Liver via p38 Mitogen-Activated Protein Kinase (MAPK)

“…The liver plays an important role in insulin-mediated regulation of metabolism, especially through glucose and lipid homeostasis [1,2]. It is also a major site for the synthesis of endocrine factors, such as insulin-like growth factors 1 and 2 (IGF1 and IGF2) and their binding proteins (IGF-binding proteins, IGFBPs) [3].…”